Cellular morphology was quantitatively analysed by using the point-counting technique In 76 specimens inciuding 18 normal nasopharyngeal epithelia, 16 simple hyperplasia and metaplasia, 18 dysplasia and 24 untreated n...Cellular morphology was quantitatively analysed by using the point-counting technique In 76 specimens inciuding 18 normal nasopharyngeal epithelia, 16 simple hyperplasia and metaplasia, 18 dysplasia and 24 untreated nasopharyngeal carcinoma (NPC). Altogether 19 morphometric parameters of the cell were measured and calculated. THe results showed that with malignant transformation of nasopharyngeal epithelium, the great majority of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal nasopharyngeal epithelium and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasla changed significantly as compared with those in other three groups. Cytomorphometric analysis seems to give strong support to the concept that dysplasla is among the transition stages of disease process between normal nasopharyngeal epithelium and NPC. 19 parameters were processed with multivarite analysis and the discriminatory functions were established with entrance of six parameters. When replaced with the cytomorphometric data obtained from all the 76 cases the discriminatory functions gave a result in accordance with 91 percent of those provided by histopathology.展开更多
Cellular morphology and nuclear DNA content were measured with point-counting technique and flow cytometry (FCM) respectively in normal nasopharyngeal epithelium (NE), tissue adjacent to carcinoma (ATC) and nasopharyn...Cellular morphology and nuclear DNA content were measured with point-counting technique and flow cytometry (FCM) respectively in normal nasopharyngeal epithelium (NE), tissue adjacent to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). The results showed that all 20 patients with NE show ed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 patients with ATC and 2 (33%) of 6 patients with NE positive to EBVCA-IgA test showed nondiploid. A total of 19 morphometric parameters of the cell were measured and calculated. The results showed that with malignant transformation of NE, most of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal NE and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasia changed significantly as compared with those normal NE, simple hyperplasia or metaplasia and NPC groups. Cytomorphome展开更多
AbstractObjective To establish a reliable assay which can be used clinically to detect the antibody to Epstein Barr virus specific DNase (EBV DNase) for the early diagnosis of nasopharyngeal carcinoma by enzyme ...AbstractObjective To establish a reliable assay which can be used clinically to detect the antibody to Epstein Barr virus specific DNase (EBV DNase) for the early diagnosis of nasopharyngeal carcinoma by enzyme linked immunoelectrotransfer blot technique (EITB). Methods P3HR 1 cells were induced with TPA (12 0 tetradecanoyl phorbol 13 acetate) to extract EBV DNase. The sera from 84 patients with nasopharyngeal carcinoma (NPC), 27 patients with other head and neck tumors, and 31 normal healthy adults were detected for EBV DNase by Western Blot and immunochemical staining. Meanwhile, Department of Otolaryngology, Xiangya Hospital, Hunan Medical University, Changsha 410008, China (Tian YQ, Li ZQ, Xiao JY, Tao ZD and Pen YY) they also underwent EBVCA IgA examination. Results There were four positive bands in the region between 52 and 59 KD in the sera from NPC patients. The positive rate by EITB for NPC patients was 70.24%, while that for both patients with other tumors and normal adults was 0. The positive rates of EBVCA IgA were 73.81%, 18.52% and 6.45%, respectively for the three groups. Conclusions EBV DNase detection with EITB is as sensitive as EBVCA IgA examination, but with higher specificity, in NPC. It also has the advantage of not using any radioactive material. Therefore, it is a simple and useful method for early diagnosis of NPC.展开更多
The unclear DNA content was measured with flow cytomctry (FCM) in 167 specimens of normal nasopharyngeal epithelium (NE), adjacent tissue to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). All 20 patients with NE ...The unclear DNA content was measured with flow cytomctry (FCM) in 167 specimens of normal nasopharyngeal epithelium (NE), adjacent tissue to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). All 20 patients with NE showed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 pa tients with ATC and 2 (33%) of 6 patients with NE, positive to EBVCA-IgA test, showed nondiploid. In addition, cellular proliferation index (PI) of diploid NPC and diploid ATC, though differred nonsignificantly, was significantly increased as compared with that of NE (P<0.01). The rate of cervical metastasis in nondiploid NPC was significantly higher than that in diploid NPC (P<0.025).展开更多
文摘Cellular morphology was quantitatively analysed by using the point-counting technique In 76 specimens inciuding 18 normal nasopharyngeal epithelia, 16 simple hyperplasia and metaplasia, 18 dysplasia and 24 untreated nasopharyngeal carcinoma (NPC). Altogether 19 morphometric parameters of the cell were measured and calculated. THe results showed that with malignant transformation of nasopharyngeal epithelium, the great majority of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal nasopharyngeal epithelium and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasla changed significantly as compared with those in other three groups. Cytomorphometric analysis seems to give strong support to the concept that dysplasla is among the transition stages of disease process between normal nasopharyngeal epithelium and NPC. 19 parameters were processed with multivarite analysis and the discriminatory functions were established with entrance of six parameters. When replaced with the cytomorphometric data obtained from all the 76 cases the discriminatory functions gave a result in accordance with 91 percent of those provided by histopathology.
文摘Cellular morphology and nuclear DNA content were measured with point-counting technique and flow cytometry (FCM) respectively in normal nasopharyngeal epithelium (NE), tissue adjacent to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). The results showed that all 20 patients with NE show ed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 patients with ATC and 2 (33%) of 6 patients with NE positive to EBVCA-IgA test showed nondiploid. A total of 19 morphometric parameters of the cell were measured and calculated. The results showed that with malignant transformation of NE, most of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal NE and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasia changed significantly as compared with those normal NE, simple hyperplasia or metaplasia and NPC groups. Cytomorphome
文摘AbstractObjective To establish a reliable assay which can be used clinically to detect the antibody to Epstein Barr virus specific DNase (EBV DNase) for the early diagnosis of nasopharyngeal carcinoma by enzyme linked immunoelectrotransfer blot technique (EITB). Methods P3HR 1 cells were induced with TPA (12 0 tetradecanoyl phorbol 13 acetate) to extract EBV DNase. The sera from 84 patients with nasopharyngeal carcinoma (NPC), 27 patients with other head and neck tumors, and 31 normal healthy adults were detected for EBV DNase by Western Blot and immunochemical staining. Meanwhile, Department of Otolaryngology, Xiangya Hospital, Hunan Medical University, Changsha 410008, China (Tian YQ, Li ZQ, Xiao JY, Tao ZD and Pen YY) they also underwent EBVCA IgA examination. Results There were four positive bands in the region between 52 and 59 KD in the sera from NPC patients. The positive rate by EITB for NPC patients was 70.24%, while that for both patients with other tumors and normal adults was 0. The positive rates of EBVCA IgA were 73.81%, 18.52% and 6.45%, respectively for the three groups. Conclusions EBV DNase detection with EITB is as sensitive as EBVCA IgA examination, but with higher specificity, in NPC. It also has the advantage of not using any radioactive material. Therefore, it is a simple and useful method for early diagnosis of NPC.
文摘The unclear DNA content was measured with flow cytomctry (FCM) in 167 specimens of normal nasopharyngeal epithelium (NE), adjacent tissue to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). All 20 patients with NE showed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 pa tients with ATC and 2 (33%) of 6 patients with NE, positive to EBVCA-IgA test, showed nondiploid. In addition, cellular proliferation index (PI) of diploid NPC and diploid ATC, though differred nonsignificantly, was significantly increased as compared with that of NE (P<0.01). The rate of cervical metastasis in nondiploid NPC was significantly higher than that in diploid NPC (P<0.025).
