The selectivity of Tetranychus urticae and Tetranychus viennensis to Ginkgo biloba leaves was determined by means of method of petri dish, and elucidated the resistance of G.biloba against spider mites, and then the i...The selectivity of Tetranychus urticae and Tetranychus viennensis to Ginkgo biloba leaves was determined by means of method of petri dish, and elucidated the resistance of G.biloba against spider mites, and then the influence of volatiles from G.bioloba leaves on selection behavior of spider mites was explored.Volatiles from G.biloba leaves which were collected with solid phase micro extraction(SPME) and preliminarily identified by GC-MS are nonanoic acid, tetradecanoic acid, pentadecan oic acid, bexadecanoic acid, 2-pentadecanone, 6,10,14-trimethyl-,benzophenone, butylated hydroxytoluene, phenol,4,6-di(1,1-dimethylethyl)-2-methyl,2-buten-1-ol,3-methyl-,1,8-naphthyridin-2-amine,5,7-dimethyl-, 9H-pyrido[3,4-b]indol-7-ol,1-methyl- and 4-hexen-2-yn-1-one,1-phenyl-5-(1-piperi) and so on. The bioassay on selectivity of two kinds of spider mites to G.biloba leaves and apple leaves or clear air was carried out with Y-tube olfactometer. Results showed that the antifeedant rates of two kinds of spider mites against G.biloba leaves are all 100%. The selection rate of T.urticae and T.viennensis to G.biloba leaves in odour source group, G.biloba leaves vs. apple leaves, is 22.2% and 23.5%, respectively. It is 21.3% and 24.8% in G. biloba leaves vs. clear air, respectively. There is highly significant difference between treatments and controls, apple leaves or air. Two kinds of spider mites antifeedant against G. biloba leaves and volatiles from G. biloba leaves are an important factor that affects the selection of spider mites to hosts.展开更多
The influences of Ginkgo biloba leaves on survival and fecundity of Tetranychus urticae and Tetranychus viennensis were elucidated by the bioassay on resistance of G.biloba to spider mites.The mortality curve and ovip...The influences of Ginkgo biloba leaves on survival and fecundity of Tetranychus urticae and Tetranychus viennensis were elucidated by the bioassay on resistance of G.biloba to spider mites.The mortality curve and oviposition curve of spider mites confined in Ginkgo biloba leaf with the method of petri dish were visually analysed,and then the probit analysis simulated the regression relationship between mortality and survival time.Results demonstrated that T.urticae and T.viennensis inoculated on a G.biloba leaf all died respectively in 192 h and 96 h,and there were the normal phase linear regression relations between the mortality and the survival time.The median lethal time(LT_ 50) of T.urticae and T.viennensis on a G.biloba leaf was 102.00 h and 57.32 h,respectively.There was significant difference between the median lethal time(LT_ 50) of treatment and the median mortality time(MT_ 50) of control.The fecundity of T.viennensis inoculated on G.biloba leaves was fully inhibited in four days,and T.urticae never oviposited on ginkgo leaves during the experiment.In comparing the two spider species,ginkgo leaves affected more adversely the survival of T.viennensis,while inhibited more strongly the fecundity of T.urticae.展开更多
文摘The selectivity of Tetranychus urticae and Tetranychus viennensis to Ginkgo biloba leaves was determined by means of method of petri dish, and elucidated the resistance of G.biloba against spider mites, and then the influence of volatiles from G.bioloba leaves on selection behavior of spider mites was explored.Volatiles from G.biloba leaves which were collected with solid phase micro extraction(SPME) and preliminarily identified by GC-MS are nonanoic acid, tetradecanoic acid, pentadecan oic acid, bexadecanoic acid, 2-pentadecanone, 6,10,14-trimethyl-,benzophenone, butylated hydroxytoluene, phenol,4,6-di(1,1-dimethylethyl)-2-methyl,2-buten-1-ol,3-methyl-,1,8-naphthyridin-2-amine,5,7-dimethyl-, 9H-pyrido[3,4-b]indol-7-ol,1-methyl- and 4-hexen-2-yn-1-one,1-phenyl-5-(1-piperi) and so on. The bioassay on selectivity of two kinds of spider mites to G.biloba leaves and apple leaves or clear air was carried out with Y-tube olfactometer. Results showed that the antifeedant rates of two kinds of spider mites against G.biloba leaves are all 100%. The selection rate of T.urticae and T.viennensis to G.biloba leaves in odour source group, G.biloba leaves vs. apple leaves, is 22.2% and 23.5%, respectively. It is 21.3% and 24.8% in G. biloba leaves vs. clear air, respectively. There is highly significant difference between treatments and controls, apple leaves or air. Two kinds of spider mites antifeedant against G. biloba leaves and volatiles from G. biloba leaves are an important factor that affects the selection of spider mites to hosts.
文摘The influences of Ginkgo biloba leaves on survival and fecundity of Tetranychus urticae and Tetranychus viennensis were elucidated by the bioassay on resistance of G.biloba to spider mites.The mortality curve and oviposition curve of spider mites confined in Ginkgo biloba leaf with the method of petri dish were visually analysed,and then the probit analysis simulated the regression relationship between mortality and survival time.Results demonstrated that T.urticae and T.viennensis inoculated on a G.biloba leaf all died respectively in 192 h and 96 h,and there were the normal phase linear regression relations between the mortality and the survival time.The median lethal time(LT_ 50) of T.urticae and T.viennensis on a G.biloba leaf was 102.00 h and 57.32 h,respectively.There was significant difference between the median lethal time(LT_ 50) of treatment and the median mortality time(MT_ 50) of control.The fecundity of T.viennensis inoculated on G.biloba leaves was fully inhibited in four days,and T.urticae never oviposited on ginkgo leaves during the experiment.In comparing the two spider species,ginkgo leaves affected more adversely the survival of T.viennensis,while inhibited more strongly the fecundity of T.urticae.
