Cisplatin （CDDP）, a platinum-containing drug, has been widely used in the therapy of many types of cancers today. However, the side effects of CDDP have limited its clinical application. Butaselen （BS）, a TrxR inh...Cisplatin （CDDP）, a platinum-containing drug, has been widely used in the therapy of many types of cancers today. However, the side effects of CDDP have limited its clinical application. Butaselen （BS）, a TrxR inhibitor designed by our group, has shown anti-tumor effects in vivo and in vitro. In this study, we investigated the combinatory effects of BS and CDDP on BEL-7402 cells, LoVo cells, HeLa cells and A549 cells. We observed synergistic effects when BS and CDDP were used in combination, especially in BEL-7402 cells. Apoptosis analysis also revealed that the combination treatment of BS and CDDP （1：1） for 48 h induced higher apoptotic effects in BEL-7402 cells. A further study in BEL-7402 cells showed that the synergistic effect might be mediated by the sharp reduction of ratio of Bcl-2/Bax protein. Taken together, the combination of CDDP and BS might be of great potential for chemotherapy.展开更多
基金The National Natural Science Foundation(Grant No.81372266)National Science and Technology Major Project,People’s Republic of China(Grant No.2011zx09101-001-03)
文摘Cisplatin （CDDP）, a platinum-containing drug, has been widely used in the therapy of many types of cancers today. However, the side effects of CDDP have limited its clinical application. Butaselen （BS）, a TrxR inhibitor designed by our group, has shown anti-tumor effects in vivo and in vitro. In this study, we investigated the combinatory effects of BS and CDDP on BEL-7402 cells, LoVo cells, HeLa cells and A549 cells. We observed synergistic effects when BS and CDDP were used in combination, especially in BEL-7402 cells. Apoptosis analysis also revealed that the combination treatment of BS and CDDP （1：1） for 48 h induced higher apoptotic effects in BEL-7402 cells. A further study in BEL-7402 cells showed that the synergistic effect might be mediated by the sharp reduction of ratio of Bcl-2/Bax protein. Taken together, the combination of CDDP and BS might be of great potential for chemotherapy.
