In this work,the effect of alcohols (including alcohols containing single, two, three hydroxyl groups, respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique...In this work,the effect of alcohols (including alcohols containing single, two, three hydroxyl groups, respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique of enzymatic catalysis in a heterogeneous pseudo-eutectic solid-to-solid mixture with thermolysin.The experimental result shows that the alcohols containing two hydroxyl groups are the most suitable adjuncts for the studied enzymatic synthesis system, and the yield of ZAPM for 1,4-butanediol as an adjunct reaches 95% at reaction time 2?h, which is better than the results in two-phase systems and other pseudo-eutectic solid-to-solid mixture reported in literature.展开更多
基金国家自然科学基金 (No .2 9976 0 19)江苏省自然科学基金 (No .BK9912 3)资助项目~~
文摘In this work,the effect of alcohols (including alcohols containing single, two, three hydroxyl groups, respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique of enzymatic catalysis in a heterogeneous pseudo-eutectic solid-to-solid mixture with thermolysin.The experimental result shows that the alcohols containing two hydroxyl groups are the most suitable adjuncts for the studied enzymatic synthesis system, and the yield of ZAPM for 1,4-butanediol as an adjunct reaches 95% at reaction time 2?h, which is better than the results in two-phase systems and other pseudo-eutectic solid-to-solid mixture reported in literature.
