The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function mutant.Here,we provide more evidence to support this view that S/SGR1 is involve...The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function mutant.Here,we provide more evidence to support this view that S/SGR1 is involved in color change in ripening tomato fruits.Knocking out SISGR1 expression using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing strategy showed obviously a muddy brown color with significantly higher chlorophyll and carotenoid content compared with wild-type(WT)fruits.To further verify the role of SiSGR1 in fruit color change,we performed transcriptome deep sequencing(RNA-seq)analysis,where a total of 354 differentially expressed genes(124/230 downregulated/upregulated)were identified between WT and slsgr1.Additionally,the expression of numerous genes associated with photosynthesis and chloroplast function changed significantly when S/SGR1 was knocked out.Taken together,these results indicate that S/SGR1 is involved in color change in ripening fruit via chlorophyll degradation and carotenoid biosynthesis.展开更多
基金supported by the National Natural Science Foundation of China (No.31972472 and No.32061143022).
文摘The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function mutant.Here,we provide more evidence to support this view that S/SGR1 is involved in color change in ripening tomato fruits.Knocking out SISGR1 expression using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing strategy showed obviously a muddy brown color with significantly higher chlorophyll and carotenoid content compared with wild-type(WT)fruits.To further verify the role of SiSGR1 in fruit color change,we performed transcriptome deep sequencing(RNA-seq)analysis,where a total of 354 differentially expressed genes(124/230 downregulated/upregulated)were identified between WT and slsgr1.Additionally,the expression of numerous genes associated with photosynthesis and chloroplast function changed significantly when S/SGR1 was knocked out.Taken together,these results indicate that S/SGR1 is involved in color change in ripening fruit via chlorophyll degradation and carotenoid biosynthesis.
