目的探究Zn_(x)Ni_(1-x)S@DOX纳米结构在肿瘤光热-化学疗法联合治疗中的应用,为肿瘤治疗方法的研究提供新思路。方法通过透射电子显微镜等方法对Zn_(x)Ni_(1-x)S纳米结构进行表征测试;通过红外热成像仪探究光热性能;通过荧光光谱仪研究...目的探究Zn_(x)Ni_(1-x)S@DOX纳米结构在肿瘤光热-化学疗法联合治疗中的应用,为肿瘤治疗方法的研究提供新思路。方法通过透射电子显微镜等方法对Zn_(x)Ni_(1-x)S纳米结构进行表征测试;通过红外热成像仪探究光热性能;通过荧光光谱仪研究了不同温度和不同pH条件下Zn_(x)Ni_(1-x)S@DOX纳米结构的药物释放情况;通过MTT实验探究Zn_(x)Ni_(1-x)S纳米结构的生物安全性及Zn_(x)Ni_(1-x)S@DOX纳米结构的体外抗肿瘤性能。结果所制备的Zn_(x)Ni_(1-x)S纳米结构分散性良好且尺寸均一;光热性能测试结果显示Zn x Ni 1-x S纳米结构具有良好的光热升温效果;Zn_(x)Ni_(1-x)S@DOX纳米结构的药物释放行为具有温度和pH响应性;细胞实验表明,Zn_(x)Ni_(1-x)S纳米结构对正常细胞无明显毒性,在近红外光照射下,Zn_(x)Ni_(1-x)S@DOX纳米结构能够显著地抑制肿瘤细胞的生长。结论Zn_(x)Ni_(1-x)S纳米结构是一种生物相容性良好的纳米材料,在此基础上构建的Zn_(x)Ni_(1-x)S@DOX纳米结构具有良好的抗肿瘤性能,能够用于肿瘤的光热-化学疗法联合治疗研究。展开更多
We report that atomically thin two-dimensional silicon quantum sheets(2D Si QSs),prepared by a scalable approach coupling chemical delithiation and cryo-assisted exfoliation,can serve as a highperformance brain photon...We report that atomically thin two-dimensional silicon quantum sheets(2D Si QSs),prepared by a scalable approach coupling chemical delithiation and cryo-assisted exfoliation,can serve as a highperformance brain photonic nanoagent for orthotopic glioma theranostics.With the lateral size of approximately 14.0 nm and thickness of about 1.6 nm,tiny Si QSs possess high mass extinction coefficient of 27.5 Lg^(-1)cm^(-1)and photothermal conversion efficiency of 47.2%at 808 nm,respectively,concurrently contributing to the best photothermal performance among the reported 2 D mono-elemental materials(Xenes).More importantly,Si QSs with low toxicity maintain the trade-off between stability and degradability,paving the way for practical clinical translation in consideration of both storage and action of nanoagents.In vitro Transwell filter experiment reveals that Si QSs could effectively go across the b End.3 cells monolayer.Upon the intravenous injection of Si QSs,orthotopic brain tumors are effectively inhibited under the precise guidance of photoacoustic imaging,and the survival lifetime of brain tumor-bearing mice is increased by two fold.Atomically thin Si QSs with strong light-harvesting capability are expected to provide an effective and robust 2D photonic nanoplatform for the management of brain diseases.展开更多
文摘目的探究Zn_(x)Ni_(1-x)S@DOX纳米结构在肿瘤光热-化学疗法联合治疗中的应用,为肿瘤治疗方法的研究提供新思路。方法通过透射电子显微镜等方法对Zn_(x)Ni_(1-x)S纳米结构进行表征测试;通过红外热成像仪探究光热性能;通过荧光光谱仪研究了不同温度和不同pH条件下Zn_(x)Ni_(1-x)S@DOX纳米结构的药物释放情况;通过MTT实验探究Zn_(x)Ni_(1-x)S纳米结构的生物安全性及Zn_(x)Ni_(1-x)S@DOX纳米结构的体外抗肿瘤性能。结果所制备的Zn_(x)Ni_(1-x)S纳米结构分散性良好且尺寸均一;光热性能测试结果显示Zn x Ni 1-x S纳米结构具有良好的光热升温效果;Zn_(x)Ni_(1-x)S@DOX纳米结构的药物释放行为具有温度和pH响应性;细胞实验表明,Zn_(x)Ni_(1-x)S纳米结构对正常细胞无明显毒性,在近红外光照射下,Zn_(x)Ni_(1-x)S@DOX纳米结构能够显著地抑制肿瘤细胞的生长。结论Zn_(x)Ni_(1-x)S纳米结构是一种生物相容性良好的纳米材料,在此基础上构建的Zn_(x)Ni_(1-x)S@DOX纳米结构具有良好的抗肿瘤性能,能够用于肿瘤的光热-化学疗法联合治疗研究。
基金supported by the National Key Research and Development Program of China:Scientific and Technological Innovation Cooperation of Mainland and Macao(2017YFE0120000)the National Natural Science Foundation of China(31800834,81527901,81571745,91859117,81771906,81827807,81901812,and 52071120)+6 种基金the Open Foundation of Shenzhen Bay Laboratory(SZBL2019062801005)the Fundamental Research Funds for the Central Universities(JZ2020HGTB0031 and JZ2018HGPA0273)the Science and Technology Key Project of Shenzhen(JCYJ20160229200902680)the Shenzhen Key Laboratory of Ultrasound Imaging and Therapy(ZDSYS201802061806314)the Shenzhen Double Chain Grant([2018]256)the Natural Science Foundation of Guangdong Province(2014A030312006)the China Postdoctoral Science Foundation(2019 M653129)。
文摘We report that atomically thin two-dimensional silicon quantum sheets(2D Si QSs),prepared by a scalable approach coupling chemical delithiation and cryo-assisted exfoliation,can serve as a highperformance brain photonic nanoagent for orthotopic glioma theranostics.With the lateral size of approximately 14.0 nm and thickness of about 1.6 nm,tiny Si QSs possess high mass extinction coefficient of 27.5 Lg^(-1)cm^(-1)and photothermal conversion efficiency of 47.2%at 808 nm,respectively,concurrently contributing to the best photothermal performance among the reported 2 D mono-elemental materials(Xenes).More importantly,Si QSs with low toxicity maintain the trade-off between stability and degradability,paving the way for practical clinical translation in consideration of both storage and action of nanoagents.In vitro Transwell filter experiment reveals that Si QSs could effectively go across the b End.3 cells monolayer.Upon the intravenous injection of Si QSs,orthotopic brain tumors are effectively inhibited under the precise guidance of photoacoustic imaging,and the survival lifetime of brain tumor-bearing mice is increased by two fold.Atomically thin Si QSs with strong light-harvesting capability are expected to provide an effective and robust 2D photonic nanoplatform for the management of brain diseases.
