In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(D...In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(DNR) to form SM6:Dex: DNR immunoconjugate.The activity of anti-Id-McAb and DNR of the immunoconjugate and their extent of the substitution were measured,and the autitumor activity of SM6:hex:DNR was assayed in vitro.The results demonstrateil that using Dex-T40 to prepare immunoconjugate could get a higher extent of substitution of McAb and DNR and maintain the activity of McAb and drug well.The results of cytotoxic assay in vitro suggested that the immunoconjugate SM6:Dex:DNR possess a specific cytotoxic effett to target tumor cells.展开更多
文摘In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(DNR) to form SM6:Dex: DNR immunoconjugate.The activity of anti-Id-McAb and DNR of the immunoconjugate and their extent of the substitution were measured,and the autitumor activity of SM6:hex:DNR was assayed in vitro.The results demonstrateil that using Dex-T40 to prepare immunoconjugate could get a higher extent of substitution of McAb and DNR and maintain the activity of McAb and drug well.The results of cytotoxic assay in vitro suggested that the immunoconjugate SM6:Dex:DNR possess a specific cytotoxic effett to target tumor cells.
