A mRNA whole_mount in situ hybridization method is reported here for quick, direct analysis of the spatial and temporal mRNA expression patterns in plant young embryos. A cDNA clone THE 3 (tobacco heart embryo 3) was ...A mRNA whole_mount in situ hybridization method is reported here for quick, direct analysis of the spatial and temporal mRNA expression patterns in plant young embryos. A cDNA clone THE 3 (tobacco heart embryo 3) was isolated by differential screening from tobacco (Nicotiana tabacum L.) heart embryo cDNA library as compared with the globular embryo cDNA library. The distribution of THE 3 mRNA in tobacco heart embryos and globular embryos was investigated by a whole_mount in situ hybridization technique, showing that THE 3 is preferentially expressed in heart embryos.展开更多
Two cDNA libraries were constructed from microdissected 214 rice proembryos (2-3 d after pollination) and 121 just differentiating young embryos (3-5 d after pollination) respectively through RT_PCR technique. The pri...Two cDNA libraries were constructed from microdissected 214 rice proembryos (2-3 d after pollination) and 121 just differentiating young embryos (3-5 d after pollination) respectively through RT_PCR technique. The primary libraries had a total of 3.7×10 6 phages for the proembryos and a total of 2.5×10 6 phages for the just differentiating young embryos, in which 96% of the phages were recombinants. Insert sizes ranging from 400 bp to 3?500 bp were obtained. All of the above mentioned accorded with the general requirements of cDNA library construction.展开更多
文摘A mRNA whole_mount in situ hybridization method is reported here for quick, direct analysis of the spatial and temporal mRNA expression patterns in plant young embryos. A cDNA clone THE 3 (tobacco heart embryo 3) was isolated by differential screening from tobacco (Nicotiana tabacum L.) heart embryo cDNA library as compared with the globular embryo cDNA library. The distribution of THE 3 mRNA in tobacco heart embryos and globular embryos was investigated by a whole_mount in situ hybridization technique, showing that THE 3 is preferentially expressed in heart embryos.
文摘Two cDNA libraries were constructed from microdissected 214 rice proembryos (2-3 d after pollination) and 121 just differentiating young embryos (3-5 d after pollination) respectively through RT_PCR technique. The primary libraries had a total of 3.7×10 6 phages for the proembryos and a total of 2.5×10 6 phages for the just differentiating young embryos, in which 96% of the phages were recombinants. Insert sizes ranging from 400 bp to 3?500 bp were obtained. All of the above mentioned accorded with the general requirements of cDNA library construction.
