Objective:To explore the effects of electroacupuncture pretreatment on mitochondrial energy metabolism in the rats with myocardial ischemia reperfusion injury(MIRI).Methods:A total of 60 SPF Wistar rats were randomly ...Objective:To explore the effects of electroacupuncture pretreatment on mitochondrial energy metabolism in the rats with myocardial ischemia reperfusion injury(MIRI).Methods:A total of 60 SPF Wistar rats were randomly divided into a sham-operation group(sham group),a myocardial ischemia reperfusion injury group(MIRI group)and an electroacupuncture pretreatment group(EA group),20 rats in each one.The rats in the sham group and the MIRI group were binded for 7 days,once a day,20 min each time.On the 8th day,the sample was collected after the heart exposed for 50 min in thoractomy in the sham group and the sample was collected after ischemia for 20 min and reperfusion for 30 min in thoractomy in the MIRI group.In the EA group,the pretreatment intervention with electroacupuncture was applied at"Neiguan(内关PC6)","Guanyuan(关元CV4)"and"Zusanli(足三里ST36)"in the rats for 7 days,once a day,20 min each time.On the 8th day,after ischemia for 20 min and reperfusion for 30 min in thoractomy,the sample was collected in the EA group.The changes in STⅡsegment of electroacardiogram(ECG)were observed and measured.Using enzymelinked immunosorbent assay(ELISA),the concentrations of cardiac troponin T(cTnT)and cardiac troponin I(cTnl)were detected.Using nitro blue tetrazolium chloride monohydrate(NBT)staining,the myocardial infarction weight percentage was measured.Using ELISA,the concentrations of mitochondrial adenosine monophosphate(AMP),adenosine diphosphate(ADP)and adenosine triphosphate(ATP)were detected.Results:(1)STⅡchanges:in 20 min of ligation,compared with the sham group,the STⅡsegment of electrocardiograph(ECG)was elevated significantly in the MIRI group and EA group(both P<0.01),but the elevation range in the EA group was lower than that of the MIRI group(P<0.01).After reperfusion for30 min,the STⅡsegment was fallen by over 50%in the MIRI group and the EA group.Simultaneously,the STⅡsegment in the EA group was lower than that of the MIRI group(P<0.01).(2)Regarding myocardial infarction weight percentage,compared with the sham group,the infarction weight was larger in the MIRI group and the EA group(both P<0.05)and the infarction weight in the EA group was lower than that of the MIRI group(P<0.05).(3)Regarding the levels of serum cTnt and cTnI,compared with the sham group,the levels of serum cTnT and cTnI were higher in the MIRI group and the EA group(all P<0.01)and the levels of cTnT and cTnI in the EA group were lower than that of the MIRI group(both P<0.01).(4)Regarding the concentrations of AMP,ADP and ATP,compared with the sham group,ATP concentration was lower in the MIRI group and the EA group(both P<0.01)and the concentrations of AMP and ADP were higher(P<0.05,P<0.01).Compared with the MIRI group,ATP concentration was higher in the EA group(P<0.05)and the concentrations of AMP and ADP were lower(both P<0.01).Conclusions:Electroacupuncture pretreatment reduces the elevation of ECG STⅡsegment,decreases the concentrations of myocardial injury markers,cTnT and cTnI and regulates the transfer among AMP,ATP and ADP.The protective effect of electroacupuncture pretreatment may result from the regulation of mitochondrial energy metabolism.展开更多
Objective:To explore core acupoints and acupoint selection principles in acupuncture and moxibustion for obesity,from syndrome differentiation prescriptions of the acupuncture-moxibustion therapy in 808 obesity prescr...Objective:To explore core acupoints and acupoint selection principles in acupuncture and moxibustion for obesity,from syndrome differentiation prescriptions of the acupuncture-moxibustion therapy in 808 obesity prescriptions,by using node centrality and cluster analysis methods in complex network.Methods:Firstly,an acupoint network model is established,and acupoint nodes are assessed and calculated in multiple aspects by introducing the node centrality analysis idea of complex network,to excavate core acupoint nodes.Secondly,a cluster analysis is carried out on acupoint network by the cluster algorithm Q-PSO for complex network,to investigate the acupoint combination principles.Results:Zusanli(足三里ST36),Tianshu(天枢ST25),Fenglong(丰隆ST40),Zhongwan(中脘CV12)and Qihai(气海CV6),etc.,were included into the core acupoint Sanyinjiao(三阴交SP6)community.Zhigou(支沟TE6),Neiting(内庭ST44),Shangjuxu(上巨虚ST37),and Pishu(脾俞BL20)etc.,were included into the core acupoint Yinlingquan(阴陵泉SP9)community.Baihuanshu(白环俞BL30)and Zhiyang(至阳GV9)were included into the core acupoint Dachangshu(大肠俞BL25)community.Biguan(髀关ST31)was a single core community.Among all the acupoint nodes,SP6,ST25,SP9,ST36,CV6,Quchi(曲池L111),and Guanyuan(关元CV4)were of high degree centrality and eigenvector centrality,directly reflecting their importance in acupoint selection prescriptions.Conclusion:The Q-PSO algorithm is characterized with high precision and high efficiency,etc.The core acupoints and their combination principles explored by this algorithm are in accordance with clinical experiences.展开更多
