对不同氧化还原状态的细胞色素 bc_1复合物的共振拉曼光谱进行了分析比较。对部分拉曼信号的变化进行了指认。细胞色素 c_1的还原引起1640cm^(_1)和1560cm^(_1)及1454cm^(_1)的明显变小,细胞色素 b 的还原引起1544cm^(_1)的下降。这些...对不同氧化还原状态的细胞色素 bc_1复合物的共振拉曼光谱进行了分析比较。对部分拉曼信号的变化进行了指认。细胞色素 c_1的还原引起1640cm^(_1)和1560cm^(_1)及1454cm^(_1)的明显变小,细胞色素 b 的还原引起1544cm^(_1)的下降。这些信号可以标志复合物中不同色素的氧化还原状态,对拉曼方法用于研究呼吸链酶系有重要参考价值。展开更多
A simple method to obtain large red crystals of cytochrome bc1 complex from beef heart mitochondria has been developed. These crystals are very stable. Their shapes are retained for a long time in tip-sealed Pasteur p...A simple method to obtain large red crystals of cytochrome bc1 complex from beef heart mitochondria has been developed. These crystals are very stable. Their shapes are retained for a long time in tip-sealed Pasteur pipets placed in a refrigerator. The structure of crystalline cytochrome bc1 complex by micro FT-IR spectroscopy has been investigated. Based on the IR spectra of cytochrome c, the empirical assignments of the major infrared frequencies of cytochrome bc1 complex are given. Infrared frequencies and relative intensities of variable orientation and section of crystal are significantly different. These imply that infrared spectral characterization of the membrane protein crystallization is associated with the variable symmetries and orientations of the structure. Experimental results show that phospholipid exists in the crystal of cytochrome bc1 complex. The membrane protein is probably spanned on the mitochondrial membrane and buried in phospholipid bilayer in an asymmetric manner.展开更多
文摘对不同氧化还原状态的细胞色素 bc_1复合物的共振拉曼光谱进行了分析比较。对部分拉曼信号的变化进行了指认。细胞色素 c_1的还原引起1640cm^(_1)和1560cm^(_1)及1454cm^(_1)的明显变小,细胞色素 b 的还原引起1544cm^(_1)的下降。这些信号可以标志复合物中不同色素的氧化还原状态,对拉曼方法用于研究呼吸链酶系有重要参考价值。
基金Project supported by the National Natural Science Foundation of China.
文摘A simple method to obtain large red crystals of cytochrome bc1 complex from beef heart mitochondria has been developed. These crystals are very stable. Their shapes are retained for a long time in tip-sealed Pasteur pipets placed in a refrigerator. The structure of crystalline cytochrome bc1 complex by micro FT-IR spectroscopy has been investigated. Based on the IR spectra of cytochrome c, the empirical assignments of the major infrared frequencies of cytochrome bc1 complex are given. Infrared frequencies and relative intensities of variable orientation and section of crystal are significantly different. These imply that infrared spectral characterization of the membrane protein crystallization is associated with the variable symmetries and orientations of the structure. Experimental results show that phospholipid exists in the crystal of cytochrome bc1 complex. The membrane protein is probably spanned on the mitochondrial membrane and buried in phospholipid bilayer in an asymmetric manner.
