为阐明牛初乳、牛常乳乳清蛋白的差异,利用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)蛋白质组学技术对二者进行蛋白质组差异分析,在得到的599种具有定量信息的乳清蛋白中,鉴定出60种差异...为阐明牛初乳、牛常乳乳清蛋白的差异,利用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)蛋白质组学技术对二者进行蛋白质组差异分析,在得到的599种具有定量信息的乳清蛋白中,鉴定出60种差异蛋白。将牛初乳与牛常乳乳清丰度差异蛋白进行生物信息学分析发现,差异蛋白主要参与的生物过程为转运、定位、单一生物作用等;主要参与的分子功能为顶端质膜、细胞外区域、细胞外区域部分等;主要参与的细胞组成为蛋白结合和阴离子结合。丰度差异蛋白中有9种是与信号传导相关,有6种糖基化乳清蛋白。此外,利用蛋白质网络互作分析发现,差异蛋白中存在具有高连接度的关键乳清蛋白因子。本研究采用iTRAO技术对牛初乳与牛常乳乳清差异蛋白进行鉴定及生物信息学分析,为今后改善牛初、常乳品质,开发婴幼儿乳粉以及功能性乳制品提供了一定参考。展开更多
Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed...Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed the senescence of pak choi stored at 20°C.A total of 1121 differentially expressed genes(DEGs)were identified in an MT-treated group in comparison to untreated pak choi.Analysis of DEGs revealed that MT-induced delay in senescence involved reduction in the expression of chlorophyll metabolism genes and regulation of the expression of hormone-related genes and leaf senescence-related transcription factors.MT decreased the activity of chlorophyll metabolism-related enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase,decreased the concentrations of abscisic acid and jasmonic acid,and increased auxin concentration.MT delayed the impact of senescence-related transcription factors BcNAC41 and BcNAC87 on tobacco leaf senescence.This study provides an insight into the molecular mechanisms of MT-induced delay of postharvest senescence in pak choi and confirms its potential as a preservation technique.展开更多
文摘为阐明牛初乳、牛常乳乳清蛋白的差异,利用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)蛋白质组学技术对二者进行蛋白质组差异分析,在得到的599种具有定量信息的乳清蛋白中,鉴定出60种差异蛋白。将牛初乳与牛常乳乳清丰度差异蛋白进行生物信息学分析发现,差异蛋白主要参与的生物过程为转运、定位、单一生物作用等;主要参与的分子功能为顶端质膜、细胞外区域、细胞外区域部分等;主要参与的细胞组成为蛋白结合和阴离子结合。丰度差异蛋白中有9种是与信号传导相关,有6种糖基化乳清蛋白。此外,利用蛋白质网络互作分析发现,差异蛋白中存在具有高连接度的关键乳清蛋白因子。本研究采用iTRAO技术对牛初乳与牛常乳乳清差异蛋白进行鉴定及生物信息学分析,为今后改善牛初、常乳品质,开发婴幼儿乳粉以及功能性乳制品提供了一定参考。
基金the National Natural Science Foundation of China(No.32001451)the Key and General Programs of Modern Agriculture in Jiangsu Province(No.BE2022368),China.
文摘Melatonin(MT)is a low molecular weight compound with a wide variety of biological functions in plants.It is known to delay senescence in various fruits and vegetables.This study demonstrates that MT(100μmol/L)delayed the senescence of pak choi stored at 20°C.A total of 1121 differentially expressed genes(DEGs)were identified in an MT-treated group in comparison to untreated pak choi.Analysis of DEGs revealed that MT-induced delay in senescence involved reduction in the expression of chlorophyll metabolism genes and regulation of the expression of hormone-related genes and leaf senescence-related transcription factors.MT decreased the activity of chlorophyll metabolism-related enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase,decreased the concentrations of abscisic acid and jasmonic acid,and increased auxin concentration.MT delayed the impact of senescence-related transcription factors BcNAC41 and BcNAC87 on tobacco leaf senescence.This study provides an insight into the molecular mechanisms of MT-induced delay of postharvest senescence in pak choi and confirms its potential as a preservation technique.
