A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface...A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface morphology. Electrodeposited Pd nanoparticles exhibited efficiently electrocatalytic oxidation for hydrogen peroxide (H_2O_2) with relatively high sensitivity and stability,which was studied by CV technique and Raman spectroscopy,respectively. The GC/Pd/GOD/Nafion system allowed a low working potential of +0.3 V (vs. SCE). Its signal current was linearly related to the glucose concentration in the range of 1.0×10 -6 —1.2×10 -4 mol·L -1 with a detection limit of 5.0×10 -7 mol·L -1 . The sensor required no special pretreatment to suppress interference from urate and L-ascorbate. It was successfully used in detection of glucose level in human urine with high stability,sensitivity and anti-poisoning ability .展开更多
Amperometric sensor based on neutral red-doped silica （NRSiO2） nanoparticles （NPs） was fabricated and coupled with a microdialysis sampling system for the detection of glutamate （Glu） in the rat striatum. The NR...Amperometric sensor based on neutral red-doped silica （NRSiO2） nanoparticles （NPs） was fabricated and coupled with a microdialysis sampling system for the detection of glutamate （Glu） in the rat striatum. The NRSiO2 NPs [about （45 ± 3） nm] were prepared with water-in-oil （W/O） microemulsion method, and characterized by transmission electron microscope （TEM） technique. The neutral red （NR） doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0×10^-7 to 1.5×10^-4 mol/L, with a detection limit of 2.0×10^-7 mol/L （S/N=3）.展开更多
基金ProjectsupportedbytheNaturalScienceFoundationofChina (No .2 0 175 0 0 6)andtheSpecialFundationforNanoTechnologyofShanghai (No .0 2 14nm0 78andNo .0 114nm 0 72 )
文摘A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface morphology. Electrodeposited Pd nanoparticles exhibited efficiently electrocatalytic oxidation for hydrogen peroxide (H_2O_2) with relatively high sensitivity and stability,which was studied by CV technique and Raman spectroscopy,respectively. The GC/Pd/GOD/Nafion system allowed a low working potential of +0.3 V (vs. SCE). Its signal current was linearly related to the glucose concentration in the range of 1.0×10 -6 —1.2×10 -4 mol·L -1 with a detection limit of 5.0×10 -7 mol·L -1 . The sensor required no special pretreatment to suppress interference from urate and L-ascorbate. It was successfully used in detection of glucose level in human urine with high stability,sensitivity and anti-poisoning ability .
基金Project supported by the National Natural Science Foundation of China （Nos. 20175006, 20305007） and Doctoral Foundation of China Ministry of Education （No. 20030269014）.
文摘Amperometric sensor based on neutral red-doped silica （NRSiO2） nanoparticles （NPs） was fabricated and coupled with a microdialysis sampling system for the detection of glutamate （Glu） in the rat striatum. The NRSiO2 NPs [about （45 ± 3） nm] were prepared with water-in-oil （W/O） microemulsion method, and characterized by transmission electron microscope （TEM） technique. The neutral red （NR） doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0×10^-7 to 1.5×10^-4 mol/L, with a detection limit of 2.0×10^-7 mol/L （S/N=3）.
