With small somatotype, gentle temperament, balanced hematological and blood biochemical index, Beagle was ideal for laboratory use. Since 1980, beagle has been introducted into our country. They were successfully bred...With small somatotype, gentle temperament, balanced hematological and blood biochemical index, Beagle was ideal for laboratory use. Since 1980, beagle has been introducted into our country. They were successfully bred and multiplied in Guangdong , Shanghai and Beijing . But the control of heredity, balance of gene frequency, gene diversity and gene differentiation and protection of idioplasm has not been well studied. Falk & Holsinger(1991)point out: we can not protect what were unknowed. For operative preventing the inbreeding diminution , gene segregation and differentiation, the DFP and randomly amplified polymorphic (RAPD) technique were applied to assess the genetic variation and colony heredity structural analysis among the population of three internal beagle centers and Marshell beagle in America. The experimental exponent of the heredity analysis in different colony were from Guangdong, Shanghai, Beijing and Marshell (Amereca), and they were not relative. The experimental exponent of the different generation were from Beijing colony, and they were direct maternal side in different generation, but each individual were no relative in the same generation. The heredity structive relatio on different colony and internal colony were analyzed with Phyltools6.0 and SAS6.12 software. The dendrogram of UPGMA based on Nei’s genetic identity of four colony wre constructed. The result showed: There were average distinguished bands 9.8±1.789 in America colony, 10.2±0.837 in Guangdong colony, 10 8±1 483 in Shanghai colony,11.2±2.280 in Beijing colony,and there were 10.5 average bands in the four colonies. The bands owned by both were 0.204 in America colony, 0.294 in Guangdong colony, 0.370 in Shanghai and 0.267 in Beijing respectively. The similarity coefficient were 0.508—0.557(average 0.526). there were no marked difference in the four different area colony. The dendrogram of UPGMA based on Nei’s genetic distance coefficient was drawn: the gene distance of colony between Guangdong and Shanghai was close, and the gene distance of colony between Beijing and America was close. The heredity differentiation and isolation were analyzed with the formula of coefficient of gene dedifferentiation[g=(dt-ds)/dt. Dt was the average partnership distance of all the individuals. Ds was the average partnership distance in the group.].The result showed: the coefficient of heredity dedifferentiation in Marshell was 0.02, the coefficient of gene dedifferentiation in Guanddongwas 0.02, in Shanghai was 0.02, in Beijing was 0.02. It showed the heredity dedifferentiation and isolation among the closed groups were formed. Because the DFP technique was used on genome DNA intron, the RAPD technique was used on genome DNA exon. The result showed: the average smililarity coefficient in different colony was 0.859—0.898. the smililarity coefficient was 0.859±0.0299 in America colony.0.888±0.0315 in Guangdong, 0.898±0.0261in Shanghai,0.884±0.0330in Beijing respectively. The DFP techque was applied to assess the genetic status in Beijing beagle colony. The result showed: the smililarity coefficient was 0.540±0.091 between F1-F2, 0.493±0.128 betweenF2-F3, 0.481±0.100 betweenF1-F3(average 0.505).and F1-F2>F2-F3> F1-F3 .The smililarity coefficient was 0.577±0.124 in F1,0.550±0 009 in F2,0.469±0.113 in F3. Using Sas Analysis of Variance Procedure, there was marked difference between F1 and F2, but there was marked difference between F2 and F3, between F1 and F3. Using the derivation formula of coefficient of inbreeding, the coefficient of inbreeding was 0.334±0.167 in F1, 0.355±0.111 in F2, 0.227±0.100 in F3. The result indicated that the multiply of the three generation was unfit for the themry of Hardy Weinberg Conclusion: 1.Extreme geography differentiation and isolation have been formed in different laboratory beagle groups; 2.There are abundant genetic diversity in the population. 3. The primary beagle closed populations have formed in China on the base of genetic analyse. 4. Severe genetic diversity in idioplasm has been展开更多
文摘With small somatotype, gentle temperament, balanced hematological and blood biochemical index, Beagle was ideal for laboratory use. Since 1980, beagle has been introducted into our country. They were successfully bred and multiplied in Guangdong , Shanghai and Beijing . But the control of heredity, balance of gene frequency, gene diversity and gene differentiation and protection of idioplasm has not been well studied. Falk & Holsinger(1991)point out: we can not protect what were unknowed. For operative preventing the inbreeding diminution , gene segregation and differentiation, the DFP and randomly amplified polymorphic (RAPD) technique were applied to assess the genetic variation and colony heredity structural analysis among the population of three internal beagle centers and Marshell beagle in America. The experimental exponent of the heredity analysis in different colony were from Guangdong, Shanghai, Beijing and Marshell (Amereca), and they were not relative. The experimental exponent of the different generation were from Beijing colony, and they were direct maternal side in different generation, but each individual were no relative in the same generation. The heredity structive relatio on different colony and internal colony were analyzed with Phyltools6.0 and SAS6.12 software. The dendrogram of UPGMA based on Nei’s genetic identity of four colony wre constructed. The result showed: There were average distinguished bands 9.8±1.789 in America colony, 10.2±0.837 in Guangdong colony, 10 8±1 483 in Shanghai colony,11.2±2.280 in Beijing colony,and there were 10.5 average bands in the four colonies. The bands owned by both were 0.204 in America colony, 0.294 in Guangdong colony, 0.370 in Shanghai and 0.267 in Beijing respectively. The similarity coefficient were 0.508—0.557(average 0.526). there were no marked difference in the four different area colony. The dendrogram of UPGMA based on Nei’s genetic distance coefficient was drawn: the gene distance of colony between Guangdong and Shanghai was close, and the gene distance of colony between Beijing and America was close. The heredity differentiation and isolation were analyzed with the formula of coefficient of gene dedifferentiation[g=(dt-ds)/dt. Dt was the average partnership distance of all the individuals. Ds was the average partnership distance in the group.].The result showed: the coefficient of heredity dedifferentiation in Marshell was 0.02, the coefficient of gene dedifferentiation in Guanddongwas 0.02, in Shanghai was 0.02, in Beijing was 0.02. It showed the heredity dedifferentiation and isolation among the closed groups were formed. Because the DFP technique was used on genome DNA intron, the RAPD technique was used on genome DNA exon. The result showed: the average smililarity coefficient in different colony was 0.859—0.898. the smililarity coefficient was 0.859±0.0299 in America colony.0.888±0.0315 in Guangdong, 0.898±0.0261in Shanghai,0.884±0.0330in Beijing respectively. The DFP techque was applied to assess the genetic status in Beijing beagle colony. The result showed: the smililarity coefficient was 0.540±0.091 between F1-F2, 0.493±0.128 betweenF2-F3, 0.481±0.100 betweenF1-F3(average 0.505).and F1-F2>F2-F3> F1-F3 .The smililarity coefficient was 0.577±0.124 in F1,0.550±0 009 in F2,0.469±0.113 in F3. Using Sas Analysis of Variance Procedure, there was marked difference between F1 and F2, but there was marked difference between F2 and F3, between F1 and F3. Using the derivation formula of coefficient of inbreeding, the coefficient of inbreeding was 0.334±0.167 in F1, 0.355±0.111 in F2, 0.227±0.100 in F3. The result indicated that the multiply of the three generation was unfit for the themry of Hardy Weinberg Conclusion: 1.Extreme geography differentiation and isolation have been formed in different laboratory beagle groups; 2.There are abundant genetic diversity in the population. 3. The primary beagle closed populations have formed in China on the base of genetic analyse. 4. Severe genetic diversity in idioplasm has been
