Objectives:The gray mold fungus Botrytis cinerea(B.cinerea)infects a wide range of crops before and after harvest,causing huge losses worldwide.Inhibition mechanisms of B.cinerea in vitro and in plants by calcium prop...Objectives:The gray mold fungus Botrytis cinerea(B.cinerea)infects a wide range of crops before and after harvest,causing huge losses worldwide.Inhibition mechanisms of B.cinerea in vitro and in plants by calcium propionate(CP),generally recognized as a safe substanee,are described in this study.Materials and methods:Wild-type and transgenic mutant strains of B.cinerea were used in the study to evaluate the effects of CP on fun gal growth and developme nt in vitro.Plant materials in cludi ng tomato leaves and table grapes were tested for con trolling efficie ncy of CP agai nst gray mold deterioration in vivo.Results:Mycelial growth of B.cinerea was inhibited by CP in a dose-dependent manner with occasional disruption of hyphal tips,causing cellular collapse and efflux of cell contents.Staining with fluorescein diacetate and propidium iodide indicated that CP decreased fungal cell viability.Inhibition efficiency of CP against B.cinerea was enhaneed by reducing pH.In contrast,the veil mutant,which exhibited deficiency in acid production,was more resista nt to CP,suggesti ng that inhibition of B.cinerea by CP is enhanced by the acidification ability of the fungus itself.Additionally,CP inhibited infection cushion development by germlings of B.cinerea.Infection assays with tomato leaves and table grapes showed that CP inhibited decay development in both host tissues.Moreover,application of CP on grapes 3 days prior to harvest could contribute to management of deterioration caused by spontaneous fungal diseases during storage.Conclusion:CP can suppress hyphal growth,inhibit infection cushion development,and reduce the virulence of B.cinerea.CP is thus promising for practical management of gray mold in fruit crops and merits further evaluation.展开更多
基金supported by the grants from Shanghai Municipal Science and Technology Commission,China(No.18391901400)the National Key Research and Development Program of China(2016YFD0400105)+1 种基金the National Natural Science Foundation of China(No.32061133006)the Shanghai Municipal Agricultural and Rural Committee(2019-02-08-00-02-F0U46),China。
文摘Objectives:The gray mold fungus Botrytis cinerea(B.cinerea)infects a wide range of crops before and after harvest,causing huge losses worldwide.Inhibition mechanisms of B.cinerea in vitro and in plants by calcium propionate(CP),generally recognized as a safe substanee,are described in this study.Materials and methods:Wild-type and transgenic mutant strains of B.cinerea were used in the study to evaluate the effects of CP on fun gal growth and developme nt in vitro.Plant materials in cludi ng tomato leaves and table grapes were tested for con trolling efficie ncy of CP agai nst gray mold deterioration in vivo.Results:Mycelial growth of B.cinerea was inhibited by CP in a dose-dependent manner with occasional disruption of hyphal tips,causing cellular collapse and efflux of cell contents.Staining with fluorescein diacetate and propidium iodide indicated that CP decreased fungal cell viability.Inhibition efficiency of CP against B.cinerea was enhaneed by reducing pH.In contrast,the veil mutant,which exhibited deficiency in acid production,was more resista nt to CP,suggesti ng that inhibition of B.cinerea by CP is enhanced by the acidification ability of the fungus itself.Additionally,CP inhibited infection cushion development by germlings of B.cinerea.Infection assays with tomato leaves and table grapes showed that CP inhibited decay development in both host tissues.Moreover,application of CP on grapes 3 days prior to harvest could contribute to management of deterioration caused by spontaneous fungal diseases during storage.Conclusion:CP can suppress hyphal growth,inhibit infection cushion development,and reduce the virulence of B.cinerea.CP is thus promising for practical management of gray mold in fruit crops and merits further evaluation.
