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紫花苜蓿外源基因共转化植株的再生 被引量:30
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作者 吕德扬 曹学远 +1 位作者 唐顺学 田霞 《中国科学(C辑)》 CSCD 2000年第4期342-348,共7页
高含硫氨基酸蛋白(HNP)基因和发根(rol)基因由发根农杆菌介导转入紫花苜蓿,成功地从子叶毛根组织诱导转基因植株再生.子叶是较理想的转化受体,毛根年龄与紫花苜蓿体细胞胚分化频率呈负相关.共转化植株高产、优质性状的产... 高含硫氨基酸蛋白(HNP)基因和发根(rol)基因由发根农杆菌介导转入紫花苜蓿,成功地从子叶毛根组织诱导转基因植株再生.子叶是较理想的转化受体,毛根年龄与紫花苜蓿体细胞胚分化频率呈负相关.共转化植株高产、优质性状的产生对紫花苜蓿新品种的培育有重要意义. 展开更多
关键词 紫花苜蓿 外源基因 共转化植株再生 饲料牧草
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Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes 被引量:1
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作者 吕德扬 曹学远 +1 位作者 唐顺学 田霞 《Science China(Life Sciences)》 SCIE CAS 2000年第4期387-394,共8页
Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully fr... Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties. 展开更多
关键词 MEDICAGO SALIVA HNP gene ROL GENES co-transformed plant.
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