不同部位脂肪源性干细胞的生物学特性比较

背景: 大鼠不同部位来源的脂肪源性干细胞在体外培养时的特性是否存在差异目前尚无定论。目的: 比较同一只大鼠不同部位来源的脂肪源性干细胞在体外培养时的生长特性和成脂诱导分化能力的差异。方法: 无菌操作下取F344大鼠腹股沟及腹腔大网膜脂肪组织各5mL,Ⅰ型胶原酶酶解法分离出脂肪源性干细胞,细胞计数后进行体外培养,观察其形态特征和生长状态,MTT法测定不同部位细胞的倍增时间。取不同部位来源的第2代脂肪源性干细胞进行成脂诱导,诱导14d进行油红O染色,观察不同部位来源的脂肪源性干细胞的成脂分化能力。结果与结论: 在同一只大鼠内脏大网膜脂肪获得的脂肪源性干细胞数目为(281±10)×107L-1,明显多于腹股沟皮下脂肪的(85±5)×107L-1(P<0.01)。从内脏大网膜脂肪与腹股沟皮下脂肪获得的脂肪源性干细胞分别于第5,6天进入指数增长期;第9,10天到达平台期;倍增时间为50h和60h左右。传代后的细胞生长分化活跃,呈成纤维细胞样,成脂诱导后,大网膜组织来源的脂肪源性干细胞的成脂诱导分化率明显高于腹股沟组织来源的脂肪源性干细胞[(38.90±2.86)%,(35.30±3.29)%,P<0.01]。可见同一只大鼠不同部位脂肪组织分离得到的脂肪源性干细胞数目不同,体外成脂诱导分化能力亦存在差异。

Altered Nutrition State in the Severe Multiple Trauma Patients Undergoing Adjuvant Recombinant Human Growth Hormone Nutritional Support Therapy

In order to observe the nutrition state in the severe multiple trauma patients undergoing adjuvant recombinant human growth hormone (rhGH) nutritional support therapy, 45 patients with severe multiple traumas (ISS>25) were randomly divided into 3 groups. All the 3 groups had been supplied with nitrogen and caloricity according to the need of patients for 16 days. The rhGH therapy started 48 h after surgery and lasted for 14 days in two rhGH-treated groups in which rhGH was 0.2 and 0.4 U/(kg·d) respectively, and the resting group served as control one. The levels of nitrogen balance, prealbumin and safety variables (blood sugar, Na+, TT3 and TT4) were observed and com- pared among the three groups. The levels of nitrogen balance on the postoperative day (POD) 3 and 5 in the rhGH-treated groups were -1.28±3.19, 5.45±2.00 and -0.18±2.55, 6.11±1.60, respectively, which were significantly higher than those in the control group (-5.17±1.68 and -1.08±3.31, P<0.01). The values of prealbumin on the POD 3 and 5 in the rhGH-treated groups were 180.19±27.15, 194.44±50.82 and 194.94±29.65, 194.11±16.17, respectively, which were significantly higher than those in the control group (117.42±19.10 and 135.63±28.31, P<0.01). There was no sig- nificant difference between the rhGH 0.2 U/(kg·d) group and rhGH 0.4 U/(kg·d) group in both of the levels of nitrogen balance and prealbumin. It is concluded that the nutritional support therapy with adjuvant rhGH which starts 48 h after surgery improves the nutrition state of the patients with severe multiple trauma. It is safe for severe multiple trauma patients who accept rhGH at the dose of 0.2 and 0.4 U/(kg·d).

小容量高渗晶体等渗胶体溶液在创伤失血性休克早期容量复苏中应用的临床研究

目的观察小容量高渗晶体等渗胶体混合溶液对创伤失血性休克患者的早期容量复苏效果。方法将我科收治的48例早期轻、中度创伤失血性休克患者,随机分为两组,每组24例。通过外周血管在15～20 min 内快速输注250ml 研究药物(贺苏,7.2%氯化钠/6%羟乙基淀粉200/0.5注射液)或对照药物(贺斯,0.9%氯化钠/1%羟乙基淀粉200/0.5溶液)。在给药前、给药后0.5、10、15、20、25、30 min 测定患者的血压、脉搏和休克指数;在研究开始前和给药后30 min 各采集1次外周血样本,并检测血常规、血 Na^+、Cl^-和凝血常规。结果研究组收缩压在给药后0、5、10、15、20、25、30 min 均显著高于对照组(P<0.01),舒张压在给药后0、5、10、15 min 内均显著高于对照组(P<0.05),休克指数在给药后均显著低于对照组(P<0.05)。研究组在给药后血 Na^+、Cl^-高于对照组(P<0.01),但其平均浓度在正常参考值范围内。研究组与对照组凝血功能、血常规指标差异均无统计学差异。结论小容量高渗晶体等渗胶溶液在创伤失血性休克患者早期血流动力学快速恢复作用上有良好的效果且安全性良好。

BALB/c小鼠胚胎皮肤无瘢痕愈合相关基因的筛选与克隆

目的在已构建成年小鼠和小鼠胚胎皮肤差异表达的c DNA文库基础上,通过双向抑制性消减杂交筛选BALB/c小鼠胚胎皮肤无瘢痕愈合相关基因。方法应用双向抑制性消减杂交(suppressionsubtractivehybridization,SSH)技术,筛选BALB/c小鼠胚胎皮肤与BALB/c成年小鼠皮肤c DNA片段。结果获得16个在鼠胚皮肤中特异表达的无瘢痕愈合相关基因的c DNA片段,7个在成鼠皮肤中特异表达的无瘢痕愈合相关基因的c DNA片段。其中有2个新发现与无瘢痕愈合相关基因未见文献报道。结论研究证实确有部分基因在鼠胚皮肤和成鼠皮肤中分别特异表达,这种差异表达极有可能是哺乳动物胚胎皮肤无瘢痕愈合的分子生物学基础。