Poly( N isopropylacrylamide methylacrylic acid)[P(NIP co MAA)], a linear water soluble pH precipitating synthetic polymer, was used as a novel separating carrier for the reactants in immunoassay. The P(NIP co MAA) pre...Poly( N isopropylacrylamide methylacrylic acid)[P(NIP co MAA)], a linear water soluble pH precipitating synthetic polymer, was used as a novel separating carrier for the reactants in immunoassay. The P(NIP co MAA) precipitates out of water below a critical pH 5.6 at 37 ℃, and redissolved when the solution pH is above 6.1. This characteristic of the P(NIP co MAA) made it possible to carry out the immunochemical steps of the assay in true solution and then to quickly separate the resulting procucts from the reaction mixtures. The above approach was applied to an assay for the sandwich immunoassay of hepatitis B surface antigen(HBsAg). Sensitivity of this method was close to that of traditional ELISA using same reactants. However, the assay was much faster(assay time decreased from 100—120 min to 45 min). This method has been applied to the determination of the HBsAg levels in human blood serum with satisfactory results. This general technique may also be used for a wide variety of separation processes in addition to immunoassay, in which a specific component is to be isolated for analysis, recovery, or disposal. [WT5HZ]展开更多
To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for...To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively.Based on this method,the HPLC fingerprints of Radix gentianae were established.Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic acid.There is no report of the compounds in chloroform phase.The content of gentiopicroside and loganic acid in samples of different groups were determined,separately.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within 0.62-0.99.This method can be used for determination of potent component in Radix gentianae and its quality control.Radix gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.展开更多
Tetra substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red region fluorescent substrate for the determination of hydrogen peroxide catalyzed by peroxidase or...Tetra substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red region fluorescent substrate for the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear range of 0.0-3.0×10 -7 mol/L H 2O 2 with a detection limit of 3.7×10 -9 mol/L. The feasibility of TAAlPc as a new promising red region substrate in practical application has been proven in the determination of H 2O 2 in rainwater.The proposed method can largely minimize the interference that results from background fluorescence or scattering light and has a high analytical sensitivity.展开更多
Recent advances in the nanomaterials, such as luminescent quantum dots, latex fluorescent nanospheres and dye-doped silica nanoparticles, have opened a promising field toward the development of luminescent biolabel. I...Recent advances in the nanomaterials, such as luminescent quantum dots, latex fluorescent nanospheres and dye-doped silica nanoparticles, have opened a promising field toward the development of luminescent biolabel. In this paper, we develop a kind of novel nanometer-sized fluorescent hybrid silica(NFHS) particles used as a sensitive and photostable fluorescent probe in biological staining and diagnostics. The NFHS particles are prepared by controlled hydrolysis of the fluorophore silica precursor using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. In comparison with single organic fluorophores without incorporation, these nanoparticle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission(blinking). The NFHS particles have also shown unique advantages over the existing common organic fluorophores, quantum dots, and latex-based fluorescent particles for biomolecule recognition in the following four major points: easy preparation, good photostability, high sensitivity, and low toxicity. The approach proposed in this article for making NFHS nanoparticles is a general one, and it is not restricted to a particular type of fluorophore molecule as selected in this study.展开更多
文摘Poly( N isopropylacrylamide methylacrylic acid)[P(NIP co MAA)], a linear water soluble pH precipitating synthetic polymer, was used as a novel separating carrier for the reactants in immunoassay. The P(NIP co MAA) precipitates out of water below a critical pH 5.6 at 37 ℃, and redissolved when the solution pH is above 6.1. This characteristic of the P(NIP co MAA) made it possible to carry out the immunochemical steps of the assay in true solution and then to quickly separate the resulting procucts from the reaction mixtures. The above approach was applied to an assay for the sandwich immunoassay of hepatitis B surface antigen(HBsAg). Sensitivity of this method was close to that of traditional ELISA using same reactants. However, the assay was much faster(assay time decreased from 100—120 min to 45 min). This method has been applied to the determination of the HBsAg levels in human blood serum with satisfactory results. This general technique may also be used for a wide variety of separation processes in addition to immunoassay, in which a specific component is to be isolated for analysis, recovery, or disposal. [WT5HZ]
文摘To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively.Based on this method,the HPLC fingerprints of Radix gentianae were established.Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic acid.There is no report of the compounds in chloroform phase.The content of gentiopicroside and loganic acid in samples of different groups were determined,separately.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within 0.62-0.99.This method can be used for determination of potent component in Radix gentianae and its quality control.Radix gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.
文摘Tetra substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red region fluorescent substrate for the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear range of 0.0-3.0×10 -7 mol/L H 2O 2 with a detection limit of 3.7×10 -9 mol/L. The feasibility of TAAlPc as a new promising red region substrate in practical application has been proven in the determination of H 2O 2 in rainwater.The proposed method can largely minimize the interference that results from background fluorescence or scattering light and has a high analytical sensitivity.
文摘Recent advances in the nanomaterials, such as luminescent quantum dots, latex fluorescent nanospheres and dye-doped silica nanoparticles, have opened a promising field toward the development of luminescent biolabel. In this paper, we develop a kind of novel nanometer-sized fluorescent hybrid silica(NFHS) particles used as a sensitive and photostable fluorescent probe in biological staining and diagnostics. The NFHS particles are prepared by controlled hydrolysis of the fluorophore silica precursor using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. In comparison with single organic fluorophores without incorporation, these nanoparticle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission(blinking). The NFHS particles have also shown unique advantages over the existing common organic fluorophores, quantum dots, and latex-based fluorescent particles for biomolecule recognition in the following four major points: easy preparation, good photostability, high sensitivity, and low toxicity. The approach proposed in this article for making NFHS nanoparticles is a general one, and it is not restricted to a particular type of fluorophore molecule as selected in this study.
