本研究基于免培养和高通量测序技术,对金钗石斛种子内生菌16 S rDNA V 4区和ITS 1区扩增序列进行分析,揭示内生菌的组成和丰度。结果表明:扩增的内生细菌16 S rDNA序列归为13 OTUs,隶属于变形菌门(Proteobacteria)和蓝细菌门(Cyanobacte...本研究基于免培养和高通量测序技术,对金钗石斛种子内生菌16 S rDNA V 4区和ITS 1区扩增序列进行分析,揭示内生菌的组成和丰度。结果表明:扩增的内生细菌16 S rDNA序列归为13 OTUs,隶属于变形菌门(Proteobacteria)和蓝细菌门(Cyanobacteria);其中变形菌门为优势门,泛菌属(Pantoea)为优势属;香农指数、辛普森指数和Chao 1指数分别为1.82、0.69和11.00。内生真菌序列归为14个OTUs,隶属于子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和接合菌门(Zygomycota);其中子囊菌门为优势门,德巴利酵母属(Debaryomyces)为优势属;香农指数、辛普森指数和Chao1指数分别为0.57、0.79和14.13。金钗石斛种子的内生细菌与内生真菌具有一定的多样性。此研究为进一步促进金钗石斛种子萌发及幼苗栽培具有一定的指导意义。展开更多
[Objective] The biological safety of white insect tea in Xifeng County of Guizhou Province was evaluated in order to provide a scientific basis for the development of white insect tea. [Method] Acute toxicity test in ...[Objective] The biological safety of white insect tea in Xifeng County of Guizhou Province was evaluated in order to provide a scientific basis for the development of white insect tea. [Method] Acute toxicity test in mice, Ames microbiological test and mouse bone marrow polychromatic erythrocyte micronucleus test were done with wild white inset tea produced in Xifeng County. At the same time, the contents of six kinds of limit pollutants were determined, including Pb,Cr,Cd,Hg,As,and F-. [Result] The results showed that white insect tea had LD505.0 g/kg（BW）in acute toxicity test, negative response in Ames test and microkernel test. In addition, the contents of Pb,Cr,Cd,Hg,As,and F- in the white inset tea were 2.20,1.06,0.46,0.09,0.71,21.50 mg/kg, much less than the national standard of tea （GB2762-2005,NY 659-2003）. [Conclusion] The white insect tea produced in Xifeng County of Guizhou Province is non-toxic, and good for human as beverage.展开更多
以引种辣椒Jalapeno Early Hot、Portugal Hot、Sweet Banana为材料,利用自动灌溉系统控制基质不同含水量,包括5%、10%、、15%、20%、25%(CK),研究3个辣椒品种对干旱胁迫的响应。结果表明:随着基质含水量的减少,3个品种叶面积、叶绿素S...以引种辣椒Jalapeno Early Hot、Portugal Hot、Sweet Banana为材料,利用自动灌溉系统控制基质不同含水量,包括5%、10%、、15%、20%、25%(CK),研究3个辣椒品种对干旱胁迫的响应。结果表明:随着基质含水量的减少,3个品种叶面积、叶绿素SPAD值、相对含水量和水势均呈下降趋势,而根干重、根冠比和脯氨酸含量则逐渐升高;基质相对含水量为5%时,Jalapeno Early Hot的叶面积、叶绿素含量、相对含水量、水势分别比对照下降9.79%、10.76%、8.03%和79.45%,根干重、根冠比和脯氨酸增加19.43%、26.82%和201.85%,Sweet Banana的叶面积、叶绿素含量、相对含水量、水势比对照分别下降18.05%、13.51%、17.70%和149.23%,根干重、根冠比和脯氨酸增加28.79%、33.17%和115.19%。说明可以通过叶片特征的调整使辣椒适应干旱胁迫,其中Jalapeno Early Hot最强,Portugal Hot居中,Sweet Banana适应能力最弱。展开更多
文摘本研究基于免培养和高通量测序技术,对金钗石斛种子内生菌16 S rDNA V 4区和ITS 1区扩增序列进行分析,揭示内生菌的组成和丰度。结果表明:扩增的内生细菌16 S rDNA序列归为13 OTUs,隶属于变形菌门(Proteobacteria)和蓝细菌门(Cyanobacteria);其中变形菌门为优势门,泛菌属(Pantoea)为优势属;香农指数、辛普森指数和Chao 1指数分别为1.82、0.69和11.00。内生真菌序列归为14个OTUs,隶属于子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和接合菌门(Zygomycota);其中子囊菌门为优势门,德巴利酵母属(Debaryomyces)为优势属;香农指数、辛普森指数和Chao1指数分别为0.57、0.79和14.13。金钗石斛种子的内生细菌与内生真菌具有一定的多样性。此研究为进一步促进金钗石斛种子萌发及幼苗栽培具有一定的指导意义。
基金Supported by Science and Technology Foundation of Guizhou Province(LKS[2009]No.24)Technological Innovation Team-building Projects of Guizhou Province([2009]4007)~~
文摘[Objective] The biological safety of white insect tea in Xifeng County of Guizhou Province was evaluated in order to provide a scientific basis for the development of white insect tea. [Method] Acute toxicity test in mice, Ames microbiological test and mouse bone marrow polychromatic erythrocyte micronucleus test were done with wild white inset tea produced in Xifeng County. At the same time, the contents of six kinds of limit pollutants were determined, including Pb,Cr,Cd,Hg,As,and F-. [Result] The results showed that white insect tea had LD505.0 g/kg（BW）in acute toxicity test, negative response in Ames test and microkernel test. In addition, the contents of Pb,Cr,Cd,Hg,As,and F- in the white inset tea were 2.20,1.06,0.46,0.09,0.71,21.50 mg/kg, much less than the national standard of tea （GB2762-2005,NY 659-2003）. [Conclusion] The white insect tea produced in Xifeng County of Guizhou Province is non-toxic, and good for human as beverage.
文摘以引种辣椒Jalapeno Early Hot、Portugal Hot、Sweet Banana为材料,利用自动灌溉系统控制基质不同含水量,包括5%、10%、、15%、20%、25%(CK),研究3个辣椒品种对干旱胁迫的响应。结果表明:随着基质含水量的减少,3个品种叶面积、叶绿素SPAD值、相对含水量和水势均呈下降趋势,而根干重、根冠比和脯氨酸含量则逐渐升高;基质相对含水量为5%时,Jalapeno Early Hot的叶面积、叶绿素含量、相对含水量、水势分别比对照下降9.79%、10.76%、8.03%和79.45%,根干重、根冠比和脯氨酸增加19.43%、26.82%和201.85%,Sweet Banana的叶面积、叶绿素含量、相对含水量、水势比对照分别下降18.05%、13.51%、17.70%和149.23%,根干重、根冠比和脯氨酸增加28.79%、33.17%和115.19%。说明可以通过叶片特征的调整使辣椒适应干旱胁迫,其中Jalapeno Early Hot最强,Portugal Hot居中,Sweet Banana适应能力最弱。
