背景与目的已有的研究结果表明:细胞因子信号通路抑制因子3(suppressor of cytokine signaling 3,SOCS3)能够抑制细胞过度生长、诱导凋亡,具有维持细胞稳定的作用。本研究的目的旨在探讨SOCS3在非小细胞肺癌(NSCLC)组织中的表达,及其与...背景与目的已有的研究结果表明:细胞因子信号通路抑制因子3(suppressor of cytokine signaling 3,SOCS3)能够抑制细胞过度生长、诱导凋亡,具有维持细胞稳定的作用。本研究的目的旨在探讨SOCS3在非小细胞肺癌(NSCLC)组织中的表达,及其与淋巴结转移和临床病理生理特征的关系。检测NSCLC组织中SOCS3基因甲基化状态,分析SOCS3表达与基因甲基化的关系。方法应用Western blotting方法检测30例NSCLC和癌旁肺组织中SOCS3的表达。应用甲基化特异性PCR(MSP)方法检测SOCS3基因甲基化状态。采用免疫组化SP法,检测90例NSCLC组织中SOCS3的表达,分析其与淋巴结转移和临床病理参数的关系。结果30例NSCLC组织中SOCS3的表达显著低于癌旁肺组织(平均灰度值分别为26.3±12.3和78.4±14.5,P=0.000)。NSCLC组织中SOCS3基因甲基化阳性率为(20/30)66.7%,癌旁肺组织为(4/30)13.3%,SOCS3表达下调或缺失与基因异常甲基化相关(r=0.454,P=0.012)。90例癌旁肺组织上皮细胞均有SOCS3表达,NSCLC组织中SOCS3阳性表达率为(41/90)45.6%,淋巴结转移组SOCS3阳性表达率(35.4%)低于无淋巴结转移组(57.1%,P=0.039),Ⅲ期NSCLC中SOCS3阳性表达率(36.4%)低于I+Ⅱ期(60.0%,P=0.028)。SOCS3的表达与组织学类型和分化程度等临床病理参数无关(P>0.05)。结论基因异常甲基化导致SOCS3在NSCLC组织中表达下调,并与NSCLC淋巴结转移和临床病理分期相关。展开更多
侵袭和转移是恶性肿瘤的重要生物学行为之一,淋巴道转移是恶性肿瘤难以根治以及高病死率的主要原因,也是判断患者预后的主要指标。果蝇prospero同源异形盒蛋白1(prospero homeobox protein 1,Prox-1)在胚胎时期淋巴管形成过程中具有重...侵袭和转移是恶性肿瘤的重要生物学行为之一,淋巴道转移是恶性肿瘤难以根治以及高病死率的主要原因,也是判断患者预后的主要指标。果蝇prospero同源异形盒蛋白1(prospero homeobox protein 1,Prox-1)在胚胎时期淋巴管形成过程中具有重要作用,可以作为淋巴管内皮细胞标志物,显示肿瘤新生淋巴管,并与肿瘤淋巴道转移密切相关。尽管Prox-1促进胚胎淋巴管形成机制的研究较为深入,但是在肿瘤淋巴管新生和淋巴道转移的作用机制方面仍有待于进一步探讨,Prox-1可能作为抑制肿瘤淋巴管新生和治疗肿瘤的新靶点,用于阻断肿瘤转移和改善患者预后。现就Prox-1与胚胎淋巴管形成、肿瘤淋巴管新生以及与肿瘤淋巴道转移关系的相关研究现状综述如下。展开更多
Objective： To explore the expression and significance of heparanase in non-small cell lung cancer （NSCLC） regarding prognosis and clinicopathological parameters. Methods： The expression of heparanase was assessed ...Objective： To explore the expression and significance of heparanase in non-small cell lung cancer （NSCLC） regarding prognosis and clinicopathological parameters. Methods： The expression of heparanase was assessed using immunohistochemistry staining and Western blot in 122 paraffin-embedded specimens and 38 freshly taken tissues. The relationship between heparanase expression and the clinicopathological factors was analyzed by Chi-square test, multivariate analysis and Kaplan-Meier method. Results： In the immunoreactive cells, staining was mainly located in cytoplasma and membrane. Human heparanase was highly expressed in lung cancer tissue （78.7%, 96/122） while negative in epithelia of normal lung tissues. The level of heparanase was remarkably higher in NSCLC than that in normal tissue （P=0.043）. Expression of heparanase significantly correlated with TNM stage （P=0.025）, lymphatic metastasis （P=0.002） and vascular invasion （P=0.0003）. The patients with positive heparanase expression had a significantly shorter survival than those with negative heparanase expression （P=0.0006）. In multivariate analysis, only p-TNM stage, lymphatic metastasis and vascular invasion could be considered as prognostic factors. Conclusion： Elevated level of heparanase in human non-small cell lung cancer tissues correlates with the TNM stage, invasion, metastasis and prognosis. However, heparanase expression is not an independent prognostic factor.展开更多
文摘侵袭和转移是恶性肿瘤的重要生物学行为之一,淋巴道转移是恶性肿瘤难以根治以及高病死率的主要原因,也是判断患者预后的主要指标。果蝇prospero同源异形盒蛋白1(prospero homeobox protein 1,Prox-1)在胚胎时期淋巴管形成过程中具有重要作用,可以作为淋巴管内皮细胞标志物,显示肿瘤新生淋巴管,并与肿瘤淋巴道转移密切相关。尽管Prox-1促进胚胎淋巴管形成机制的研究较为深入,但是在肿瘤淋巴管新生和淋巴道转移的作用机制方面仍有待于进一步探讨,Prox-1可能作为抑制肿瘤淋巴管新生和治疗肿瘤的新靶点,用于阻断肿瘤转移和改善患者预后。现就Prox-1与胚胎淋巴管形成、肿瘤淋巴管新生以及与肿瘤淋巴道转移关系的相关研究现状综述如下。
基金This work was supported by the National Natural Sciences Foundation of China (No. 30170409) and the Scientific Research Foundation of Liaoning Education Office (No. 20122178).
文摘Objective： To explore the expression and significance of heparanase in non-small cell lung cancer （NSCLC） regarding prognosis and clinicopathological parameters. Methods： The expression of heparanase was assessed using immunohistochemistry staining and Western blot in 122 paraffin-embedded specimens and 38 freshly taken tissues. The relationship between heparanase expression and the clinicopathological factors was analyzed by Chi-square test, multivariate analysis and Kaplan-Meier method. Results： In the immunoreactive cells, staining was mainly located in cytoplasma and membrane. Human heparanase was highly expressed in lung cancer tissue （78.7%, 96/122） while negative in epithelia of normal lung tissues. The level of heparanase was remarkably higher in NSCLC than that in normal tissue （P=0.043）. Expression of heparanase significantly correlated with TNM stage （P=0.025）, lymphatic metastasis （P=0.002） and vascular invasion （P=0.0003）. The patients with positive heparanase expression had a significantly shorter survival than those with negative heparanase expression （P=0.0006）. In multivariate analysis, only p-TNM stage, lymphatic metastasis and vascular invasion could be considered as prognostic factors. Conclusion： Elevated level of heparanase in human non-small cell lung cancer tissues correlates with the TNM stage, invasion, metastasis and prognosis. However, heparanase expression is not an independent prognostic factor.
