Objective To explore and develop a Chinese Miniswine model of myocardial infarction (MI) with balloon occlusion of coronary artery. Methods After anesthesia, 20 Chinese Miniswines were subjected to 20 mm or 25 mm bal...Objective To explore and develop a Chinese Miniswine model of myocardial infarction (MI) with balloon occlusion of coronary artery. Methods After anesthesia, 20 Chinese Miniswines were subjected to 20 mm or 25 mm balloon occlusion of the distal part of the second diagnonal branch of left anterior descending (LAD) through femoral artery or carotid artery for the purpose of precondition with 3-4 times of myocardial ischemia. Then, inflated balloon occluded LAD for 120 min. Electrocardiography and blood pressure were monitored. Echocardiography, LDH, CK, CK-MB and coronary angiography were also investigated to confirm acute myocardial infarction (AMI). All pigs were bred for 8 weeks. Results Eighteen out of twenty pigs underwent successful induction of AMI, but one pig died 20 days after operation, two pigs died of ventricular fibrillation during 60-100 rain after operation. AMI was confirmed by dynamic changes of electrocardiography , LDH,CK,CK-MB and further pathology. Regional wall motion abnormalities were found in areas of the superior part of interventricular septum and cardiac apex 1 h after operation by two dimensional echocardiography, repeated echocardiography 3-5 weeks after operation showed wall motion abnormalities in the areas of anterior left ventricular wall, cardiac apex and interventricular septum. Old myocardial infarction (OMI) was confirmed in 17 survival pigs after operation. Conclusions A minimally-invasive swine model of AMI and OMI by using angioplasty catheterization balloon technique is feasible and relatively effective.展开更多
文摘Objective To explore and develop a Chinese Miniswine model of myocardial infarction (MI) with balloon occlusion of coronary artery. Methods After anesthesia, 20 Chinese Miniswines were subjected to 20 mm or 25 mm balloon occlusion of the distal part of the second diagnonal branch of left anterior descending (LAD) through femoral artery or carotid artery for the purpose of precondition with 3-4 times of myocardial ischemia. Then, inflated balloon occluded LAD for 120 min. Electrocardiography and blood pressure were monitored. Echocardiography, LDH, CK, CK-MB and coronary angiography were also investigated to confirm acute myocardial infarction (AMI). All pigs were bred for 8 weeks. Results Eighteen out of twenty pigs underwent successful induction of AMI, but one pig died 20 days after operation, two pigs died of ventricular fibrillation during 60-100 rain after operation. AMI was confirmed by dynamic changes of electrocardiography , LDH,CK,CK-MB and further pathology. Regional wall motion abnormalities were found in areas of the superior part of interventricular septum and cardiac apex 1 h after operation by two dimensional echocardiography, repeated echocardiography 3-5 weeks after operation showed wall motion abnormalities in the areas of anterior left ventricular wall, cardiac apex and interventricular septum. Old myocardial infarction (OMI) was confirmed in 17 survival pigs after operation. Conclusions A minimally-invasive swine model of AMI and OMI by using angioplasty catheterization balloon technique is feasible and relatively effective.
