在模拟人体血液p H条件(p H 7.4,离子强度0.1mol/L),通过荧光猝灭、位点竞争、同步荧光和三维荧光光谱等方法研究了柯里拉京(Cor)与人血清白蛋白(HSA)之间相互作用机制。结果表明:HSA的荧光能被Cor静态猝灭,两者间结合常数为2.79×1...在模拟人体血液p H条件(p H 7.4,离子强度0.1mol/L),通过荧光猝灭、位点竞争、同步荧光和三维荧光光谱等方法研究了柯里拉京(Cor)与人血清白蛋白(HSA)之间相互作用机制。结果表明:HSA的荧光能被Cor静态猝灭,两者间结合常数为2.79×103(298K)、2.22×104(304K)和8.41×104L/mol(310K)。根据Van’t Hoff方程结果显示,Cor与HSA间的作用主要为疏水作用,其作用过程为自发、吸热。基于F?rster能量转移,得知Cor与HSA间结合距离为9.33nm。位点竞争实验指出,Cor优先结合HSA的位点III。三维荧光光谱和同步荧光光谱显示,与Cor作用对HSA构象影响不显著。展开更多
In the design of Hydraulic Manifold Blocks (HMB), dynamic performance of inner pipeline networks usually should be evaluated. To meet the design requirements, dynamic characteristic simulation is often needed. Based o...In the design of Hydraulic Manifold Blocks (HMB), dynamic performance of inner pipeline networks usually should be evaluated. To meet the design requirements, dynamic characteristic simulation is often needed. Based on comprehensive study on the existing simulation methods, a new method combined of Power Bond Graph(PBG) and Computational Fluid Dynamic (CFD) is proposed. In this method, flow field of typical channels inside HMB is analyzed with CFD to obtain the local resistance coefficients. Then, with these coefficients, a new sectional lumped-parameter model including kinetic friction factor is developed using PBG. A typical HMB design example is given and the comparison between the simulation and the experimental results demonstrates the feasibility and effectiveness of the proposed method.展开更多
文摘在模拟人体血液p H条件(p H 7.4,离子强度0.1mol/L),通过荧光猝灭、位点竞争、同步荧光和三维荧光光谱等方法研究了柯里拉京(Cor)与人血清白蛋白(HSA)之间相互作用机制。结果表明:HSA的荧光能被Cor静态猝灭,两者间结合常数为2.79×103(298K)、2.22×104(304K)和8.41×104L/mol(310K)。根据Van’t Hoff方程结果显示,Cor与HSA间的作用主要为疏水作用,其作用过程为自发、吸热。基于F?rster能量转移,得知Cor与HSA间结合距离为9.33nm。位点竞争实验指出,Cor优先结合HSA的位点III。三维荧光光谱和同步荧光光谱显示,与Cor作用对HSA构象影响不显著。
基金National Natural Science Foundation of China (No.50375023)
文摘In the design of Hydraulic Manifold Blocks (HMB), dynamic performance of inner pipeline networks usually should be evaluated. To meet the design requirements, dynamic characteristic simulation is often needed. Based on comprehensive study on the existing simulation methods, a new method combined of Power Bond Graph(PBG) and Computational Fluid Dynamic (CFD) is proposed. In this method, flow field of typical channels inside HMB is analyzed with CFD to obtain the local resistance coefficients. Then, with these coefficients, a new sectional lumped-parameter model including kinetic friction factor is developed using PBG. A typical HMB design example is given and the comparison between the simulation and the experimental results demonstrates the feasibility and effectiveness of the proposed method.
