Objective:Baoyuan decoction(BYD)is a traditional Chinese formula with myocardial protection efficacy validated by modern pharmacological tests.The present study aimed to investigate the effect and mechanism of BYD on ...Objective:Baoyuan decoction(BYD)is a traditional Chinese formula with myocardial protection efficacy validated by modern pharmacological tests.The present study aimed to investigate the effect and mechanism of BYD on alleviating myocardial infarction(MI).Methods:Nuclear magnetic resonance-based serum and urinary metabolomics were employed to explore the metabolic regulation effects of BYD in rats with MI induced by left anterior descending ligation.Oxygen-glucose deprivation/recovery(OGD/R)model in H9c2 cells and multiple molecular biology approaches were used to clarify the underlying action mechanisms of BYD.Results:BYD treatment recovered the serum and urinary metabolite profiles of the MI rats toward normal metabolic status and significantly improved mitochondrial energy metabolism and apoptosis pathways perturbed by MI.Analysis of the molecular mechanism of BYD indicated that it suppressed OGD/R-induced H9c2 cell apoptosis in a concentration-dependent manner by inhibiting the mitochondria-dependent caspase-9/3-poly ADP-ribose polymerase pathway.Conclusions:Our results demonstrate that BYD protects against myocardial apoptosis via the mitochondrial metabolic and apoptosis pathways.They also provide novel insights into the clinical application of BYD for the treatment of ischemic heart diseases.展开更多
海蛇是我国名贵药材之一,新鲜捕捞的海蛇不易保存,一般立刻晒制保存,晒干后海蛇外观变化大,不宜再用外观鉴定,而基因条形码作为一种较新的分子鉴定方法则不再受外观变化困扰,适用于海蛇晒干制品的品种鉴定。该以平颌海蛇Lapemis hardwic...海蛇是我国名贵药材之一,新鲜捕捞的海蛇不易保存,一般立刻晒制保存,晒干后海蛇外观变化大,不宜再用外观鉴定,而基因条形码作为一种较新的分子鉴定方法则不再受外观变化困扰,适用于海蛇晒干制品的品种鉴定。该以平颌海蛇Lapemis hardwickii、环纹海蛇Hydrophis fasciatus、尖尾海蛇Aipysurus eydouxii、贝尔切海蛇H.belcheri和兰伯特海蛇H.lamberti等5种海蛇为研究对象,通过提取实验和基因测序技术测定了这5种海蛇的COI和16S r DNA基因序列,对这些序列进行互相比对并用相似性搜索算法评价5条序列的鉴定成功率。研究显示,16S r DNA基因条形码能初步区分海蛇种属,COI基因条形码序列种内种间差异明显,能进一步区分海蛇品种。因此表明COI序列可以作为海蛇晒干制品的品种鉴定方法。展开更多
基金financially supported by the National Natural Sciences Foundation of China(Nos.81530097 and 81222051)the National Key Technology R&D Program“New Drug Innovation”of China(No.2017ZX09101003-008-003).
文摘Objective:Baoyuan decoction(BYD)is a traditional Chinese formula with myocardial protection efficacy validated by modern pharmacological tests.The present study aimed to investigate the effect and mechanism of BYD on alleviating myocardial infarction(MI).Methods:Nuclear magnetic resonance-based serum and urinary metabolomics were employed to explore the metabolic regulation effects of BYD in rats with MI induced by left anterior descending ligation.Oxygen-glucose deprivation/recovery(OGD/R)model in H9c2 cells and multiple molecular biology approaches were used to clarify the underlying action mechanisms of BYD.Results:BYD treatment recovered the serum and urinary metabolite profiles of the MI rats toward normal metabolic status and significantly improved mitochondrial energy metabolism and apoptosis pathways perturbed by MI.Analysis of the molecular mechanism of BYD indicated that it suppressed OGD/R-induced H9c2 cell apoptosis in a concentration-dependent manner by inhibiting the mitochondria-dependent caspase-9/3-poly ADP-ribose polymerase pathway.Conclusions:Our results demonstrate that BYD protects against myocardial apoptosis via the mitochondrial metabolic and apoptosis pathways.They also provide novel insights into the clinical application of BYD for the treatment of ischemic heart diseases.
文摘海蛇是我国名贵药材之一,新鲜捕捞的海蛇不易保存,一般立刻晒制保存,晒干后海蛇外观变化大,不宜再用外观鉴定,而基因条形码作为一种较新的分子鉴定方法则不再受外观变化困扰,适用于海蛇晒干制品的品种鉴定。该以平颌海蛇Lapemis hardwickii、环纹海蛇Hydrophis fasciatus、尖尾海蛇Aipysurus eydouxii、贝尔切海蛇H.belcheri和兰伯特海蛇H.lamberti等5种海蛇为研究对象,通过提取实验和基因测序技术测定了这5种海蛇的COI和16S r DNA基因序列,对这些序列进行互相比对并用相似性搜索算法评价5条序列的鉴定成功率。研究显示,16S r DNA基因条形码能初步区分海蛇种属,COI基因条形码序列种内种间差异明显,能进一步区分海蛇品种。因此表明COI序列可以作为海蛇晒干制品的品种鉴定方法。
