Objective： To study the serum protein fingerprint of patients with pancreatic cancer and to screen for protein molecules closely related to pancreatic cancer during the onset and progression of the disease using surf...Objective： To study the serum protein fingerprint of patients with pancreatic cancer and to screen for protein molecules closely related to pancreatic cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time of fight mass spectrometry （SELDI-TOF-MS）. Methods： Serum samples from 20 pancreatic cancers, 20 healthy volunteers and 18 patients with other pancreatic diseases. WCX magnetic beans and PBSII-C protein chips reader （Ciphergen Biosystems Ins.） were used. The protein fingerprint expression of all the Serum samples and the resulting profiles between cancer and normal were analyzed with BiomarkerWizard system. Results： Agroup ofproteomic peaks were detected. Four differently expressed potential biomarkers were identified with the relative molecular weights of 5705 Da, 4935 Da, 5318 Da and 3243 Da. Among them, two proteins with m/z5705, 5318Da down-regulated, and two proteins with m/z 4935, 3243 Da were up-regulated in pancreatic cancers. Conclusion： SELDI technology can be used to screen significant proteins of differential expression in the serum of pancreatic cancer patients. These different proteins could be specific biomarkers of the patients with pancreatic cancer in the serum and have the potential value of further investigation.展开更多
Objective： To search the specific serum proteins in tumor-like polypoid lesions of the gallbladder（PLG） patients. Methods： Surface enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF...Objective： To search the specific serum proteins in tumor-like polypoid lesions of the gallbladder（PLG） patients. Methods： Surface enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF-MS） technique and WCX Magnetic Beads were used to detect the serum proteomic fingerprint of 23 patients with tumor-like PLG, 21 patients with non tumor-like PLG and 26 normal persons. Biomarker Wizard and Biomarker Patterns Software were used in combination to analyze the data. Results： In the preliminary screening, 22 representative specific proteins for tumor-like PLG were found. Seven specific proteins showed increased expression and 15 specific proteins showed decreased expression in the tumor-like PLG patients. Three specific proteins are selected for the diagnosis of the tumor-like PLG.. Conclusion： SELDI-TOF-MS technique can be used to select specific proteins for tumor-like PLG patients, which may be useful for the diagnosis of the tumor-like PLG and the differential diagnosis with the non tumor-like PLG.展开更多
胆囊息肉样病变（polypoid lesions of gallbladder，PLG）是一类表现为胆囊黏膜向腔内突起的局限性息肉样突起病变的总称。此类疾病包含良、恶性病变20余种。随着B超检查的普及，胆囊息肉样病变在人群中的检出率已达1％，在临床中已成...胆囊息肉样病变（polypoid lesions of gallbladder，PLG）是一类表现为胆囊黏膜向腔内突起的局限性息肉样突起病变的总称。此类疾病包含良、恶性病变20余种。随着B超检查的普及，胆囊息肉样病变在人群中的检出率已达1％，在临床中已成为一种外科常见病。由于胆囊息肉样病变包含的疾病种类多，手术前明确病变的性质困难，如何对待和处理胆囊息肉样病变，对手术治疗的时机选择，展开更多
基金a grant from the Sci & Tech Foundation of Liaoning Province (No.2005225003-3).
文摘Objective： To study the serum protein fingerprint of patients with pancreatic cancer and to screen for protein molecules closely related to pancreatic cancer during the onset and progression of the disease using surface-enhanced laser desorption and ionization time of fight mass spectrometry （SELDI-TOF-MS）. Methods： Serum samples from 20 pancreatic cancers, 20 healthy volunteers and 18 patients with other pancreatic diseases. WCX magnetic beans and PBSII-C protein chips reader （Ciphergen Biosystems Ins.） were used. The protein fingerprint expression of all the Serum samples and the resulting profiles between cancer and normal were analyzed with BiomarkerWizard system. Results： Agroup ofproteomic peaks were detected. Four differently expressed potential biomarkers were identified with the relative molecular weights of 5705 Da, 4935 Da, 5318 Da and 3243 Da. Among them, two proteins with m/z5705, 5318Da down-regulated, and two proteins with m/z 4935, 3243 Da were up-regulated in pancreatic cancers. Conclusion： SELDI technology can be used to screen significant proteins of differential expression in the serum of pancreatic cancer patients. These different proteins could be specific biomarkers of the patients with pancreatic cancer in the serum and have the potential value of further investigation.
基金supported by the Scientific Research Foundation of the High School from Education Department of Liaoning Province(No.20060923).
文摘Objective： To search the specific serum proteins in tumor-like polypoid lesions of the gallbladder（PLG） patients. Methods： Surface enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF-MS） technique and WCX Magnetic Beads were used to detect the serum proteomic fingerprint of 23 patients with tumor-like PLG, 21 patients with non tumor-like PLG and 26 normal persons. Biomarker Wizard and Biomarker Patterns Software were used in combination to analyze the data. Results： In the preliminary screening, 22 representative specific proteins for tumor-like PLG were found. Seven specific proteins showed increased expression and 15 specific proteins showed decreased expression in the tumor-like PLG patients. Three specific proteins are selected for the diagnosis of the tumor-like PLG.. Conclusion： SELDI-TOF-MS technique can be used to select specific proteins for tumor-like PLG patients, which may be useful for the diagnosis of the tumor-like PLG and the differential diagnosis with the non tumor-like PLG.
文摘胆囊息肉样病变（polypoid lesions of gallbladder，PLG）是一类表现为胆囊黏膜向腔内突起的局限性息肉样突起病变的总称。此类疾病包含良、恶性病变20余种。随着B超检查的普及，胆囊息肉样病变在人群中的检出率已达1％，在临床中已成为一种外科常见病。由于胆囊息肉样病变包含的疾病种类多，手术前明确病变的性质困难，如何对待和处理胆囊息肉样病变，对手术治疗的时机选择，
