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由G_o期过渡到S期时温度敏感突变株tsAF-8的Pre-rRNA基因的活化 被引量:1
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作者 李春华 张鸿卿 +2 位作者 成汝宣 刘俊兰 薛绍白 《细胞生物学杂志》 CSCD 1991年第4期174-177,2,共4页
用0.5%低血清培液使tsAF-8细胞进入G_o态后,再培养于含正常量(10%)血清的培液中,分别在允许温度(33.5℃)、非允许温度(39.5℃)、放线菌素D(0.03μg/ml)(33.5℃)或丁酸钠(5mmol/L)(33.5℃)的条件下,用~3HTdR放射自显影检测G_o期tsAF-8... 用0.5%低血清培液使tsAF-8细胞进入G_o态后,再培养于含正常量(10%)血清的培液中,分别在允许温度(33.5℃)、非允许温度(39.5℃)、放线菌素D(0.03μg/ml)(33.5℃)或丁酸钠(5mmol/L)(33.5℃)的条件下,用~3HTdR放射自显影检测G_o期tsAF-8细胞进入S期的过程,并用银染核仁组织区(NOR)的方法观察pre-rRNA基因(rDNA)的活化程度。在允许温度时G_o期细胞进入S期,pre-rRNA基因被活化。在非允许温度或低浓度放线菌素D(33.5℃)条件下,G_o期细胞不进入S期,pre-rRNA基因也不活化。5mmol/L丁酸钠(33.5℃)条件下细胞不能进入S期,而pre-rRNA基因被活化,但活化程度较低。 展开更多
关键词 细胞 静止期 分裂期 增殖 基因活化
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UPTAKE AND RETENTION OF HEMOTOPORPHYRIN DERIVATIVE (HPD) IN CONFLUENT AND UNCONFLUENT FORSKIN CELLS
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作者 薛绍白 张鸿卿 +5 位作者 成汝宣 房慧玲 宋平根 李素文 黄琳 刘忠敏 《Chinese Science Bulletin》 SCIE EI CAS 1985年第10期1409-1414,共6页
Using HPD plus light as a method of diagnosis and therapy of tumour has received more and more attention recently and has been applied clinically to some degree. But the mechanism involved has not been well understood... Using HPD plus light as a method of diagnosis and therapy of tumour has received more and more attention recently and has been applied clinically to some degree. But the mechanism involved has not been well understood. The tumour tissue could retain more HPD fluorescence than the normal tissue around it 72h after the patient received HPD injection. The normal proliferating tissue like epidemics would also retain 展开更多
关键词 TUMOUR PROLIFERATING understood RETAIN clinically DIAPHRAGM mitochondria histogram wound RETENTION
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