Using genetic recombinant techniques, the tabtoxin resistant gene (trc gene) was to ligated a pBI121 vector under the CaMV 35S promoter to form the binary vector pBITR. Then the vector was introduced into Agrobacteriu...Using genetic recombinant techniques, the tabtoxin resistant gene (trc gene) was to ligated a pBI121 vector under the CaMV 35S promoter to form the binary vector pBITR. Then the vector was introduced into Agrobacterium tumefaciens by triparental mating. After transforming the tobacco, many transgenic plants were obtained. PCR tests indicated that the trc gene was inserted in the genome DNA of tobacco. Biological activity analysis also demonstrated that the transgenic plant alleviated the symptom caused by the pathogen of tobacco wildfire disease.展开更多
文摘Using genetic recombinant techniques, the tabtoxin resistant gene (trc gene) was to ligated a pBI121 vector under the CaMV 35S promoter to form the binary vector pBITR. Then the vector was introduced into Agrobacterium tumefaciens by triparental mating. After transforming the tobacco, many transgenic plants were obtained. PCR tests indicated that the trc gene was inserted in the genome DNA of tobacco. Biological activity analysis also demonstrated that the transgenic plant alleviated the symptom caused by the pathogen of tobacco wildfire disease.