Acupuncture can mobilize endogenous opioid system and produce analgesia. However, there is some variability of the analgesic effect between individuals, which is related with cholecystokin level of the subject. In the...Acupuncture can mobilize endogenous opioid system and produce analgesia. However, there is some variability of the analgesic effect between individuals, which is related with cholecystokin level of the subject. In the present study, we try to use functional magnetic resonance imaging (fMRI) to address the question by which neuropathways CCK influences acupuncture induced analgesia. Two different frequencies of transcutaneous electric acupoint stimulation (TEAS) were administered to normal human body. TEAS was used instead of traditional acupuncture for its similar analgesic effect and convenience of parameter adjustment. Our previous studies have indicated that low and high frequency TEAS generated effects through different neuropathways, in which some nuclei of midbrain, thalamus and hypothalamus played an important role. 25 healthy volunteers were randomly divided into two groups to receive low and high frequency TEAS respectively. Data from functional MRI scanning (EPI sequence, TR=3000 ms, TE=45 ms, Flip angle: 90°) were collected at the same time of stimulation. Basal and after TEAS pain thresholds were measured by radiant heat withdrawal test 1～3 days before fMRI examination and changes of pain threshold were calculated as the index of analgesic effect of TEAS. Functional data were processed with cross correlation of timecourse and stimulation curve after registration, normalization and detrending. The averaged signal intensity of every interested region was linear regressed according to the change of pain threshold. We found that in low frequency TEAS group, activation intensity of contralateral primary and supplementary motor areas (MI and SMA), bilateral secondary somatosensory area (SⅡ), contralateral thalamus and anterior cingulate cortex (BA 24), ipsilateral superior temporal gyrus, insula had a significant linear correlation with the change of pain threshold. Furthermore, the signal intensity of bilateral hippocampus and the change of pain threshold had a negative linear correlation. While in the areas of primary somatosensory area (SⅠ) and inferior parietal lobule (BA 40), such linear correlation did not exist. In the case of high frequency TEAS, the results were similar to those mentioned above, that is, the corresponding somatosensory areas and the connective cortex were all activated. While the relevant motor related areas were seldom activated. Parts of the limbic system such as bilateral amygdala, perigenual anterior cingulate cortex, nucleus accumbence, and premedial frontal cortex, were inhibited, among which signals of amygdala and nucleus accumbence were negatively related with the analgesic effect. Our results suggested that different frequencies of TEAS stimulation activated different brain areas. Some brain areas are specifically involved in the TEAS analgesia process. They may play an important role in the acupuncture analgesic neuropathways.展开更多
目的观察模拟失重对脑认知功能的影响及中药的干预效果。方法 16名志愿者,-6°头低位卧床(head-down bed rest,HDBR)3周前和卧床结束当天起床前各进行一次fMRI实验,包括中性、冲突两种任务模式和事件相关设计。随机分对照组(8人)、...目的观察模拟失重对脑认知功能的影响及中药的干预效果。方法 16名志愿者,-6°头低位卧床(head-down bed rest,HDBR)3周前和卧床结束当天起床前各进行一次fMRI实验,包括中性、冲突两种任务模式和事件相关设计。随机分对照组(8人)、用药组(8人),用药组卧床期间服用自制中药制剂200 mL/d,3周,对照组服用等量安慰剂。结果两组模拟失重前:前扣带回、额叶和丘脑出现显著的激活。模拟失重后(对照组):额叶、前扣带回激活区的范围和信号强度较卧床前显著减少,丘脑激活消失。模拟失重后(用药组):前扣带回出现了显著的激活区,丘脑可见较显著的激活区,右侧额叶额中回小范围激活区。脑激活区范围和信号强度接近模拟失重前状态。结论模拟失重状态对认知功能有明显的影响,中药可以增强相关脑区对认知功能的调控作用。展开更多
文摘Acupuncture can mobilize endogenous opioid system and produce analgesia. However, there is some variability of the analgesic effect between individuals, which is related with cholecystokin level of the subject. In the present study, we try to use functional magnetic resonance imaging (fMRI) to address the question by which neuropathways CCK influences acupuncture induced analgesia. Two different frequencies of transcutaneous electric acupoint stimulation (TEAS) were administered to normal human body. TEAS was used instead of traditional acupuncture for its similar analgesic effect and convenience of parameter adjustment. Our previous studies have indicated that low and high frequency TEAS generated effects through different neuropathways, in which some nuclei of midbrain, thalamus and hypothalamus played an important role. 25 healthy volunteers were randomly divided into two groups to receive low and high frequency TEAS respectively. Data from functional MRI scanning (EPI sequence, TR=3000 ms, TE=45 ms, Flip angle: 90°) were collected at the same time of stimulation. Basal and after TEAS pain thresholds were measured by radiant heat withdrawal test 1～3 days before fMRI examination and changes of pain threshold were calculated as the index of analgesic effect of TEAS. Functional data were processed with cross correlation of timecourse and stimulation curve after registration, normalization and detrending. The averaged signal intensity of every interested region was linear regressed according to the change of pain threshold. We found that in low frequency TEAS group, activation intensity of contralateral primary and supplementary motor areas (MI and SMA), bilateral secondary somatosensory area (SⅡ), contralateral thalamus and anterior cingulate cortex (BA 24), ipsilateral superior temporal gyrus, insula had a significant linear correlation with the change of pain threshold. Furthermore, the signal intensity of bilateral hippocampus and the change of pain threshold had a negative linear correlation. While in the areas of primary somatosensory area (SⅠ) and inferior parietal lobule (BA 40), such linear correlation did not exist. In the case of high frequency TEAS, the results were similar to those mentioned above, that is, the corresponding somatosensory areas and the connective cortex were all activated. While the relevant motor related areas were seldom activated. Parts of the limbic system such as bilateral amygdala, perigenual anterior cingulate cortex, nucleus accumbence, and premedial frontal cortex, were inhibited, among which signals of amygdala and nucleus accumbence were negatively related with the analgesic effect. Our results suggested that different frequencies of TEAS stimulation activated different brain areas. Some brain areas are specifically involved in the TEAS analgesia process. They may play an important role in the acupuncture analgesic neuropathways.
文摘目的观察模拟失重对脑认知功能的影响及中药的干预效果。方法 16名志愿者,-6°头低位卧床(head-down bed rest,HDBR)3周前和卧床结束当天起床前各进行一次fMRI实验,包括中性、冲突两种任务模式和事件相关设计。随机分对照组(8人)、用药组(8人),用药组卧床期间服用自制中药制剂200 mL/d,3周,对照组服用等量安慰剂。结果两组模拟失重前:前扣带回、额叶和丘脑出现显著的激活。模拟失重后(对照组):额叶、前扣带回激活区的范围和信号强度较卧床前显著减少,丘脑激活消失。模拟失重后(用药组):前扣带回出现了显著的激活区,丘脑可见较显著的激活区,右侧额叶额中回小范围激活区。脑激活区范围和信号强度接近模拟失重前状态。结论模拟失重状态对认知功能有明显的影响,中药可以增强相关脑区对认知功能的调控作用。
