水稻SDG723蛋白C末端原核表达及多克隆抗体制备

水稻SDG723蛋白含有植物组蛋白甲基转移酶保守的SET结构域。选取其抗原决定簇较密集的C末端进行原核表达,通过构建原核表达载体pET28a-723C,转化E.coli BL21(DE3)感受态细胞,IPTG诱导表达融合蛋白后进行纯化。以纯化的融合蛋白为抗原免疫新西兰白兔,制备多克隆抗体。Western-blot分析表明,制备的多克隆抗体能有效地检测抗原的表达,为进一步深入研究SDG723蛋白的功能奠定了基础。

Prokaryotic Expression and Polyclonal Antibody Preparation of SDG711 C-terminal from Rice

[Objective] This study aimed to conduct prokaryotic expression of rice SDG711 C-terminal and to prepare its polyclonal antibody. [Method] C-terminal of rice SDG711 containing relatively intensive antigen determinants was selected for prokaryotic expression, prokaryotic expression vector pET28a-711C was constructed and the recombinant plasmid was transformed into Escherichia coli BL21 （DE3） competent cells. The recombinant fusion protein was induced by IPTG and purified to immunize a New Zealand white rabbit as the antigen, and polyclonal antibody was obtained and confirmed by Western-blot analysis. [Result] The polyclonal anti- body was successfully prepared and could efficiently detect the expressed antigen. [Conclusion] This study laid the foundation for further investigating the functions of SDG711 protein in rice.

水稻SDG711蛋白C末端原核表达及多克隆抗体制备

[目的]对水稻SDG711蛋白C末端进行原核表达,并制备其多克隆抗体。[方法]选取水稻SDG711蛋白抗原决定簇较密集的C末端进行原核表达,通过构建原核表达载体pET28a-711C,转化E.coli BL21(DE3)感受态细胞,IPTG诱导表达融合蛋白后进行纯化,再以纯化的融合蛋白为抗原免疫新西兰白兔,制备多克隆抗体,并对其进行Western-blot分析。[结果]试验制备的多克隆抗体能有效地检测抗原的表达。[结论]该研究为进一步深入研究SDG711蛋白的功能奠定了基础。