This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labelling agents. The OI-RD intensities not only dep...This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.展开更多
Oblique-incidence reflectivity difference (OI-RD) analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not...Oblique-incidence reflectivity difference (OI-RD) analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential applications in detection of biochemical processes.展开更多
基金Project supported by the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant No L3FX051W)
文摘This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.
文摘Oblique-incidence reflectivity difference (OI-RD) analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential applications in detection of biochemical processes.