In the present study, the chemosensitivity of MGc80-3 human gastric adenocarcinoma cells was determined by means of colony-forming assay and the in vitro activities of 10 anticancer drugs were examined on the basis of...In the present study, the chemosensitivity of MGc80-3 human gastric adenocarcinoma cells was determined by means of colony-forming assay and the in vitro activities of 10 anticancer drugs were examined on the basis of the clinically achievable peak plasma drug concentration. The results showed that MGc80-3 cells were most sensitive to mitomyc'n C, adriamycin and 5-fluorouracil, being consistent with the response noted in clinical gastric cancer. This cell line may retain its original drug sensitivity and may be useful in screening for new compounds with activity against this disease.展开更多
Colony forming assay, i.e. clonogenic assay is known to be the most reliable technique for testing reproductive capacity of malignant cells and is widely used in experimental chemotherapy and other biological studies....Colony forming assay, i.e. clonogenic assay is known to be the most reliable technique for testing reproductive capacity of malignant cells and is widely used in experimental chemotherapy and other biological studies. Up to now, approximately 50 human tumor cell lines and their clones have been established in China, but the technique for colony formation of these cells does not as yet appear to have been studied systematically. Biop-展开更多
文摘In the present study, the chemosensitivity of MGc80-3 human gastric adenocarcinoma cells was determined by means of colony-forming assay and the in vitro activities of 10 anticancer drugs were examined on the basis of the clinically achievable peak plasma drug concentration. The results showed that MGc80-3 cells were most sensitive to mitomyc'n C, adriamycin and 5-fluorouracil, being consistent with the response noted in clinical gastric cancer. This cell line may retain its original drug sensitivity and may be useful in screening for new compounds with activity against this disease.
文摘Colony forming assay, i.e. clonogenic assay is known to be the most reliable technique for testing reproductive capacity of malignant cells and is widely used in experimental chemotherapy and other biological studies. Up to now, approximately 50 human tumor cell lines and their clones have been established in China, but the technique for colony formation of these cells does not as yet appear to have been studied systematically. Biop-