香蕉是对低温非常敏感的水果。为探索安全、有效防控香蕉采后冷害的方法,以巴西香蕉为试材,分别采用0℃p H 2.4电生酸性功能水和p H 11.3的电生碱性功能水冷激处理30 min(以蒸馏水冷激处理为对照),于(4±1)℃、相对湿度85%条件下贮...香蕉是对低温非常敏感的水果。为探索安全、有效防控香蕉采后冷害的方法,以巴西香蕉为试材,分别采用0℃p H 2.4电生酸性功能水和p H 11.3的电生碱性功能水冷激处理30 min(以蒸馏水冷激处理为对照),于(4±1)℃、相对湿度85%条件下贮藏16 d,研究电生功能水冷激处理对香蕉耐冷性的影响。结果表明:与对照组相比,在(4±1)℃条件下贮藏16 d时,酸性和碱性功能水冷激处理使香蕉的冷害指数分别降低了12%、7%(P<0.05);可显著提高果实硬度和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,降低细胞膜透性,减少丙二醛(MDA)和H2O2的积累量,其中酸性功能水处理组效果更为显著。表明电生功能水冷激处理在一定程度上可提高低温胁迫下香蕉的耐冷性,减少冷害的发生。展开更多
Gp96, a member of HSP90 family, is a versatile molecular chaperone with various newly-discovered functions, for example to serve as a low affinity, high capacity calcium binding protein, a natural adjuvant for therape...Gp96, a member of HSP90 family, is a versatile molecular chaperone with various newly-discovered functions, for example to serve as a low affinity, high capacity calcium binding protein, a natural adjuvant for therapeutic cancer vaccines, a tumor rejection antigen, an immune regulator to pathological cell death. Its multi-functional and structural characteristics make it also an interesting target to develop antibody-based therapeutics. However, its low immunogenicity to mice, because of its high-sequence similarity among different species, is an obstacle to obtain valuable monoclonal antibodies (MAbs). This is a common problem for any low immunogenic proteins, whose sequences share close identity between mice and other species. Here, a new strategy of priming was employed by swine endogenous full-length gp96 and then boosting by E. coli-system heterologously expressed gp96 N-terminal fragment (N-355) to generate MAbs. Twelve different highly-specific MAbs against swine/human endogenous gp96 were successfully obtained. The binding activities of these MAbs were confirmed by enzyme-linked immunosorbent assay (ELISA), Western blot (WB), immunofluorescence and flow cytometry analysis. This provides some important reagents for further research and potential therapeutics. The methods employed can be used for MAb production of any sequence-highly-conserved proteins between mice and swine/human (or any other species).展开更多
文摘香蕉是对低温非常敏感的水果。为探索安全、有效防控香蕉采后冷害的方法,以巴西香蕉为试材,分别采用0℃p H 2.4电生酸性功能水和p H 11.3的电生碱性功能水冷激处理30 min(以蒸馏水冷激处理为对照),于(4±1)℃、相对湿度85%条件下贮藏16 d,研究电生功能水冷激处理对香蕉耐冷性的影响。结果表明:与对照组相比,在(4±1)℃条件下贮藏16 d时,酸性和碱性功能水冷激处理使香蕉的冷害指数分别降低了12%、7%(P<0.05);可显著提高果实硬度和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,降低细胞膜透性,减少丙二醛(MDA)和H2O2的积累量,其中酸性功能水处理组效果更为显著。表明电生功能水冷激处理在一定程度上可提高低温胁迫下香蕉的耐冷性,减少冷害的发生。
基金Project(31030030) supported by the National Natural Science Foundation of China
文摘Gp96, a member of HSP90 family, is a versatile molecular chaperone with various newly-discovered functions, for example to serve as a low affinity, high capacity calcium binding protein, a natural adjuvant for therapeutic cancer vaccines, a tumor rejection antigen, an immune regulator to pathological cell death. Its multi-functional and structural characteristics make it also an interesting target to develop antibody-based therapeutics. However, its low immunogenicity to mice, because of its high-sequence similarity among different species, is an obstacle to obtain valuable monoclonal antibodies (MAbs). This is a common problem for any low immunogenic proteins, whose sequences share close identity between mice and other species. Here, a new strategy of priming was employed by swine endogenous full-length gp96 and then boosting by E. coli-system heterologously expressed gp96 N-terminal fragment (N-355) to generate MAbs. Twelve different highly-specific MAbs against swine/human endogenous gp96 were successfully obtained. The binding activities of these MAbs were confirmed by enzyme-linked immunosorbent assay (ELISA), Western blot (WB), immunofluorescence and flow cytometry analysis. This provides some important reagents for further research and potential therapeutics. The methods employed can be used for MAb production of any sequence-highly-conserved proteins between mice and swine/human (or any other species).
