基于小麦白粉病菌rDNA ITS序列的PCR分子检测

Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat.

新创办英文科技期刊提升国际影响力的重要举措探析——以Phytopathology Research为例

【目的】探讨英文科技期刊初创阶段提高国际影响力的重要举措,为新创办英文科技期刊提供参考。【方法】从邀约稿件、学术质量把控、同行评议进程追踪、出版模式和宣传渠道等角度入手,通过流程展示和数据分析,总结Phytopathology Research创办过程中的经验和不足。【结果】Phytopathology Research在创刊3年内先后被DOAJ、ESCI、Scopus和SCIE收录,从无到有实现跨越式发展,在植物科学领域位列Q1区。【结论】以邀约高质量稿件为主带动自由投稿质量提升、通过编委负责制把控稿件质量、科学追踪管理同行评议进程、高质量编辑加工、全开放获取出版,以及多渠道对外宣传是快速提升新创办英文科技期刊影响力的重要措施。