Blood samples were collected from chicken embryos at stage 11~15, and labeled with fluorescent dye PKH26. Primordial germ cells (PGCs) were then isolated from blood samples by nycodenz density gradient centrifugation...Blood samples were collected from chicken embryos at stage 11~15, and labeled with fluorescent dye PKH26. Primordial germ cells (PGCs) were then isolated from blood samples by nycodenz density gradient centrifugation. After PGCs were labeled and isolated, about 200 PGCs in one microliter were injected into the subgerminal cavity of quail blastoderm at stageⅩ. After 48 hours incubation, chicken PGCs were identified by fluorescent microscopy. Red fluorescence emitted from PKH26 labeled chicken PGCs was observed in the head, the heart and the developing gonadal anlage of quail embryos. The result suggests that chicken PGCs still keep migration ability after 56 hours.展开更多
文摘Blood samples were collected from chicken embryos at stage 11~15, and labeled with fluorescent dye PKH26. Primordial germ cells (PGCs) were then isolated from blood samples by nycodenz density gradient centrifugation. After PGCs were labeled and isolated, about 200 PGCs in one microliter were injected into the subgerminal cavity of quail blastoderm at stageⅩ. After 48 hours incubation, chicken PGCs were identified by fluorescent microscopy. Red fluorescence emitted from PKH26 labeled chicken PGCs was observed in the head, the heart and the developing gonadal anlage of quail embryos. The result suggests that chicken PGCs still keep migration ability after 56 hours.