人肝癌细胞系的药物敏感性研究

采用集落形成试验,测定五种人肝癌细胞系的药物敏感性。结果表明,这些细胞系对药物明显抗拒,对ADM、5-Fu、DDP和MEL的敏感性依次降低。各系细胞对PYM均较敏感。与ADM显然不同,PYM对慢增殖细胞具有更强的杀伤作用。该组细胞系似可用于抗肝癌药物的筛选。

平阳霉素的体外抗癌谱

采用集落形成试验法,对平阳霉素(pingyangmycin,PYM)的体外抗癌谱进行研究,结果10种人癌细胞系对PYM的敏感性存在差异,ID_(50)为0.03～0.82μg/ml。食管癌Eca109和CaEs17细胞、鼻咽癌CNE细胞及肝癌BEL-7402细胞对PYM敏感,ID_(50)<0.2μg/ml,与PYM的临床疗效一致;2种起源于宫颈癌的细胞系对PYM明显抗拒,ID_(50)>0.7μg/ml,与临床疗效不一致;2种胃腺癌细胞系(MGc80-3和BGC-823)对PYM的敏感性较低,ID_(50)为0.21和0.47μg/ml;肺鳞癌LTEP-78为PYM敏感细胞,ID_(50)仅为肺腺癌SPC-A-1细胞的1/7,但其临床意义尚不清楚。

CHEMOSENSITIVITY OF MGc80-3 HUMAN GASTRIC ADENOCARCINOMA CELLS AND ITS CLINICAL SIGNIFICANCE

In the present study, the chemosensitivity of MGc80-3 human gastric adenocarcinoma cells was determined by means of colony-forming assay and the in vitro activities of 10 anticancer drugs were examined on the basis of the clinically achievable peak plasma drug concentration. The results showed that MGc80-3 cells were most sensitive to mitomyc'n C, adriamycin and 5-fluorouracil, being consistent with the response noted in clinical gastric cancer. This cell line may retain its original drug sensitivity and may be useful in screening for new compounds with activity against this disease.

COMPARISON OF METHODS FOR COLONY FORMATION OF ESTABLISHED HUMAN CANCER CELL LINES

Colony forming assay, i.e. clonogenic assay is known to be the most reliable technique for testing reproductive capacity of malignant cells and is widely used in experimental chemotherapy and other biological studies. Up to now, approximately 50 human tumor cell lines and their clones have been established in China, but the technique for colony formation of these cells does not as yet appear to have been studied systematically. Biop-