目的分析多学科协作诊疗(multi-disciplinary treatment,MDT)与以问题为基础的教学法-案例教学法(problem based learning-case based teaching,PBL-CBL)联合教学模式用于淋巴瘤临床带教的效果及对教学质量的影响。方法选取2021年2月—2...目的分析多学科协作诊疗(multi-disciplinary treatment,MDT)与以问题为基础的教学法-案例教学法(problem based learning-case based teaching,PBL-CBL)联合教学模式用于淋巴瘤临床带教的效果及对教学质量的影响。方法选取2021年2月—2022年2月在广州医科大学附属第一医院血液内科接受淋巴瘤临床带教的66名实习生为研究对象,依据不同带教模式分成两组。对照组33名采取常规带教模式,观察组33名采取MDT与PBL-CBL联合教学模式,对比两组实习生的考核成绩、岗位胜任能力、综合能力及对教学质量的评价。结果观察组实习生考核成绩总分(92.69±6.19)分,高于对照组的(83.80±8.21)分,差异有统计学意义(P<0.05)。带教前,两组实习生的岗位胜任能力比较,差异无统计学意义(P>0.05);带教后,两组的岗位胜任能力明显提高,且观察组高于对照组,差异有统计学意义(P<0.05)。带教前,两组实习生的综合能力比较,差异无统计学意义(P>0.05);带教后,两组的综合能力明显更高,且观察组高于对照组,差异有统计学意义(P<0.05)。观察组实习生对于教学质量的评价高于对照组,差异有统计学意义(P<0.05)。结论MDT与PBL-CBL联合教学模式用于淋巴瘤临床带教的效果显著,有助提升其考核成绩、岗位胜任能力及综合能力,促进教学质量进一步提高。展开更多
Objective： HOXB7 gene is a kind of transcription regulator over-expressed in malignant melanoma （MM） cell lines. It can specifically up-regulate the expression of angiogenic factors and tumor growth factors such as...Objective： HOXB7 gene is a kind of transcription regulator over-expressed in malignant melanoma （MM） cell lines. It can specifically up-regulate the expression of angiogenic factors and tumor growth factors such as bFGF, GROa, VEGF and induce angiogenesis in melanoma, resulting in the proliferation and metastasis of tumor cells. We designed and synthesized HOXB7 specific siRNA to study its interfering effect on the expressions of HOXB7 and bFGF genes in melanoma A375 cell line and the biologic characteristics of A375 cells. Methods： Three synthesized siRNA with different sequences were separately transfected into A375 cells by lipofecter 2000. The expression of HOXB7 and bFGF mRNA in transfected cells was detected by RT-PCR 24 and 48 hours after transduction. The expression of bFGF protein in the transfected cells were detected by flowcytometry 48 hours after transfection. MTT assay was used to analyze the cell proliferation rate of siRNA transfected cells. Based on the in vitro experiment results, one effective siRNA sequence was selected for the construction of in vivo siRNA expression vector. Then, a malignant melanoma animal model was established. The siRNA expression plasmid was injected into the tumor foci and its influence on the growth and angiogenesis of tumor was observed. Results： The mRNA expressions of both HOXB7 and bFGF genes in the A375 cells reduced significantly 24 and 48 hour after transfection of siRNA. Expression level of the protein of angiogenic factor bFGF induced by HOXB7 gene in siRNA transfected cells was significantly lower than that in control cells 48 hours after transduction. Cell proliferation was also suppressed in siRNA transfected cells. Two of the three siRNA strands showed prominent interference effect. The in vivo study indicated that the tumor size and the microvessel density in the tumor both reduced after injection of HOXB7siRNA plasmid. Conclusion： Down-regulation of HOXB7 gene expression can effectively reduce the expression of angiogenic factor bFGF and the proliferation of MM cells. Besides, the growth and angiogenesis of MM tumor were also inhibited.展开更多
文摘目的分析多学科协作诊疗(multi-disciplinary treatment,MDT)与以问题为基础的教学法-案例教学法(problem based learning-case based teaching,PBL-CBL)联合教学模式用于淋巴瘤临床带教的效果及对教学质量的影响。方法选取2021年2月—2022年2月在广州医科大学附属第一医院血液内科接受淋巴瘤临床带教的66名实习生为研究对象,依据不同带教模式分成两组。对照组33名采取常规带教模式,观察组33名采取MDT与PBL-CBL联合教学模式,对比两组实习生的考核成绩、岗位胜任能力、综合能力及对教学质量的评价。结果观察组实习生考核成绩总分(92.69±6.19)分,高于对照组的(83.80±8.21)分,差异有统计学意义(P<0.05)。带教前,两组实习生的岗位胜任能力比较,差异无统计学意义(P>0.05);带教后,两组的岗位胜任能力明显提高,且观察组高于对照组,差异有统计学意义(P<0.05)。带教前,两组实习生的综合能力比较,差异无统计学意义(P>0.05);带教后,两组的综合能力明显更高,且观察组高于对照组,差异有统计学意义(P<0.05)。观察组实习生对于教学质量的评价高于对照组,差异有统计学意义(P<0.05)。结论MDT与PBL-CBL联合教学模式用于淋巴瘤临床带教的效果显著,有助提升其考核成绩、岗位胜任能力及综合能力,促进教学质量进一步提高。
基金the grants from the Research Foundation of Science & Technology Bureau of Guangzhou(2004Z2-E0011)the Guangdong Province Natural Science Foundation(5002318)
文摘Objective： HOXB7 gene is a kind of transcription regulator over-expressed in malignant melanoma （MM） cell lines. It can specifically up-regulate the expression of angiogenic factors and tumor growth factors such as bFGF, GROa, VEGF and induce angiogenesis in melanoma, resulting in the proliferation and metastasis of tumor cells. We designed and synthesized HOXB7 specific siRNA to study its interfering effect on the expressions of HOXB7 and bFGF genes in melanoma A375 cell line and the biologic characteristics of A375 cells. Methods： Three synthesized siRNA with different sequences were separately transfected into A375 cells by lipofecter 2000. The expression of HOXB7 and bFGF mRNA in transfected cells was detected by RT-PCR 24 and 48 hours after transduction. The expression of bFGF protein in the transfected cells were detected by flowcytometry 48 hours after transfection. MTT assay was used to analyze the cell proliferation rate of siRNA transfected cells. Based on the in vitro experiment results, one effective siRNA sequence was selected for the construction of in vivo siRNA expression vector. Then, a malignant melanoma animal model was established. The siRNA expression plasmid was injected into the tumor foci and its influence on the growth and angiogenesis of tumor was observed. Results： The mRNA expressions of both HOXB7 and bFGF genes in the A375 cells reduced significantly 24 and 48 hour after transfection of siRNA. Expression level of the protein of angiogenic factor bFGF induced by HOXB7 gene in siRNA transfected cells was significantly lower than that in control cells 48 hours after transduction. Cell proliferation was also suppressed in siRNA transfected cells. Two of the three siRNA strands showed prominent interference effect. The in vivo study indicated that the tumor size and the microvessel density in the tumor both reduced after injection of HOXB7siRNA plasmid. Conclusion： Down-regulation of HOXB7 gene expression can effectively reduce the expression of angiogenic factor bFGF and the proliferation of MM cells. Besides, the growth and angiogenesis of MM tumor were also inhibited.
