Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,inclu...Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season.展开更多
Ulvacean green seaweeds are common worldwide;they formed massive green tides in the Yellow Sea in recent years,which caused marine ecological problems as well as a social issue.We investigated two major genera of the ...Ulvacean green seaweeds are common worldwide;they formed massive green tides in the Yellow Sea in recent years,which caused marine ecological problems as well as a social issue.We investigated two major genera of the Ulvaceae,Ulva and Enteromorpha,and collected the plastid rbcL and nuclear ITS sequences of specimens of the genera in two sides of the Yellow Sea and analyzed them.Phylogenetic trees of rbcL data show the occurrence of five species of Enteromorpha(E.compressa,E.flexuosa,E.intestinalis,E.linza and E.prolifera) and three species of Ulva(U.pertusa,U.rigida and U.ohnoi).However,we found U.ohnoi,which is known as a subtropical to tropical species,at two sites on Jeju Island,Korea.Four ribotypes in partial sequences of 5.8S rDNA and ITS2 from E.compressa were also found.Ribotype network analysis revealed that the common ribotype,occurring in China,Korea and Europe,is connected with ribotypes from Europe and China/Japan.Although samples of the same species were collected from both sides of the Yellow Sea,intraspecific genetic polymorphism of each species was low among samples collected worldwide.展开更多
Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The result...Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.展开更多
基金Supported by NSFC (40506030)the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209)Science & Technology Project of Qingdao City (06-2-2-12-JCH)
文摘Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season.
基金Supported by the National Key Technology Research and Development Program(No.2008BAC49B01)National High Technology Research and Development Program of China(863 Program)(No.2009AA10Z106)the Knowledge Innovation Project of the Chinese Academy of Sciences(No.KZCX2-YW-209)
文摘Ulvacean green seaweeds are common worldwide;they formed massive green tides in the Yellow Sea in recent years,which caused marine ecological problems as well as a social issue.We investigated two major genera of the Ulvaceae,Ulva and Enteromorpha,and collected the plastid rbcL and nuclear ITS sequences of specimens of the genera in two sides of the Yellow Sea and analyzed them.Phylogenetic trees of rbcL data show the occurrence of five species of Enteromorpha(E.compressa,E.flexuosa,E.intestinalis,E.linza and E.prolifera) and three species of Ulva(U.pertusa,U.rigida and U.ohnoi).However,we found U.ohnoi,which is known as a subtropical to tropical species,at two sites on Jeju Island,Korea.Four ribotypes in partial sequences of 5.8S rDNA and ITS2 from E.compressa were also found.Ribotype network analysis revealed that the common ribotype,occurring in China,Korea and Europe,is connected with ribotypes from Europe and China/Japan.Although samples of the same species were collected from both sides of the Yellow Sea,intraspecific genetic polymorphism of each species was low among samples collected worldwide.
基金Supported by the Key Innovation Project of Institute of Oceanology,Chinese Academy of Sciences(No.2009-2)the Natural Science Foundation of Shandong Province(No.2009ZRB02542)+2 种基金the Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques,SOA(No.MBSMAT-2010-03)the National Natural Science Foundation of China(No.41276164)the Natural Science Foundation of Jiangsu Province(No.BK2012650)
文摘Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
