1973年,美国斯坦福大学的博耶(H.Boyer)和科恩(S.Cohen)采用 DNA 重组技术,首次获得外源 DNA 的分子克隆,从而开始了在分子水平上对遗传物质的施工和改建,即基因工程。十年之后,厄尔默(K.M.Ul-mer)提出蛋白质工程的设想,即通过对蛋白质...1973年,美国斯坦福大学的博耶(H.Boyer)和科恩(S.Cohen)采用 DNA 重组技术,首次获得外源 DNA 的分子克隆,从而开始了在分子水平上对遗传物质的施工和改建,即基因工程。十年之后,厄尔默(K.M.Ul-mer)提出蛋白质工程的设想,即通过对蛋白质已知晶体结构的了解,借助计算机辅助设计,用基因定位诱变等技术改造基因。展开更多
Hirudin gene was mutated by error\|prone PCR and DNA shuffling.The shuffled genes were inserted into phagemid pCANTAB\|5G8.After the introduction of the recombinant DNA into E.coli TG1,the hirudin mutants were display...Hirudin gene was mutated by error\|prone PCR and DNA shuffling.The shuffled genes were inserted into phagemid pCANTAB\|5G8.After the introduction of the recombinant DNA into E.coli TG1,the hirudin mutants were displayed on the surface of bacteriophage M13.A mutant with increased specific activity of antithrombin was selected by two rounds of panning with decreased amounts of thrombin,and was identified as N47K.Activity analysis of HV2 and its mutants N47K,N26S,D5A,P46L/S50G suggested that K47,P46 were important to the interaction of hirudin and thrombin.Hirudin mutants with improved properties could be hopefully aquired through directed evolution by DNA shuffling.展开更多
文摘1973年,美国斯坦福大学的博耶(H.Boyer)和科恩(S.Cohen)采用 DNA 重组技术,首次获得外源 DNA 的分子克隆,从而开始了在分子水平上对遗传物质的施工和改建,即基因工程。十年之后,厄尔默(K.M.Ul-mer)提出蛋白质工程的设想,即通过对蛋白质已知晶体结构的了解,借助计算机辅助设计,用基因定位诱变等技术改造基因。
文摘Hirudin gene was mutated by error\|prone PCR and DNA shuffling.The shuffled genes were inserted into phagemid pCANTAB\|5G8.After the introduction of the recombinant DNA into E.coli TG1,the hirudin mutants were displayed on the surface of bacteriophage M13.A mutant with increased specific activity of antithrombin was selected by two rounds of panning with decreased amounts of thrombin,and was identified as N47K.Activity analysis of HV2 and its mutants N47K,N26S,D5A,P46L/S50G suggested that K47,P46 were important to the interaction of hirudin and thrombin.Hirudin mutants with improved properties could be hopefully aquired through directed evolution by DNA shuffling.