It is reported that 1,25-dihydroxyvitamin D<sub>3</sub>（1,25（OH）<sub>2</sub>D<sub>3</sub>）, a physiological factor, has aninductive effect on the differentiation of a novel huma...It is reported that 1,25-dihydroxyvitamin D<sub>3</sub>（1,25（OH）<sub>2</sub>D<sub>3</sub>）, a physiological factor, has aninductive effect on the differentiation of a novel human megakaryoblastic leukemia cell line（HIMeg） in vitro. At the concentrations ranging from 10<sup>-9</sup> to 10<sup>-6</sup> mol/L, 1,25（OH）<sub>2</sub>D<sub>3</sub> showedinhibition of proliferation on HIMeg cells which was demonstrated by count of survivalcells and cloning efficiency. Meanwhile, using light/electron microscopy, stain of cytochem-istry （including immunoenzymatic technique） and flow cytometry, we found that HIMeg cellscould be further induced into more mature cells in megakaryocytic lineage confirmed by aseries of evidence, including the changes of cell morphology/structure and cytochemistry,increased expression of differentiation antigens on the cell surface, and polyploidization.So, it is possible for 1,25（OH）<sub>2</sub>D<sub>3</sub> to promote the differentiation of the cells in megakaryo-cytic lineage in vivo and to be used to treat acute megakaryoblastic leukemia and other di-seases with展开更多
An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, ...An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".展开更多
文摘It is reported that 1,25-dihydroxyvitamin D<sub>3</sub>（1,25（OH）<sub>2</sub>D<sub>3</sub>）, a physiological factor, has aninductive effect on the differentiation of a novel human megakaryoblastic leukemia cell line（HIMeg） in vitro. At the concentrations ranging from 10<sup>-9</sup> to 10<sup>-6</sup> mol/L, 1,25（OH）<sub>2</sub>D<sub>3</sub> showedinhibition of proliferation on HIMeg cells which was demonstrated by count of survivalcells and cloning efficiency. Meanwhile, using light/electron microscopy, stain of cytochem-istry （including immunoenzymatic technique） and flow cytometry, we found that HIMeg cellscould be further induced into more mature cells in megakaryocytic lineage confirmed by aseries of evidence, including the changes of cell morphology/structure and cytochemistry,increased expression of differentiation antigens on the cell surface, and polyploidization.So, it is possible for 1,25（OH）<sub>2</sub>D<sub>3</sub> to promote the differentiation of the cells in megakaryo-cytic lineage in vivo and to be used to treat acute megakaryoblastic leukemia and other di-seases with
基金the National Natural Science Foundation of China.
文摘An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".
