Objective To investigate the effect of endotoxin and tumor necrosis factor(TNF)on hepatic function of rats.Method Prepare hepatic cell suspension by filling male SD rats’ livers with collagenaseⅠ,culture in DMEM med...Objective To investigate the effect of endotoxin and tumor necrosis factor(TNF)on hepatic function of rats.Method Prepare hepatic cell suspension by filling male SD rats’ livers with collagenaseⅠ,culture in DMEM medium,and add endotoxin and TNF in it.After 24hours detect GPT,GOT in supernatant fluid.Result The activity of GPT and GOT in superna tant fluid increased after adding en dotoxin and TNF .Conclusion The system of hepatic cell membrane c ould be damaged severely after acted by endotoxin and TNF.展开更多
文摘Objective To investigate the effect of endotoxin and tumor necrosis factor(TNF)on hepatic function of rats.Method Prepare hepatic cell suspension by filling male SD rats’ livers with collagenaseⅠ,culture in DMEM medium,and add endotoxin and TNF in it.After 24hours detect GPT,GOT in supernatant fluid.Result The activity of GPT and GOT in superna tant fluid increased after adding en dotoxin and TNF .Conclusion The system of hepatic cell membrane c ould be damaged severely after acted by endotoxin and TNF.