试验调查了我国广州、昆明、武汉、北京和哈尔滨5个地区在不同季节 VA 菌根真菌(Endogonaceae)的发生频率以及不同作物根际 VA 菌根真菌的种群密度和丰度。结果表明:在我国南方的广州和武汉,VA 菌根真菌的种类较北方的哈尔滨和北京更为...试验调查了我国广州、昆明、武汉、北京和哈尔滨5个地区在不同季节 VA 菌根真菌(Endogonaceae)的发生频率以及不同作物根际 VA 菌根真菌的种群密度和丰度。结果表明:在我国南方的广州和武汉,VA 菌根真菌的种类较北方的哈尔滨和北京更为丰富。各种真菌在秋季分离到的频率较春季高,此外,在秋季真菌的孢子密度也较大。从作物来看:禾本科作物根际较其他几类作物有更高的孢子密度和种群丰度。在我国较普遍发生的真菌种类有:Glomus mos-seae,G.fasciculatum,G.geosporum,G.constrictum 和 G.claroideum。展开更多
建立和优化重组人源中期因子(recombinant human midkine,rhMK)活性检测方法,并对其方法进行验证。本文采用细胞计数试剂盒-8(cell counting kit-8,cck-8)法检测rhMK作用大鼠膝软骨细胞的促增殖活性,并对方法进行专属性、准确性、精密...建立和优化重组人源中期因子(recombinant human midkine,rhMK)活性检测方法,并对其方法进行验证。本文采用细胞计数试剂盒-8(cell counting kit-8,cck-8)法检测rhMK作用大鼠膝软骨细胞的促增殖活性,并对方法进行专属性、准确性、精密性、线性、耐用性验证。RhMK的缓冲液及重组人源白介素-1受体拮抗剂无明显活性效力,该方法专属性良好;相对活性为50%、75%、100%、125%、150%的样品效价回收率(n=6)统计在80.0%~124.0%之间,各相对效价的相对标准偏差均在20%以内,线性相关系数R^(2)≥0.98,表明方法的准确性、精密性、线性均良好;耐用性相关系数R^(2)≥0.92,量效曲线的最大值与最小值的比值≥1.5,方法的耐用性良好。研究建立的rhMK的生物学活性检测方法,其方法验证良好,为rhMK开发过程中常规样品的生物学活性分析提供了可靠的检测方法。本研究已获得上海南方模式生物科技股份有限公司动物看护与使用委员会批准(批准号:2019-0008-06)。展开更多
The growth of vesicular-arbuscular mycorrhizal(VAM) fungi in pure culture in the absence of host tissue is an important problem which greatly limits the deeper studies on taxonomy, genetics and physiology of this grou...The growth of vesicular-arbuscular mycorrhizal(VAM) fungi in pure culture in the absence of host tissue is an important problem which greatly limits the deeper studies on taxonomy, genetics and physiology of this group of fungi and their application in crop produc-展开更多
Investigations of vesicular-arbuscular mycorrhizal(VAM)associations have generally been concentrated on responses of the host plant to fungal infection. Many studies in the recent decades showed that VAM could greatly...Investigations of vesicular-arbuscular mycorrhizal(VAM)associations have generally been concentrated on responses of the host plant to fungal infection. Many studies in the recent decades showed that VAM could greatly improve crop phosphate absorbance,especially in the poor nutrient soils. It becomes clear that VAM is an important biological source in agricultural practice. However, because of the failure of VAM fungal growth in展开更多
文摘试验调查了我国广州、昆明、武汉、北京和哈尔滨5个地区在不同季节 VA 菌根真菌(Endogonaceae)的发生频率以及不同作物根际 VA 菌根真菌的种群密度和丰度。结果表明:在我国南方的广州和武汉,VA 菌根真菌的种类较北方的哈尔滨和北京更为丰富。各种真菌在秋季分离到的频率较春季高,此外,在秋季真菌的孢子密度也较大。从作物来看:禾本科作物根际较其他几类作物有更高的孢子密度和种群丰度。在我国较普遍发生的真菌种类有:Glomus mos-seae,G.fasciculatum,G.geosporum,G.constrictum 和 G.claroideum。
文摘建立和优化重组人源中期因子(recombinant human midkine,rhMK)活性检测方法,并对其方法进行验证。本文采用细胞计数试剂盒-8(cell counting kit-8,cck-8)法检测rhMK作用大鼠膝软骨细胞的促增殖活性,并对方法进行专属性、准确性、精密性、线性、耐用性验证。RhMK的缓冲液及重组人源白介素-1受体拮抗剂无明显活性效力,该方法专属性良好;相对活性为50%、75%、100%、125%、150%的样品效价回收率(n=6)统计在80.0%~124.0%之间,各相对效价的相对标准偏差均在20%以内,线性相关系数R^(2)≥0.98,表明方法的准确性、精密性、线性均良好;耐用性相关系数R^(2)≥0.92,量效曲线的最大值与最小值的比值≥1.5,方法的耐用性良好。研究建立的rhMK的生物学活性检测方法,其方法验证良好,为rhMK开发过程中常规样品的生物学活性分析提供了可靠的检测方法。本研究已获得上海南方模式生物科技股份有限公司动物看护与使用委员会批准(批准号:2019-0008-06)。
文摘The growth of vesicular-arbuscular mycorrhizal(VAM) fungi in pure culture in the absence of host tissue is an important problem which greatly limits the deeper studies on taxonomy, genetics and physiology of this group of fungi and their application in crop produc-
文摘Investigations of vesicular-arbuscular mycorrhizal(VAM)associations have generally been concentrated on responses of the host plant to fungal infection. Many studies in the recent decades showed that VAM could greatly improve crop phosphate absorbance,especially in the poor nutrient soils. It becomes clear that VAM is an important biological source in agricultural practice. However, because of the failure of VAM fungal growth in
