植物RHO相关蛋白GTPases(RHO-related GTPases of plants,ROPs)是广泛存在于植物中的一类信号转导G蛋白(又称GTP结合蛋白),其通过结合GDP或GTP在非活性和活性状态间进行切换,进而在细胞极性控制、形态发育、激素水平调控、逆境反应等诸...植物RHO相关蛋白GTPases(RHO-related GTPases of plants,ROPs)是广泛存在于植物中的一类信号转导G蛋白(又称GTP结合蛋白),其通过结合GDP或GTP在非活性和活性状态间进行切换,进而在细胞极性控制、形态发育、激素水平调控、逆境反应等诸多植物生命活动的信号转导过程中扮演重要的分子开关角色。本文对ROP蛋白的结构域及基于蛋白质结构分类进行了介绍,并对拟南芥、玉米、水稻和大麦中的ROP家族蛋白质进行了系统进化分析。分析结果表明,这些植物中的ROP蛋白根据蛋白质结构域组成可分为Ⅰ类(typeⅠ)和Ⅱ类(typeⅡ)两种类型,而根据蛋白质序列的保守性可将其在植物中的ROP蛋白划分为4个进化枝。本综述不但对ROP蛋白作为分子开关在细胞内调控各种信号通路的机制进行了叙述,还对ROP在花粉管、根毛及植物表皮铺盖细胞极性发育,以及其他抗逆反应中的具体作用和机制及研究进展进行了阐述。本文还对ROP蛋白在ABA、IAA、BR等植物激素信号传导过程中的调控作用及研究进展进行了阐述。本文对植物ROP蛋白研究过程中尚未解决的问题,例如不同的ROP蛋白在同一个信号通路中的作用为何如此不同,以及ROP是如何协调不同的信号通路以共同调控一个植物发育或者生理过程等问题进行了总结,并在此基础上对未来的研究方向进行了展望。展开更多
The CDEX collaboration has been established for direct detection of light dark matter particles, using ultra-low energy threshold point-contact p-type germanium detectors, in China JinPing underground Laboratory （CJP...The CDEX collaboration has been established for direct detection of light dark matter particles, using ultra-low energy threshold point-contact p-type germanium detectors, in China JinPing underground Laboratory （CJPL）. The first 1 kg point-contact germanium detector with a sub-keV energy threshold has been tested in a passive shielding system located in CJPL. The outputs from both the point-contact P＋ electrode and the outside N＋ electrode make it possible to scan the lower energy range of less than 1 keV and at the same time to detect the higher energy range up to 3 MeV. The outputs from both P＋ and N＋ electrode may also provide a more powerful method for signal discrimination for dark matter experiment. Some key parameters, including energy resolution, dead time, decay times of internal X-rays, and system stability, have been tested and measured. The results show that the 1 kg point-contact germanium detector, together with its shielding system and electronics, can run smoothly with good performances. This detector system will be deployed for dark matter search experiments.展开更多
The China Dark Matter Experiment （CDEX） is located at the China Jinping Underground Laboratory （CJPL） and aims to directly detect the weakly interacting massive particles （WIMP） flux with high sensitivity in the...The China Dark Matter Experiment （CDEX） is located at the China Jinping Underground Laboratory （CJPL） and aims to directly detect the weakly interacting massive particles （WIMP） flux with high sensitivity in the low mass region. Here we present a study of tile predicted photon and electron backgrounds including the background contribution of the structure materials of the germanium detector, the passive shielding materials, and the intrinsic radioactivity of the liquid argon that serves as an anti-Compton active shielding detector. A detailed geometry is modeled and the background contribution has been simulated based on the measured radioactivities of all possible components within tile GEANT4 program. Then the photon and electron background level in the energy region of interest （〈10-2events-kg1·day 1·keV-1 （cpkkd）） is predicted based on Monte Carlo simulations. The simulated result is consistent with the design goal of the CDEX-10 experiment, 0.1cpkkd, which shows that the active and passive shield design of CDEX-10 is effective and feasible.展开更多
文摘植物RHO相关蛋白GTPases(RHO-related GTPases of plants,ROPs)是广泛存在于植物中的一类信号转导G蛋白(又称GTP结合蛋白),其通过结合GDP或GTP在非活性和活性状态间进行切换,进而在细胞极性控制、形态发育、激素水平调控、逆境反应等诸多植物生命活动的信号转导过程中扮演重要的分子开关角色。本文对ROP蛋白的结构域及基于蛋白质结构分类进行了介绍,并对拟南芥、玉米、水稻和大麦中的ROP家族蛋白质进行了系统进化分析。分析结果表明,这些植物中的ROP蛋白根据蛋白质结构域组成可分为Ⅰ类(typeⅠ)和Ⅱ类(typeⅡ)两种类型,而根据蛋白质序列的保守性可将其在植物中的ROP蛋白划分为4个进化枝。本综述不但对ROP蛋白作为分子开关在细胞内调控各种信号通路的机制进行了叙述,还对ROP在花粉管、根毛及植物表皮铺盖细胞极性发育,以及其他抗逆反应中的具体作用和机制及研究进展进行了阐述。本文还对ROP蛋白在ABA、IAA、BR等植物激素信号传导过程中的调控作用及研究进展进行了阐述。本文对植物ROP蛋白研究过程中尚未解决的问题,例如不同的ROP蛋白在同一个信号通路中的作用为何如此不同,以及ROP是如何协调不同的信号通路以共同调控一个植物发育或者生理过程等问题进行了总结,并在此基础上对未来的研究方向进行了展望。
基金Supported by National Natural Science Foundation of China(10935005,10945002,11275107,11175099)National Basic Research Program of China(973 Program)(2010CB833006)
文摘The CDEX collaboration has been established for direct detection of light dark matter particles, using ultra-low energy threshold point-contact p-type germanium detectors, in China JinPing underground Laboratory （CJPL）. The first 1 kg point-contact germanium detector with a sub-keV energy threshold has been tested in a passive shielding system located in CJPL. The outputs from both the point-contact P＋ electrode and the outside N＋ electrode make it possible to scan the lower energy range of less than 1 keV and at the same time to detect the higher energy range up to 3 MeV. The outputs from both P＋ and N＋ electrode may also provide a more powerful method for signal discrimination for dark matter experiment. Some key parameters, including energy resolution, dead time, decay times of internal X-rays, and system stability, have been tested and measured. The results show that the 1 kg point-contact germanium detector, together with its shielding system and electronics, can run smoothly with good performances. This detector system will be deployed for dark matter search experiments.
基金Supported by National Natural Science Foundation of China(11175099,10935005,10945002,11275107,11105076)State Key Development Program of Basic Research of China(2010CB833006)
文摘The China Dark Matter Experiment （CDEX） is located at the China Jinping Underground Laboratory （CJPL） and aims to directly detect the weakly interacting massive particles （WIMP） flux with high sensitivity in the low mass region. Here we present a study of tile predicted photon and electron backgrounds including the background contribution of the structure materials of the germanium detector, the passive shielding materials, and the intrinsic radioactivity of the liquid argon that serves as an anti-Compton active shielding detector. A detailed geometry is modeled and the background contribution has been simulated based on the measured radioactivities of all possible components within tile GEANT4 program. Then the photon and electron background level in the energy region of interest （〈10-2events-kg1·day 1·keV-1 （cpkkd）） is predicted based on Monte Carlo simulations. The simulated result is consistent with the design goal of the CDEX-10 experiment, 0.1cpkkd, which shows that the active and passive shield design of CDEX-10 is effective and feasible.
