综合护理对妊娠高血压疾病患者产后恢复的影响

目的 研究分析综合护理对妊娠高血压疾病患者产后恢复的影响。方法 选取2020年1月至2023年5月在我院接受治疗的妊娠高血压疾病患者60例,根据抽签法分为对照组和观察组。对照组实施常规护理措施,观察组实施综合护理措施,比较两组护理前后的临床指标、负性情绪、生活质量及不良事件情况。结果 观察组的舒张压、收缩压、24 h尿蛋白水平低于对照组(P<0.05);观察组的负性情绪评分比对照组更低(P<0.05);观察组的生活质量评分高于对照组(P<0.05);观察组不良事件发生率(10%)比对照组(36.67%)低(P<0.05)。结论 综合护理对妊娠高血压疾病患者产后的负性情绪具有明显改善作用,同时降低了不良事件的发生率。

一株纤维素降解菌的分离、鉴定及降解特性初步研究

从长沙宁乡灰汤温泉附近的水样中分离获得1株具有较强纤维素降解能力的菌株,命名为NX1-1,该菌株最适生长温度为40℃,最适生长pH5.0-8.0.菌株形态学以及ITS rDNA和28S rDNA D1/D2序列分析的鉴定结果表明,该菌株属于Aspergillus fumigatus这个种.对该菌株产酶特性进行了初步研究,最适产酶条件为：氮源（NH4）2SO4,接种量10%,40℃,初始pH6.0,培养60-96 h.

嗜中高温嗜酸古菌Ferroplasma thermophilum的培养条件优化

在中高温和较低pH条件下,Ferrroplasma spp.是进行硫化矿生物浸出的重要微生物。Ferroplasma spp.为古菌,无细胞壁,对矿浆浓度、搅拌剪切力以及溶液中的重金属离子等敏感,很难得到高密度的纯培养,给大规模的工业应用带来了一定难度。研究了F.thermophilum摇瓶培养时的最佳生长条件,单因素考察结果表明最适培条件为:温度50oC,初始pH0.5,250mL的摇瓶装液量为50mL,无机氮源(NH4)2SO4。通过正交试验确定了FeSO4·7H2O、酵母粉和蛋白胨最适组合为FeSO4·7H2O40g/L,酵母粉0.3g/L,蛋白胨0.2g/L。优化培养后,F.thermophilum浓度达到了6.3×107个/mL,40g/L的FeSO4·7H2O在72h内全部氧化完全。该结果可为该类古菌的扩大培养以及工业应用提供参考。

产退浆酶优良菌株的选育及酶性质分析

为了获得纺织工业中所需求的高温退浆酶,从枯草堆、农田积肥、下水道等处土样中分离得到1株能向胞外分泌耐高温α-淀粉酶的菌株并对其产酶酶学性质进行研究。研究结果表明:该菌为枯草芽孢杆菌(Bacillus subtilis),初步命名为Bacillus subtilisC1;菌株C1在基础培养基中(pH=7,温度45℃)培养24h左右达到生长稳定期,在68h时,酶活达到最高,为185.6U/mL;此α-淀粉酶最适作用pH值为6,最适温度为70℃;酶的耐热性能较好,在70℃处理1h,仍具有81%的酶活,适合高温退浆的需要。

Screening and characterization ofAcidiphilium sp.PJH and its role in bioleaching

克否定的、容许的自造营养物质的细菌种类 PJH 从在 Dexing 矿取样的酸的矿排水被孤立，江西省，中国。isolate 在 1.0 的一个 pH 范围成长了 ? 6.0，与 pH 和 3.5 和 40 的温度 optima #

Bioleaching of chalcopyrite with Acidianus manzaensis YN25 under contact and non-contact conditions

In order to investigate the contributions of contact and non-contact cells of Acidianus manzaensis(A.manzaensis) YN25 to the bioleaching of chalcopyrite,three experiments were carried out in the modified shake flasks.The redox potential,pH,cell density,copper and iron ions in the solution were monitored,and the morphological feature and chemical composition of the leached residues were analyzed.The highest leaching efficiency of Cu and Fe was reached in the experiment where the A.manzaensis YN25 could contact the surface of the chalcopyrite.There was no precipitation of jarosite in the leached residues of three experiments,but there was elemental sulfur in the leached residues when the cells could not contact the chalcopyrite.From these results,it is apparent that the leaching of the chalcopyrite is the cooperative action of the contact and non-contact A.manzaensis YN25.

A new strain Leptospirillum ferriphilum YTW315 for bioleaching of metal sulfides ores

A new strain named YTW315 was isolated from Dexing area using the double-layer culture technique. The morphological, biochemical and physiological characteristics of YTW315 were studied. Physiological investigation indicates that the strain YTW315 is a strict (obligate) chemolithoautotroph, metabolizing ferrous iron and pyrite. The optimal growth conditions for the strain are 40 ℃ and pH 1.6. A phylogenetic analysis based on 16S rRNA sequences shows that the isolate is clustered to Leptospirillum ferriphilum with 99.8% similarity to Leptospirillum ferriphilum strain Fairview and ATCC 49881. The molar fraction of DNA (G+C) of the isolate is 58.1%. The strain can tolerate high concentration of Fe(Ⅲ) and As(V) (500 mmol/L and 50 mmol/L, respectively). Bioleaching experiment indicates that the strain can oxidize Fe(Ⅱ) efficiently, and after 30 d, 44.56% of copper and 95.31% of iron are extracted from chalcopyrite and pyrite, respectively.

Isolation and characterization of acidophilic bacterium from Dongxiangshan Mine in Xinjiang Province, China

One bioleaching bacterium, named as strain DXS, was isolated from acid mine drainages （AMDs） of Dongxiangshan Mine of Hami, Xinjiang Province, China. The strain DXS is gram-negative and rod-shaped with a size of （0.40±0.05） μm x （1.3±0.5） μm. The optimal temperature and pH for growth are 30 ℃ and pH 2.0, respectively. It can grow autotrophically by using ferrous iron, elemental sulfur and NaS203 as sole energy sources. In the phylogenetic tree, strain DXS has similarity with Acidithiobacillus ferrooxidans type strain ATCC 23270 with 99.57% sequence similarity. The cloning and sequencing of Iro protein gene （iro） and tetrathionate hydrolase gene （tth） reveal that strain DXS is completely identical in iro gene sequence to A. ferrooxidans LY （DQ166841）, and almost identical in tth gene sequene to .4. ferrooxidans （AB259312） （only two nucleotides change）. The bioleaching experiments of marmatite and pyrite reveal that the leached zinc and iron concentrations reach 3.01 g/L and 2.75 g/L, respectively. The strain has a well potential application in industry bioleaching.

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