The weakness of visible and near-infrared light penetration depth limits the application of photodynamic therapy(PDT)in deep-seated tumors.Based on the high penetrability of X-rays,X-ray-induced PDT(X-PDT)is a promisi...The weakness of visible and near-infrared light penetration depth limits the application of photodynamic therapy(PDT)in deep-seated tumors.Based on the high penetrability of X-rays,X-ray-induced PDT(X-PDT)is a promising new method for treating deep-seated tumors.However,it requires the development of suitable X-ray-induced sensitizers that could employ X-ray energy to produce reactive oxygen species(ROS)efficiently.In this study,a novel X-rayinduced sensitizer(NanoSRF)was developed through a microemulsion method,in which copper iodine cluster compound Cu_(2)I_(2)(tpp)2(2,5-dm-pz)(CIP)and rose bengal(RB)worked as scintillator and photosensitizer,respectively.CIP was synthesized by a simple mechanical grinding method,and subsequently folic acid(FA)-modified albumin was introduced to enable its alliance with RB.NanoSRF exhibited excellent dispersion stability and generated a large amount of ROS under X-ray irradiation.The results of in vitro studies demonstrated its high selectivity for FA receptor-positive cancer cells.Following systemic administration,NanoSRF accumulated in H22 tumors of xenograft-bearing mice,and Xray irradiation(5.46 Gy)induced a significant inhibition rate of 96.7%in tumor growth.This study pioneers the use of copper iodide cluster as a scintillator in X-PDT,presenting new possibilities for designing scintillators with exceptional X-ray absorption and efficient X-PDT capabilities.展开更多
基金supported by the National Natural Science Foundation of China(22178065,22078066 and U1705282).
文摘The weakness of visible and near-infrared light penetration depth limits the application of photodynamic therapy(PDT)in deep-seated tumors.Based on the high penetrability of X-rays,X-ray-induced PDT(X-PDT)is a promising new method for treating deep-seated tumors.However,it requires the development of suitable X-ray-induced sensitizers that could employ X-ray energy to produce reactive oxygen species(ROS)efficiently.In this study,a novel X-rayinduced sensitizer(NanoSRF)was developed through a microemulsion method,in which copper iodine cluster compound Cu_(2)I_(2)(tpp)2(2,5-dm-pz)(CIP)and rose bengal(RB)worked as scintillator and photosensitizer,respectively.CIP was synthesized by a simple mechanical grinding method,and subsequently folic acid(FA)-modified albumin was introduced to enable its alliance with RB.NanoSRF exhibited excellent dispersion stability and generated a large amount of ROS under X-ray irradiation.The results of in vitro studies demonstrated its high selectivity for FA receptor-positive cancer cells.Following systemic administration,NanoSRF accumulated in H22 tumors of xenograft-bearing mice,and Xray irradiation(5.46 Gy)induced a significant inhibition rate of 96.7%in tumor growth.This study pioneers the use of copper iodide cluster as a scintillator in X-PDT,presenting new possibilities for designing scintillators with exceptional X-ray absorption and efficient X-PDT capabilities.
