为探讨富媒体导课(Rich-media Based Lead-in,RBL)教学法在临床医学专业人体寄生虫学教学中的应用效果,选取北京协和医学院2017级临床医学专业的86名学生作为研究对象,将RBL教学法应用于人体寄生虫学课程部分章节教学中,通过问卷调查评...为探讨富媒体导课(Rich-media Based Lead-in,RBL)教学法在临床医学专业人体寄生虫学教学中的应用效果,选取北京协和医学院2017级临床医学专业的86名学生作为研究对象,将RBL教学法应用于人体寄生虫学课程部分章节教学中,通过问卷调查评价教学效果。学生的反馈结果显示,RBL教学法可提升学生对人体寄生虫学课程的兴趣,促进学生对所学内容的理解与掌握,有助于鼓励学生主动学习与独立思考,从而提高课堂教学效率和教学质量。结果表明,RBL教学法有助于提高课堂教学效率和教学质量,值得深入研究与推广。展开更多
Objective Four B and Th cell epitopes were selected from conservative domain of Plasmodium falciparum antigens to construct two groups of chimeric malaria DNA vaccines with different configurations and their antigeni...Objective Four B and Th cell epitopes were selected from conservative domain of Plasmodium falciparum antigens to construct two groups of chimeric malaria DNA vaccines with different configurations and their antigenicities were studied Methods The partially synthesized oligonucleotide was annealed, PCR amplified and cloned into a mammalian cell expression vector By using a pair of isocaudamers on the vector, different single copies of B epitopes were multiplied and were tenderly stringed into two groups of chimeric DNA vaccine with different configurations BALB/c mice were immunized with these DNA plasmids by either intramuscular or intradermal injections Results The antisera from the immunized mice tested by ELISA showed that only the configuration which had a single copy of universal T helper cell epitope, CS T3, located at the C terminal of the multi copy B cell epitopes induced a high antibody response The T helper cell epitope at any other position of the peptide, or the double T helper cell epitopes configured with the B cell epitopes did not enhance antibody response, and some configurations even decreased the humoral response to a B cell epitope Conclusion This study demonstrated that both combination and configuration of the epitope may affect the antigenicity of a chimeric multiple antigen展开更多
文摘为探讨富媒体导课(Rich-media Based Lead-in,RBL)教学法在临床医学专业人体寄生虫学教学中的应用效果,选取北京协和医学院2017级临床医学专业的86名学生作为研究对象,将RBL教学法应用于人体寄生虫学课程部分章节教学中,通过问卷调查评价教学效果。学生的反馈结果显示,RBL教学法可提升学生对人体寄生虫学课程的兴趣,促进学生对所学内容的理解与掌握,有助于鼓励学生主动学习与独立思考,从而提高课堂教学效率和教学质量。结果表明,RBL教学法有助于提高课堂教学效率和教学质量,值得深入研究与推广。
文摘Objective Four B and Th cell epitopes were selected from conservative domain of Plasmodium falciparum antigens to construct two groups of chimeric malaria DNA vaccines with different configurations and their antigenicities were studied Methods The partially synthesized oligonucleotide was annealed, PCR amplified and cloned into a mammalian cell expression vector By using a pair of isocaudamers on the vector, different single copies of B epitopes were multiplied and were tenderly stringed into two groups of chimeric DNA vaccine with different configurations BALB/c mice were immunized with these DNA plasmids by either intramuscular or intradermal injections Results The antisera from the immunized mice tested by ELISA showed that only the configuration which had a single copy of universal T helper cell epitope, CS T3, located at the C terminal of the multi copy B cell epitopes induced a high antibody response The T helper cell epitope at any other position of the peptide, or the double T helper cell epitopes configured with the B cell epitopes did not enhance antibody response, and some configurations even decreased the humoral response to a B cell epitope Conclusion This study demonstrated that both combination and configuration of the epitope may affect the antigenicity of a chimeric multiple antigen
