Three patients with pathologically verified spinal osteomyelitis and another three with metastatic tumors of the spine were investigated. MRI of the spine of four patients show-ed several unusual findings. The preserv...Three patients with pathologically verified spinal osteomyelitis and another three with metastatic tumors of the spine were investigated. MRI of the spine of four patients show-ed several unusual findings. The preservation of intervertehral discs and endplates did not predict accurately the diagnosis of either infections or tumors. The pepper and salt appearing feature may also occur in a metastatic tumor. Plain radiographs. CT. and radioisotope bone scans were less sensitive than MRI to disclose the lesions. In cases of osteomyelitis, the systemic sources of infections were frequently not found. and the responsible microorganisms could not be identified even from the surgical specimens of two patients. However, mycobacterium tuberculosis was found in the surgical specimen of a patient with cervical spinal lesion whose MRI was indistinguishable from a metastatic tumor. Surgicopathological diagnosis was therefore crucial and mandatory in these in stances.展开更多
Objective To hibernate fetal ventral mesencephalic tegmental (VMT) cells from Spraque Dawley rats E 15 for 5 days at 4℃ in either hibernation media (HM) or in conditioned hibernation media (CHM) supplemented with ...Objective To hibernate fetal ventral mesencephalic tegmental (VMT) cells from Spraque Dawley rats E 15 for 5 days at 4℃ in either hibernation media (HM) or in conditioned hibernation media (CHM) supplemented with trophic factors such as epidermal growth factor (EGF 200 ng/ml), basic fibroblast growth factor (bFGF 100 ng/ml), recombinant human brain derived neurotrophic factor (rhBDNF 20 μg/ml), recombinant human glial cell derived neurotrophic factor (rhGDNF 20 μg/ml), fetal calf serum (FCS 9%), or human placental cord serum (HPCS 10%). Methods The percent of cell viability and the density of tyrosine hydroxylase immunoreactive (THir) cells in fetal striatal VMT co culture were determined. Results The viability of fetal striatal cells (0.8± 0.104) was slightly higher than that of fetal VMT cells (0.7±0.072) at 0 time point (F 17,1 =4.677; P= 0.045). After 5 days of hibernation, the viability of fetal VMT cells reduced by 30% (F 7,1 =88.493; P<0.001) in HM. However, THir cell density reduced by more than 90% as compared to the freshly harvested VMT cells (F 7,1 =179.944; P<0.001). CHM with HPCS, bFGF, EGF, BDNF, and GDNF showed higher THir cell density than that of HM or CHM supplemented with FCS (P<0.001). Conclusion Supplementation of appropriate trophic factors for hibernated fetal VMT cells promotes cell viability and the subsequent expression of THir cell density.展开更多
文摘Three patients with pathologically verified spinal osteomyelitis and another three with metastatic tumors of the spine were investigated. MRI of the spine of four patients show-ed several unusual findings. The preservation of intervertehral discs and endplates did not predict accurately the diagnosis of either infections or tumors. The pepper and salt appearing feature may also occur in a metastatic tumor. Plain radiographs. CT. and radioisotope bone scans were less sensitive than MRI to disclose the lesions. In cases of osteomyelitis, the systemic sources of infections were frequently not found. and the responsible microorganisms could not be identified even from the surgical specimens of two patients. However, mycobacterium tuberculosis was found in the surgical specimen of a patient with cervical spinal lesion whose MRI was indistinguishable from a metastatic tumor. Surgicopathological diagnosis was therefore crucial and mandatory in these in stances.
文摘Objective To hibernate fetal ventral mesencephalic tegmental (VMT) cells from Spraque Dawley rats E 15 for 5 days at 4℃ in either hibernation media (HM) or in conditioned hibernation media (CHM) supplemented with trophic factors such as epidermal growth factor (EGF 200 ng/ml), basic fibroblast growth factor (bFGF 100 ng/ml), recombinant human brain derived neurotrophic factor (rhBDNF 20 μg/ml), recombinant human glial cell derived neurotrophic factor (rhGDNF 20 μg/ml), fetal calf serum (FCS 9%), or human placental cord serum (HPCS 10%). Methods The percent of cell viability and the density of tyrosine hydroxylase immunoreactive (THir) cells in fetal striatal VMT co culture were determined. Results The viability of fetal striatal cells (0.8± 0.104) was slightly higher than that of fetal VMT cells (0.7±0.072) at 0 time point (F 17,1 =4.677; P= 0.045). After 5 days of hibernation, the viability of fetal VMT cells reduced by 30% (F 7,1 =88.493; P<0.001) in HM. However, THir cell density reduced by more than 90% as compared to the freshly harvested VMT cells (F 7,1 =179.944; P<0.001). CHM with HPCS, bFGF, EGF, BDNF, and GDNF showed higher THir cell density than that of HM or CHM supplemented with FCS (P<0.001). Conclusion Supplementation of appropriate trophic factors for hibernated fetal VMT cells promotes cell viability and the subsequent expression of THir cell density.
