A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs...A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs) and integrated into a microfluidic channel. By modifying the TCF surface with monolayer poly-l-lysine(PLL) and single-stranded deoxyribonucleic acid(ssDNA) probes, the target DNA molecules can be captured in the microfluidic channel. The transmission spectra of the biosensor are measured and theoretically analyzed under different biosensing reaction processes. The results show that the wavelength has a blue-shift with the process of the DNA hybridization. Due to the advantages of low cost, simple operation as well as good detection effect on DNA molecules hybridization, the proposed biosensor has great application prospects in the fields of gene sequencing, medical diagnosis, cancer detection and environmental engineering.展开更多
文摘为在利用神经网络对地表土壤含水量的模拟中,实现对降水等天然要素和人类活动用水的综合考虑,以MPDI(modified perpendicular drought index)作为人类活动作用下的地表干湿状况指标,结合传统的天然要素构建土壤含水量神经网络模型,对河北省2018年地表土壤含水量进行了模拟。结果表明:考虑MPDI的土壤含水量模拟结果与实测值一致性较好,训练期相关系数为0.7,验证期为0.5;分析了神经网络的土壤含水量结果在单一日期不同站点的空间分布情况,在示例日期的相关系数是0.67,模拟结果能较好地体现土壤含水量的空间异质性;模拟结果与SMAP(soil moisture active and passive)土壤含水量产品在河北省具有较好的一致性,均为夏季高、春季低,东部平原高、西部和北部山地低。
基金supported by the National Undergraduate Innovation Training Program(No.201710055065)
文摘A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs) and integrated into a microfluidic channel. By modifying the TCF surface with monolayer poly-l-lysine(PLL) and single-stranded deoxyribonucleic acid(ssDNA) probes, the target DNA molecules can be captured in the microfluidic channel. The transmission spectra of the biosensor are measured and theoretically analyzed under different biosensing reaction processes. The results show that the wavelength has a blue-shift with the process of the DNA hybridization. Due to the advantages of low cost, simple operation as well as good detection effect on DNA molecules hybridization, the proposed biosensor has great application prospects in the fields of gene sequencing, medical diagnosis, cancer detection and environmental engineering.
