Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogeno...Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogenous H2 S generation, the promoter sequences of L-cysteine desulfhydrase(LCD), D-cysteine desulfhydrase(DCD1,DCD2), desulfhydrase(DES) and nitrogen fixation synthetase(NFS1, NFS2) were analyzed. They are all found to contain a W-box, a characteristic core binding site for the plant WRKY transcription factors, which have important roles in the plant's responses to numerous stresses by modifying the expression patterns of their target genes. An electrophoretic mobility shift assay indicated that WRKY18 and WRKY60 interacted with the W-box in the promoters of the LCD, DCD1, DCD2, DES and NFS2 genes, whereas WRKY40 bound to the W-box of the NFS1 promoter. The expression levels of the LCD, DES and DCD1 genes were up-regulated, but the DCD2 was downregulated in the plants with WRKY18, WRKY40 or WRKY60 mutations. The plants with double and triple mutations of WRKY18, WRKY40 and WRKY60 had a higher rate of H2 S production during cadmium stress and were more resistant to the cadmium stress than the wild type or single mutants. These results suggest that WRKY transcription factors regulate the H2 S signaling pathway in plants, allowing them to cope with cadmium stress.展开更多
泛素蛋白酶体途径(ubiquitin-proteasome pathway,UPP)通过特异性降解目标蛋白调节细胞内蛋白水平及其功能,参与植物对正常生长发育信号和外界环境刺激的响应。SKP1-CUL1-F-box蛋白复合物是E3泛素连接酶的家族成员之一,该蛋白复合体能...泛素蛋白酶体途径(ubiquitin-proteasome pathway,UPP)通过特异性降解目标蛋白调节细胞内蛋白水平及其功能,参与植物对正常生长发育信号和外界环境刺激的响应。SKP1-CUL1-F-box蛋白复合物是E3泛素连接酶的家族成员之一,该蛋白复合体能够靶向识别并介导目标蛋白质的泛素化降解。作为SCF复合体的底物受体交换因子,CAND1(cullin-associated and neddylation-dissociated protein 1)动态调控SCF复合体的组装,在调控植物种子萌发、光形态建成、根系发育、叶脉模式、花器官发育和植物株型等方面发挥重要作用。本文综述了CAND1蛋白的研究历程及其结构与功能,为今后更深入地开展CAND1在植物生长发育中的功能研究提供参考。展开更多
基金supported by the National Natural Science Foundation of China(31300236,31372085 and31400237)Shanxi Province Science Foundation for Youths(2014021026-1)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2013103)
文摘Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogenous H2 S generation, the promoter sequences of L-cysteine desulfhydrase(LCD), D-cysteine desulfhydrase(DCD1,DCD2), desulfhydrase(DES) and nitrogen fixation synthetase(NFS1, NFS2) were analyzed. They are all found to contain a W-box, a characteristic core binding site for the plant WRKY transcription factors, which have important roles in the plant's responses to numerous stresses by modifying the expression patterns of their target genes. An electrophoretic mobility shift assay indicated that WRKY18 and WRKY60 interacted with the W-box in the promoters of the LCD, DCD1, DCD2, DES and NFS2 genes, whereas WRKY40 bound to the W-box of the NFS1 promoter. The expression levels of the LCD, DES and DCD1 genes were up-regulated, but the DCD2 was downregulated in the plants with WRKY18, WRKY40 or WRKY60 mutations. The plants with double and triple mutations of WRKY18, WRKY40 and WRKY60 had a higher rate of H2 S production during cadmium stress and were more resistant to the cadmium stress than the wild type or single mutants. These results suggest that WRKY transcription factors regulate the H2 S signaling pathway in plants, allowing them to cope with cadmium stress.
文摘泛素蛋白酶体途径(ubiquitin-proteasome pathway,UPP)通过特异性降解目标蛋白调节细胞内蛋白水平及其功能,参与植物对正常生长发育信号和外界环境刺激的响应。SKP1-CUL1-F-box蛋白复合物是E3泛素连接酶的家族成员之一,该蛋白复合体能够靶向识别并介导目标蛋白质的泛素化降解。作为SCF复合体的底物受体交换因子,CAND1(cullin-associated and neddylation-dissociated protein 1)动态调控SCF复合体的组装,在调控植物种子萌发、光形态建成、根系发育、叶脉模式、花器官发育和植物株型等方面发挥重要作用。本文综述了CAND1蛋白的研究历程及其结构与功能,为今后更深入地开展CAND1在植物生长发育中的功能研究提供参考。
