C/EBP is a sequence-specific DNA-binding protein.In order to identify its distribu-tion,localization and function,immunocytochemical technique(ABC method)was done usinganti-C/EBP polypeptide antibodies 1103~#,425~# in...C/EBP is a sequence-specific DNA-binding protein.In order to identify its distribu-tion,localization and function,immunocytochemical technique(ABC method)was done usinganti-C/EBP polypeptide antibodies 1103~#,425~# in liver specimens from 20 normal adult,5neonatal,6 patients with hepatitis,25 patients with liver cirrhosis,80 patients with hepatocellu-lar carcinoma(40 cases were associated with surrounding nontumorous tissues)and 26 patientswith cholangiocarcinoma(15 cases were associated with surrounding nontumorous tissues).Theresults showed that C/EBP was diffusely distributed in nuclei and cytoplasm of differentiated liv-er cells and very low or undetectable in liver cancer cells.The expression of C/EBP was in pro-portion to differentiated degree of tumor cells,and was obviously weaker than that in surround-ing nontumorous tissues.C/EBP positive staining has also been found in regenerating epithelialcells of bile ductules.The results suggested that C/EBP might play an important role in estab-lishing and maintaining the differentiation of liver cells and might exert an inhibiting effect againsttransformation of liver cells and proliferation of neoplastic tissue.展开更多
C / EBP is a sequence-specific DNA-binding protein. In order to indentify its distribution and localization, immunohistochemical technique (ABC method) was done using anti-C / EBP polypeptide antibodies 1103#, 425# in...C / EBP is a sequence-specific DNA-binding protein. In order to indentify its distribution and localization, immunohistochemical technique (ABC method) was done using anti-C / EBP polypeptide antibodies 1103#, 425# in liver specimens from 20 normal adults, 5 neonates, 6 patients with hepatitis, 25 with liver cirrhosis, 80 with hepatocellular carcinoma (40 cases were associated with surrounding nontumorous tissues) and 26 patients with cholangiocarcinoma (15 cases were associated with surrounding nontumorous tissues). The results showed that C / EBP was diffusely distributed in nuclei and cytoplasm of differentiated liver cells and very low or undetectable in liver cancer cells. The manifestation of C / EBP correlated with degree of differentiation of tumour cells, and was obviously weaker than that in surrounding nontumorous tussues. C / EBP positive staining has also been found in regenerating epithelial cells of bile ductules. The results suggested that C / EBP should play an important role in establishing and maintaining the differentiation of liver cells.展开更多
文摘C/EBP is a sequence-specific DNA-binding protein.In order to identify its distribu-tion,localization and function,immunocytochemical technique(ABC method)was done usinganti-C/EBP polypeptide antibodies 1103~#,425~# in liver specimens from 20 normal adult,5neonatal,6 patients with hepatitis,25 patients with liver cirrhosis,80 patients with hepatocellu-lar carcinoma(40 cases were associated with surrounding nontumorous tissues)and 26 patientswith cholangiocarcinoma(15 cases were associated with surrounding nontumorous tissues).Theresults showed that C/EBP was diffusely distributed in nuclei and cytoplasm of differentiated liv-er cells and very low or undetectable in liver cancer cells.The expression of C/EBP was in pro-portion to differentiated degree of tumor cells,and was obviously weaker than that in surround-ing nontumorous tissues.C/EBP positive staining has also been found in regenerating epithelialcells of bile ductules.The results suggested that C/EBP might play an important role in estab-lishing and maintaining the differentiation of liver cells and might exert an inhibiting effect againsttransformation of liver cells and proliferation of neoplastic tissue.
文摘C / EBP is a sequence-specific DNA-binding protein. In order to indentify its distribution and localization, immunohistochemical technique (ABC method) was done using anti-C / EBP polypeptide antibodies 1103#, 425# in liver specimens from 20 normal adults, 5 neonates, 6 patients with hepatitis, 25 with liver cirrhosis, 80 with hepatocellular carcinoma (40 cases were associated with surrounding nontumorous tissues) and 26 patients with cholangiocarcinoma (15 cases were associated with surrounding nontumorous tissues). The results showed that C / EBP was diffusely distributed in nuclei and cytoplasm of differentiated liver cells and very low or undetectable in liver cancer cells. The manifestation of C / EBP correlated with degree of differentiation of tumour cells, and was obviously weaker than that in surrounding nontumorous tussues. C / EBP positive staining has also been found in regenerating epithelial cells of bile ductules. The results suggested that C / EBP should play an important role in establishing and maintaining the differentiation of liver cells.
