目的探讨大鼠骨髓间充质干细胞(BMSCs)异体移植对小鼠实验性自身免疫性脑脊髓炎(EAE)的治疗作用。方法全骨髓贴壁培养法获得大鼠BMSCs,流式细胞术检测细胞免疫表型,并诱导成骨方向分化。取雌性C57BL/6小鼠,随机分为3组:正常对照组、PBS...目的探讨大鼠骨髓间充质干细胞(BMSCs)异体移植对小鼠实验性自身免疫性脑脊髓炎(EAE)的治疗作用。方法全骨髓贴壁培养法获得大鼠BMSCs,流式细胞术检测细胞免疫表型,并诱导成骨方向分化。取雌性C57BL/6小鼠,随机分为3组:正常对照组、PBS组和大鼠BMSCs组,用髓鞘少突胶质细胞糖蛋白(MOG)35~55联合完全弗氏佐剂诱导建立EAE模型。小鼠免疫后38d和48d腹腔注射PBS或大鼠BMSCs进行治疗,神经功能评分观察各组小鼠神经功能变化。二次治疗12d后取各组小鼠脊髓、脾脏和外周血。HE染色及Luxol fast blue染色观察脊髓炎性细胞浸润及髓鞘脱失情况;脾脏制成单细胞悬液,细胞CFSE标记后10mg/L刀豆球蛋白(Con A)和MOG_(35~55)刺激培养3d,观察脾细胞增殖情况。ELISA检测大鼠BMSCs移植后外周血细胞因子干扰素γ(IFN-γ)、白细胞介素17(IL-17)含量。结果流式细胞术显示,大鼠BMSCs第3代(P3)细胞表达抗原CD29、CD90、CD106,不表达CD45。体外诱导其能向成骨分化。小鼠发病后,大鼠BMSCs组小鼠神经功能缺损症状较PBS组减轻,评分降低。HE染色和Luxol fast blue染色结果显示,大鼠BMSCs组脊髓炎细胞浸润和脱髓鞘比同时间点PBS组减轻(P<0.05)。Con A和MOG_(35~55)刺激培养后,PBS组和大鼠BMSCs组脾细胞增殖增加,而大鼠BMSCs组又较PBS组降低。与PBS组相比,大鼠BMSCs组血浆细胞因子IFN-γ、IL-17含量降低(P<0.05)。结论全骨髓贴壁培养法能有效分离纯化BMSCs,大鼠BMSCs异体移植对小鼠EAE模型有治疗作用。展开更多
IO Cnc was classified to be a new G-type(G0)W UMa-type eclipsing binary system.Our first multicolor photometric solutions show that IO Cnc is a new W-subtype shallow contact binary with a fill-out factor of f=16.1%and...IO Cnc was classified to be a new G-type(G0)W UMa-type eclipsing binary system.Our first multicolor photometric solutions show that IO Cnc is a new W-subtype shallow contact binary with a fill-out factor of f=16.1%and a low mass ratio of q=3.12(or 1/q=0.32).During orbital period investigations,a cyclic variation and a downward parabolic variation with a rate of(-1.28±0.43)×10^(-7) d yr^(-1) was discovered in the observed–calculated(O-C)curve.The cyclic variation was analyzed by the light travel time effect(LTTE)via a potential red dwarf companion star,an orbital semi-major axis shorter than 4.88±0.82 AU was obtained.Finally,we collect physical parameters of a sample of 50 G-type shallow contact binaries(f≤20%),it is suggested that most of the G-type shallow contact binaries are undergoing a longterm and periodic orbital period changes,especially more systems show long-term decreases.The long-term orbital period decrease indicates that IO Cnc is in a mass transferring from the more massive component to the less massive one.With the long-term decrease of the orbital period,this shallow contact binary will evolve into a deeper contact one.展开更多
文摘目的探讨大鼠骨髓间充质干细胞(BMSCs)异体移植对小鼠实验性自身免疫性脑脊髓炎(EAE)的治疗作用。方法全骨髓贴壁培养法获得大鼠BMSCs,流式细胞术检测细胞免疫表型,并诱导成骨方向分化。取雌性C57BL/6小鼠,随机分为3组:正常对照组、PBS组和大鼠BMSCs组,用髓鞘少突胶质细胞糖蛋白(MOG)35~55联合完全弗氏佐剂诱导建立EAE模型。小鼠免疫后38d和48d腹腔注射PBS或大鼠BMSCs进行治疗,神经功能评分观察各组小鼠神经功能变化。二次治疗12d后取各组小鼠脊髓、脾脏和外周血。HE染色及Luxol fast blue染色观察脊髓炎性细胞浸润及髓鞘脱失情况;脾脏制成单细胞悬液,细胞CFSE标记后10mg/L刀豆球蛋白(Con A)和MOG_(35~55)刺激培养3d,观察脾细胞增殖情况。ELISA检测大鼠BMSCs移植后外周血细胞因子干扰素γ(IFN-γ)、白细胞介素17(IL-17)含量。结果流式细胞术显示,大鼠BMSCs第3代(P3)细胞表达抗原CD29、CD90、CD106,不表达CD45。体外诱导其能向成骨分化。小鼠发病后,大鼠BMSCs组小鼠神经功能缺损症状较PBS组减轻,评分降低。HE染色和Luxol fast blue染色结果显示,大鼠BMSCs组脊髓炎细胞浸润和脱髓鞘比同时间点PBS组减轻(P<0.05)。Con A和MOG_(35~55)刺激培养后,PBS组和大鼠BMSCs组脾细胞增殖增加,而大鼠BMSCs组又较PBS组降低。与PBS组相比,大鼠BMSCs组血浆细胞因子IFN-γ、IL-17含量降低(P<0.05)。结论全骨髓贴壁培养法能有效分离纯化BMSCs,大鼠BMSCs异体移植对小鼠EAE模型有治疗作用。
基金supported by the National Natural Science Foundation of China(Grant Nos.11873017 and 11933008)the Science and Technology Talents and Platform Plan of Yunnan province(2018HB070)+1 种基金the Yunnan Natural Science Foundation(No.2018FB006)partially supported by the Open Project Program of the Key Laboratory of Optical Astronomy,National Astronomical Observatories,Chinese Academy of Sciences。
文摘IO Cnc was classified to be a new G-type(G0)W UMa-type eclipsing binary system.Our first multicolor photometric solutions show that IO Cnc is a new W-subtype shallow contact binary with a fill-out factor of f=16.1%and a low mass ratio of q=3.12(or 1/q=0.32).During orbital period investigations,a cyclic variation and a downward parabolic variation with a rate of(-1.28±0.43)×10^(-7) d yr^(-1) was discovered in the observed–calculated(O-C)curve.The cyclic variation was analyzed by the light travel time effect(LTTE)via a potential red dwarf companion star,an orbital semi-major axis shorter than 4.88±0.82 AU was obtained.Finally,we collect physical parameters of a sample of 50 G-type shallow contact binaries(f≤20%),it is suggested that most of the G-type shallow contact binaries are undergoing a longterm and periodic orbital period changes,especially more systems show long-term decreases.The long-term orbital period decrease indicates that IO Cnc is in a mass transferring from the more massive component to the less massive one.With the long-term decrease of the orbital period,this shallow contact binary will evolve into a deeper contact one.
