In order to create the Male Sterile Line in tobacco, the anti-sense fragment of HSP70 gene was linked to anther specific expression promoter TA29 and the reconstructed vector was transformed into tobacco by Agrobacter...In order to create the Male Sterile Line in tobacco, the anti-sense fragment of HSP70 gene was linked to anther specific expression promoter TA29 and the reconstructed vector was transformed into tobacco by Agrobacterium mediated transformation, and the transformants were then screened. Gus and spot blotting hybridization analysis of the transformants indicated that anti-sense fragment of HSF70 gene had been integrated into tobacco genome and expressed, thus the male sterile tobacco line was obtained. Microscope observation of anther and pollen showed that pistils of transgenic tobacco were normal, whereas anthers and pollens were fairly abortive in the same transgenic tobacco flower, comparing with pistils and stamens in control plants. The ratio of HSI：＇70 protein before and after heat shock in mitochondrial was found to be 1.39 in control tobacco plants and 1.01 in transgenic tobacco sterile lines. This is suggested that the anti-sense gene fragment of HSP70 can effectively inhibit the expression of HSP70 protein and lead to transgenic male sterility in tobacco flowers. The assay provided a new genetic engineering method for male sterility creation in plants.展开更多
Application of high-speed centrifugation and high-performance liquid chro- matography to separation and purification obtained a new type of natural antibacteri- al substance from Escherichia co/i cell. Against Staphy/...Application of high-speed centrifugation and high-performance liquid chro- matography to separation and purification obtained a new type of natural antibacteri- al substance from Escherichia co/i cell. Against Staphy/ococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus, Pseudomonas, solanacearum the minimal inhibitory concentration （MIC） was 2.5, 5, 0.625 iJg/ml, 0.625 and 1.25 g/ml, the minimum bactericidal concentration （MBC） was 5, 5, 1.25, 2.5, 2.5 g/ml, respectively. Composition identification of chemical method and mass spectrometry shows that the antibacterial active substance alkaloids; and the safety evaluation on the model plant Arabidopsis shows that the antibacterial active substance had no inhibitory and adverse effects on the growth and development of plants. The experimental results show that the antibacterial active substance is a broad-spectrum, high efficiency, safe antibacterial natural product, with the prospect of development and utilization of alternative antibiotics.展开更多
基金Supported by National Natural Science Foundation of China(30970247)Hunan Provincial Science Fund for Distinguished Young Scholars(11JJ1007)~~
文摘In order to create the Male Sterile Line in tobacco, the anti-sense fragment of HSP70 gene was linked to anther specific expression promoter TA29 and the reconstructed vector was transformed into tobacco by Agrobacterium mediated transformation, and the transformants were then screened. Gus and spot blotting hybridization analysis of the transformants indicated that anti-sense fragment of HSF70 gene had been integrated into tobacco genome and expressed, thus the male sterile tobacco line was obtained. Microscope observation of anther and pollen showed that pistils of transgenic tobacco were normal, whereas anthers and pollens were fairly abortive in the same transgenic tobacco flower, comparing with pistils and stamens in control plants. The ratio of HSI：＇70 protein before and after heat shock in mitochondrial was found to be 1.39 in control tobacco plants and 1.01 in transgenic tobacco sterile lines. This is suggested that the anti-sense gene fragment of HSP70 can effectively inhibit the expression of HSP70 protein and lead to transgenic male sterility in tobacco flowers. The assay provided a new genetic engineering method for male sterility creation in plants.
基金Supported by Guangdong Scientific and Technological Bureau(2012B020401012)~~
文摘Application of high-speed centrifugation and high-performance liquid chro- matography to separation and purification obtained a new type of natural antibacteri- al substance from Escherichia co/i cell. Against Staphy/ococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus, Pseudomonas, solanacearum the minimal inhibitory concentration （MIC） was 2.5, 5, 0.625 iJg/ml, 0.625 and 1.25 g/ml, the minimum bactericidal concentration （MBC） was 5, 5, 1.25, 2.5, 2.5 g/ml, respectively. Composition identification of chemical method and mass spectrometry shows that the antibacterial active substance alkaloids; and the safety evaluation on the model plant Arabidopsis shows that the antibacterial active substance had no inhibitory and adverse effects on the growth and development of plants. The experimental results show that the antibacterial active substance is a broad-spectrum, high efficiency, safe antibacterial natural product, with the prospect of development and utilization of alternative antibiotics.
