【目的】SWEET(sugars will eventually be exported transporters)是一类参与植物生长发育多个过程的糖转运蛋白,分析DlSWEET1基因在龙眼不同组织和处理下的表达,探究其在果实糖积累中的功能。【方法】以龙眼松风本果实为材料,克隆DlSW...【目的】SWEET(sugars will eventually be exported transporters)是一类参与植物生长发育多个过程的糖转运蛋白,分析DlSWEET1基因在龙眼不同组织和处理下的表达,探究其在果实糖积累中的功能。【方法】以龙眼松风本果实为材料,克隆DlSWEET1基因,采用实时荧光定量PCR分析DlSWEET1在龙眼不同组织器官的表达以及在激素、冷、热、干旱胁迫下的表达模式。通过亚细胞定位、糖转运活性分析及草莓瞬时转化研究DlSWEET1基因的功能。【结果】DlSWEET1基因开放阅读框(ORF)全长为750 bp,编码249个氨基酸,包含一个PQ-loop保守结构域和蛋白典型保守结构域MtN3_slv。DlSWEET1在龙眼根、茎、叶、果肉等组织中均有不同程度的表达,在叶中的表达量较高,在果肉中的表达量次之,而在茎和根中表达量较低;不同浓度的蔗糖、葡萄糖和果糖处理龙眼叶片后,DlSWEET1在叶片中的表达量均有显著升高;低温、干旱及MeJA(茉莉酸甲酯)处理可显著提高DlSWEET1的表达。农杆菌侵染本氏烟草发现DlSWEET1蛋白定位在细胞膜和细胞核。糖转运活性分析证明DlSWEET1蛋白可以转运葡萄糖、果糖、蔗糖和甘露糖。草莓中瞬时转化DlSWEET1可以显著提升果实中的可溶性糖含量。【结论】瞬时过表达DlSWEET1导致转基因草莓果实的可溶性糖含量增加,为进一步解析DlSWEET1在龙眼果实糖积累中的作用提供理论依据。展开更多
A novel neonicotinoid analogue（C13H19N5O5,3a）had been synthesized,the structure was characterized by elemental analysis,IR and 1H NMR spectra,and the S-（＋）-（E）-configuration was confirmed by single-crystal X-ra...A novel neonicotinoid analogue（C13H19N5O5,3a）had been synthesized,the structure was characterized by elemental analysis,IR and 1H NMR spectra,and the S-（＋）-（E）-configuration was confirmed by single-crystal X-ray diffraction.The crystal belongs to monoclinic,space group P21 with a = 8.7076（17）,b = 8.3211（17）,c = 10.642（2）,β = 92.370（3）o,V = 770.4（3）3,Z = 2,Dc = 1.402 g/cm3,μ = 0.110 mm-1,Mr = 325.33,F（000）= 344,S = 1.027,R = 0.0543 and wR = 0.1229 for 3601 unique reflections with 2919 observed ones（I 2σ（I））.Compound 3a is stabilized by intramolecular hydrogen bonds and intermolecular force.In addition,the structure of compound 3a was optimized by the B3LYP/6-31G（d,p）.DFT/B3LYP optimizations were performed based on X-ray geometries applying 6-31G（d,p）basis set.The optimized structure of compound 3a by the B3LYP/6-31G（d,p）method is more bent than in the crystal.IR spectrum of the solid compound is consistent with the X-ray structure.The HOMO-LUMO gap in 3a（5.3 eV）indicates high kinetic stabilities of compound 3a.The preliminary bioassay test showed that 3a exhibited good activities against Nilaparvata legen,Pseudaletia separate Walker and Aphis medicagini at 500 mg/L.展开更多
文摘【目的】SWEET(sugars will eventually be exported transporters)是一类参与植物生长发育多个过程的糖转运蛋白,分析DlSWEET1基因在龙眼不同组织和处理下的表达,探究其在果实糖积累中的功能。【方法】以龙眼松风本果实为材料,克隆DlSWEET1基因,采用实时荧光定量PCR分析DlSWEET1在龙眼不同组织器官的表达以及在激素、冷、热、干旱胁迫下的表达模式。通过亚细胞定位、糖转运活性分析及草莓瞬时转化研究DlSWEET1基因的功能。【结果】DlSWEET1基因开放阅读框(ORF)全长为750 bp,编码249个氨基酸,包含一个PQ-loop保守结构域和蛋白典型保守结构域MtN3_slv。DlSWEET1在龙眼根、茎、叶、果肉等组织中均有不同程度的表达,在叶中的表达量较高,在果肉中的表达量次之,而在茎和根中表达量较低;不同浓度的蔗糖、葡萄糖和果糖处理龙眼叶片后,DlSWEET1在叶片中的表达量均有显著升高;低温、干旱及MeJA(茉莉酸甲酯)处理可显著提高DlSWEET1的表达。农杆菌侵染本氏烟草发现DlSWEET1蛋白定位在细胞膜和细胞核。糖转运活性分析证明DlSWEET1蛋白可以转运葡萄糖、果糖、蔗糖和甘露糖。草莓中瞬时转化DlSWEET1可以显著提升果实中的可溶性糖含量。【结论】瞬时过表达DlSWEET1导致转基因草莓果实的可溶性糖含量增加,为进一步解析DlSWEET1在龙眼果实糖积累中的作用提供理论依据。
基金supported by the National Natural Science Foundation of China (21042010)the Key Laboratory of Rare Earth Functional Materials of Shanghai (07dz22303)the Key Scientific and Technological Project of Shanghai (09391912100)
文摘A novel neonicotinoid analogue（C13H19N5O5,3a）had been synthesized,the structure was characterized by elemental analysis,IR and 1H NMR spectra,and the S-（＋）-（E）-configuration was confirmed by single-crystal X-ray diffraction.The crystal belongs to monoclinic,space group P21 with a = 8.7076（17）,b = 8.3211（17）,c = 10.642（2）,β = 92.370（3）o,V = 770.4（3）3,Z = 2,Dc = 1.402 g/cm3,μ = 0.110 mm-1,Mr = 325.33,F（000）= 344,S = 1.027,R = 0.0543 and wR = 0.1229 for 3601 unique reflections with 2919 observed ones（I 2σ（I））.Compound 3a is stabilized by intramolecular hydrogen bonds and intermolecular force.In addition,the structure of compound 3a was optimized by the B3LYP/6-31G（d,p）.DFT/B3LYP optimizations were performed based on X-ray geometries applying 6-31G（d,p）basis set.The optimized structure of compound 3a by the B3LYP/6-31G（d,p）method is more bent than in the crystal.IR spectrum of the solid compound is consistent with the X-ray structure.The HOMO-LUMO gap in 3a（5.3 eV）indicates high kinetic stabilities of compound 3a.The preliminary bioassay test showed that 3a exhibited good activities against Nilaparvata legen,Pseudaletia separate Walker and Aphis medicagini at 500 mg/L.
