本文研究了2700 bp DNA片段在金属离子作用下,折叠缩合为复曲面的形成过程。以电子显微镜术为手段,经大量观测抽提出几种典型中间结构,并计算了各典型结构的尺度分布。作者由此推论,DNA缩合结构的形成过程是DNA链折叠与排列的过程;是无...本文研究了2700 bp DNA片段在金属离子作用下,折叠缩合为复曲面的形成过程。以电子显微镜术为手段,经大量观测抽提出几种典型中间结构,并计算了各典型结构的尺度分布。作者由此推论,DNA缩合结构的形成过程是DNA链折叠与排列的过程;是无序到有序的转化过程。研究不仅证实缩合是有规则与有方向的,还进一步证实短DNA片段的缩合是多分子的缩合。展开更多
A detailed study of the formation of toroidal condensation produced from the 2700 base pair fragments of plasmid PUCI3 DNA, induced by metal ions, is introduced. We have extracted several typical intermediate structur...A detailed study of the formation of toroidal condensation produced from the 2700 base pair fragments of plasmid PUCI3 DNA, induced by metal ions, is introduced. We have extracted several typical intermediate structures based on investigation by electron microscopy, and compared their size distributions. The observations suggest that the formation process of DNA condensation is the process of folding and arranging DNA chains and that of disorderorder transition. The result also indicates that the condensed particles are polymeric, but not randomly aggregative, further proving thetoroidal structures are formed in certain regularities.展开更多
文摘本文研究了2700 bp DNA片段在金属离子作用下,折叠缩合为复曲面的形成过程。以电子显微镜术为手段,经大量观测抽提出几种典型中间结构,并计算了各典型结构的尺度分布。作者由此推论,DNA缩合结构的形成过程是DNA链折叠与排列的过程;是无序到有序的转化过程。研究不仅证实缩合是有规则与有方向的,还进一步证实短DNA片段的缩合是多分子的缩合。
基金Project supported by the National Natural Science Foundation of China and the National Education Committee of China
文摘A detailed study of the formation of toroidal condensation produced from the 2700 base pair fragments of plasmid PUCI3 DNA, induced by metal ions, is introduced. We have extracted several typical intermediate structures based on investigation by electron microscopy, and compared their size distributions. The observations suggest that the formation process of DNA condensation is the process of folding and arranging DNA chains and that of disorderorder transition. The result also indicates that the condensed particles are polymeric, but not randomly aggregative, further proving thetoroidal structures are formed in certain regularities.