基金Supported by National Natural Science Foundation of China Youth Program:81704142Hubei Science and Technology Plan Project:2018CFC890+7 种基金The Twelfth Batch of Self-Selected Research Projects(“Collaborative Innovation”Special Fund):YZ-1845Qihuang Project of National Administration of Traditional Chinese Medicine(National Administration of Traditional Chinese Medicine Yard[2018]:284)Hubei Chinese Medicine Teacher Project(Bulletin of Hubei Health and Family Planning Commission[2018]:15)Hubei Hospital of Traditional Chinese Medicine,the First Tanhualin Famous Doctor,Student Training ProjectHubei Administration of Traditional Chinese Medicine[2018]No.72Wuhan Young and Middle-aged Medical Backbone Talents(Sixth Batch)No.116Family Planning Tong[2018]Young Elite Scientist Sponsors Hip Program by CAST:No.2017QNRC001。
文摘Objective:To explore the effects of electroacupuncture pretreatment on mitochondrial energy metabolism in the rats with myocardial ischemia reperfusion injury(MIRI).Methods:A total of 60 SPF Wistar rats were randomly divided into a sham-operation group(sham group),a myocardial ischemia reperfusion injury group(MIRI group)and an electroacupuncture pretreatment group(EA group),20 rats in each one.The rats in the sham group and the MIRI group were binded for 7 days,once a day,20 min each time.On the 8th day,the sample was collected after the heart exposed for 50 min in thoractomy in the sham group and the sample was collected after ischemia for 20 min and reperfusion for 30 min in thoractomy in the MIRI group.In the EA group,the pretreatment intervention with electroacupuncture was applied at"Neiguan(内关PC6)","Guanyuan(关元CV4)"and"Zusanli(足三里ST36)"in the rats for 7 days,once a day,20 min each time.On the 8th day,after ischemia for 20 min and reperfusion for 30 min in thoractomy,the sample was collected in the EA group.The changes in STⅡsegment of electroacardiogram(ECG)were observed and measured.Using enzymelinked immunosorbent assay(ELISA),the concentrations of cardiac troponin T(cTnT)and cardiac troponin I(cTnl)were detected.Using nitro blue tetrazolium chloride monohydrate(NBT)staining,the myocardial infarction weight percentage was measured.Using ELISA,the concentrations of mitochondrial adenosine monophosphate(AMP),adenosine diphosphate(ADP)and adenosine triphosphate(ATP)were detected.Results:(1)STⅡchanges:in 20 min of ligation,compared with the sham group,the STⅡsegment of electrocardiograph(ECG)was elevated significantly in the MIRI group and EA group(both P<0.01),but the elevation range in the EA group was lower than that of the MIRI group(P<0.01).After reperfusion for30 min,the STⅡsegment was fallen by over 50%in the MIRI group and the EA group.Simultaneously,the STⅡsegment in the EA group was lower than that of the MIRI group(P<0.01).(2)Regarding myocardial infarction weight percentage,compared with the sham group,the infarction weight was larger in the MIRI group and the EA group(both P<0.05)and the infarction weight in the EA group was lower than that of the MIRI group(P<0.05).(3)Regarding the levels of serum cTnt and cTnI,compared with the sham group,the levels of serum cTnT and cTnI were higher in the MIRI group and the EA group(all P<0.01)and the levels of cTnT and cTnI in the EA group were lower than that of the MIRI group(both P<0.01).(4)Regarding the concentrations of AMP,ADP and ATP,compared with the sham group,ATP concentration was lower in the MIRI group and the EA group(both P<0.01)and the concentrations of AMP and ADP were higher(P<0.05,P<0.01).Compared with the MIRI group,ATP concentration was higher in the EA group(P<0.05)and the concentrations of AMP and ADP were lower(both P<0.01).Conclusions:Electroacupuncture pretreatment reduces the elevation of ECG STⅡsegment,decreases the concentrations of myocardial injury markers,cTnT and cTnI and regulates the transfer among AMP,ATP and ADP.The protective effect of electroacupuncture pretreatment may result from the regulation of mitochondrial energy metabolism.
基金Supported by Hubei Health & Family Planning Commission Notice (No. [2017]20)Wuhan training project of the sixth batch of young and middle-aged medical talents, wuhan health & family planning commission (Wuhan Health & Family Planning Commission Notice No. [2018]116)Training project of the first batch of tanhualin famous doctors and students (Hubei TCM Hospital No. [2018]72)
文摘Objective:To explore core acupoints and acupoint selection principles in acupuncture and moxibustion for obesity,from syndrome differentiation prescriptions of the acupuncture-moxibustion therapy in 808 obesity prescriptions,by using node centrality and cluster analysis methods in complex network.Methods:Firstly,an acupoint network model is established,and acupoint nodes are assessed and calculated in multiple aspects by introducing the node centrality analysis idea of complex network,to excavate core acupoint nodes.Secondly,a cluster analysis is carried out on acupoint network by the cluster algorithm Q-PSO for complex network,to investigate the acupoint combination principles.Results:Zusanli(足三里ST36),Tianshu(天枢ST25),Fenglong(丰隆ST40),Zhongwan(中脘CV12)and Qihai(气海CV6),etc.,were included into the core acupoint Sanyinjiao(三阴交SP6)community.Zhigou(支沟TE6),Neiting(内庭ST44),Shangjuxu(上巨虚ST37),and Pishu(脾俞BL20)etc.,were included into the core acupoint Yinlingquan(阴陵泉SP9)community.Baihuanshu(白环俞BL30)and Zhiyang(至阳GV9)were included into the core acupoint Dachangshu(大肠俞BL25)community.Biguan(髀关ST31)was a single core community.Among all the acupoint nodes,SP6,ST25,SP9,ST36,CV6,Quchi(曲池L111),and Guanyuan(关元CV4)were of high degree centrality and eigenvector centrality,directly reflecting their importance in acupoint selection prescriptions.Conclusion:The Q-PSO algorithm is characterized with high precision and high efficiency,etc.The core acupoints and their combination principles explored by this algorithm are in accordance with clinical experiences.
